Staphylococcus aureus nasal carriage among healthcare workers in Kisangani, the Democratic Republic of the Congo

H De Boeck, S Vandendriessche, M Hallin, B Batoko, J-P Alworonga, B Mapendo, C Van Geet, N Dauly, O Denis, J Jacobs

Research output: Contribution to journalA1: Web of Science-article

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a global health concern, but there are few data from Central Africa. The objective of our study was to characterise S. aureus colonisation isolates from healthcare-exposed professionals in the Democratic Republic of the Congo (DRC). Healthcare workers and medical students (n = 380) in Kisangani, DRC were screened for S. aureus nasal carriage in a single-centre cross-sectional study in the University Hospital of Kisangani. The isolates were identified and characterised using phenotypic and genotypic methods. The nasal carriage rate of S. aureus was 16.6 % and 10 out of 63 isolates (15.9 %) were MRSA. We found 28 different spa types. Most MRSA isolates belonged to ST8-spa t1476-SCCmec V. The majority of MRSA were multidrug-resistant to non-beta-lactam antibiotics. Overall, 28.5 % of S. aureus carried Panton-Valentine leucocidin (PVL)-encoding genes (all methicillin-sensitive) and 17.5 % carried toxic shock syndrome toxin-1 (TSST-1)-encoding genes. The finding of MRSA carriage among healthcare workers in a setting with limited access to diagnostic microbiology and appropriate therapy calls for improved education on infection control practices and supports the introduction of surveillance programmes.

Original languageEnglish
JournalEuropean Journal of Clinical Microbiology and Infectious Diseases
Volume34
Issue number8
Pages (from-to)1567-1572
Number of pages6
ISSN0934-9723
DOIs
Publication statusPublished - 2015

Keywords

  • Adult
  • Bacterial Toxins
  • Carrier State
  • Cross-Sectional Studies
  • Democratic Republic of the Congo
  • Drug Resistance, Bacterial
  • Enterotoxins
  • Exotoxins
  • Female
  • Genotype
  • Health Personnel
  • Hospitals, University
  • Humans
  • Leukocidins
  • Male
  • Middle Aged
  • Molecular Typing
  • Nasal Mucosa
  • Staphylococcal Infections
  • Staphylococcus aureus
  • Students, Medical
  • Superantigens
  • Young Adult
  • Journal Article
  • Research Support, Non-U.S. Gov't

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