Epidemiological investigations on Trypanosoma evansi infection in dromedary camels in the South of Algeria

Djamila Boushaki; Amel Adel; Mamadou Lamine Dia; Philippe Büscher; Hafsa Madani; Brahim Aymard Brihoum; Hassiba Sadaoui; Nadera Bouayed; Nadia Kechemir Issad

doi:10.1016/j.heliyon.2019.e02086

Epidemiological investigations on Trypanosoma evansi infection in dromedary camels in the South of Algeria

Djamila Boushaki, Amel Adel, Mamadou Lamine Dia, Philippe Büscher, Hafsa Madani, Brahim Aymard Brihoum, Hassiba Sadaoui, Nadera Bouayed, Nadia Kechemir Issad

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Abstract

An epidemiological study of Trypanosoma evansi ( T. evansi) infection in dromedaries was conducted in four wilayate (localities) of Southern Algeria: Béchar, El Bayadh, Ouargla, Tamanrasset. Between February 2014 and April 2016, 1056 camels of different ages and both sexes from 84 herds were sampled. The prevalence was determined through parasitological examination (Giemsa stained thin smear, GST), serological tests (CATT/ T. evansi, ELISA/VSG RoTat 1.2, immune trypanolysis), and molecular tests ( T. evansi type A specific RoTat 1.2 PCR and T. evansi type B specific EVAB PCR). The overall prevalence was 2.4 % with GST, 32.4% with CATT/ T. evansi, 23.1% with ELISA/VSG RoTat 1.2, 21.0% with immune trypanolysis (TL), 11.2 % with RoTat 1.2 PCR and 0% with EVAB PCR. El Bayadh was the most affected wilaya with 11.8% positives in GST, 74.9% in CATT/ T. evansi, 70.1% in ELISA/VSG RoTat 1.2 and 62.2% in immune trypanolysis. Only in Béchar, a non-significantly higher prevalence (13.6%) was observed with RoTat1.2 PCR than in El Bayadh (13.0%). We didn't find any evidence of the presence of T. evansi type B in the study area.

Original language	English
Article number	e02086
Journal	Heliyon
Volume	5
Issue number	7
Number of pages	8
ISSN	2405-8440
DOIs	https://doi.org/10.1016/j.heliyon.2019.e02086
Publication status	Published - 2019

Keywords

Zoology
Algeria
Trypanosoma evansi
Surra
Dromedary camel
GST
RoTat 1.2 PCR
CATT/T. evansi
ELISA/VSG RoTat 1.2
Immune trypanolysis
SEROLOGICAL TESTS
HIGH AGREEMENT
LOW KAPPA
DIAGNOSIS
EXPRESSION
PREVALENCE
RESISTANCE
MOROCCO
ANTIGEN
DRUG

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@article{9543aedff3094b0abab683193fd841d9,

title = "Epidemiological investigations on Trypanosoma evansi infection in dromedary camels in the South of Algeria",

abstract = "An epidemiological study of Trypanosoma evansi ( T. evansi) infection in dromedaries was conducted in four wilayate (localities) of Southern Algeria: B{\'e}char, El Bayadh, Ouargla, Tamanrasset. Between February 2014 and April 2016, 1056 camels of different ages and both sexes from 84 herds were sampled. The prevalence was determined through parasitological examination (Giemsa stained thin smear, GST), serological tests (CATT/ T. evansi, ELISA/VSG RoTat 1.2, immune trypanolysis), and molecular tests ( T. evansi type A specific RoTat 1.2 PCR and T. evansi type B specific EVAB PCR). The overall prevalence was 2.4 % with GST, 32.4% with CATT/ T. evansi, 23.1% with ELISA/VSG RoTat 1.2, 21.0% with immune trypanolysis (TL), 11.2 % with RoTat 1.2 PCR and 0% with EVAB PCR. El Bayadh was the most affected wilaya with 11.8% positives in GST, 74.9% in CATT/ T. evansi, 70.1% in ELISA/VSG RoTat 1.2 and 62.2% in immune trypanolysis. Only in B{\'e}char, a non-significantly higher prevalence (13.6%) was observed with RoTat1.2 PCR than in El Bayadh (13.0%). We didn't find any evidence of the presence of T. evansi type B in the study area. ",

keywords = "Zoology, Algeria, Trypanosoma evansi, Surra, Dromedary camel, GST, RoTat 1.2 PCR, CATT/T. evansi, ELISA/VSG RoTat 1.2, Immune trypanolysis, SEROLOGICAL TESTS, HIGH AGREEMENT, LOW KAPPA, DIAGNOSIS, EXPRESSION, PREVALENCE, RESISTANCE, MOROCCO, ANTIGEN, DRUG",

author = "Djamila Boushaki and Amel Adel and Dia, {Mamadou Lamine} and Philippe B{\"u}scher and Hafsa Madani and Brihoum, {Brahim Aymard} and Hassiba Sadaoui and Nadera Bouayed and Issad, {Nadia Kechemir}",

note = "FTX; DOAJ; (CC BY-NC-ND 4.0)",

year = "2019",

doi = "10.1016/j.heliyon.2019.e02086",

language = "English",

volume = "5",

journal = "Heliyon",

issn = "2405-8440",

publisher = "Elsevier",

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TY - JOUR

T1 - Epidemiological investigations on Trypanosoma evansi infection in dromedary camels in the South of Algeria

AU - Boushaki, Djamila

AU - Adel, Amel

AU - Dia, Mamadou Lamine

AU - Büscher, Philippe

AU - Madani, Hafsa

AU - Brihoum, Brahim Aymard

AU - Sadaoui, Hassiba

AU - Bouayed, Nadera

AU - Issad, Nadia Kechemir

N1 - FTX; DOAJ; (CC BY-NC-ND 4.0)

PY - 2019

Y1 - 2019

N2 - An epidemiological study of Trypanosoma evansi ( T. evansi) infection in dromedaries was conducted in four wilayate (localities) of Southern Algeria: Béchar, El Bayadh, Ouargla, Tamanrasset. Between February 2014 and April 2016, 1056 camels of different ages and both sexes from 84 herds were sampled. The prevalence was determined through parasitological examination (Giemsa stained thin smear, GST), serological tests (CATT/ T. evansi, ELISA/VSG RoTat 1.2, immune trypanolysis), and molecular tests ( T. evansi type A specific RoTat 1.2 PCR and T. evansi type B specific EVAB PCR). The overall prevalence was 2.4 % with GST, 32.4% with CATT/ T. evansi, 23.1% with ELISA/VSG RoTat 1.2, 21.0% with immune trypanolysis (TL), 11.2 % with RoTat 1.2 PCR and 0% with EVAB PCR. El Bayadh was the most affected wilaya with 11.8% positives in GST, 74.9% in CATT/ T. evansi, 70.1% in ELISA/VSG RoTat 1.2 and 62.2% in immune trypanolysis. Only in Béchar, a non-significantly higher prevalence (13.6%) was observed with RoTat1.2 PCR than in El Bayadh (13.0%). We didn't find any evidence of the presence of T. evansi type B in the study area.

AB - An epidemiological study of Trypanosoma evansi ( T. evansi) infection in dromedaries was conducted in four wilayate (localities) of Southern Algeria: Béchar, El Bayadh, Ouargla, Tamanrasset. Between February 2014 and April 2016, 1056 camels of different ages and both sexes from 84 herds were sampled. The prevalence was determined through parasitological examination (Giemsa stained thin smear, GST), serological tests (CATT/ T. evansi, ELISA/VSG RoTat 1.2, immune trypanolysis), and molecular tests ( T. evansi type A specific RoTat 1.2 PCR and T. evansi type B specific EVAB PCR). The overall prevalence was 2.4 % with GST, 32.4% with CATT/ T. evansi, 23.1% with ELISA/VSG RoTat 1.2, 21.0% with immune trypanolysis (TL), 11.2 % with RoTat 1.2 PCR and 0% with EVAB PCR. El Bayadh was the most affected wilaya with 11.8% positives in GST, 74.9% in CATT/ T. evansi, 70.1% in ELISA/VSG RoTat 1.2 and 62.2% in immune trypanolysis. Only in Béchar, a non-significantly higher prevalence (13.6%) was observed with RoTat1.2 PCR than in El Bayadh (13.0%). We didn't find any evidence of the presence of T. evansi type B in the study area.

KW - Zoology

KW - Algeria

KW - Trypanosoma evansi

KW - Surra

KW - Dromedary camel

KW - GST

KW - RoTat 1.2 PCR

KW - CATT/T. evansi

KW - ELISA/VSG RoTat 1.2

KW - Immune trypanolysis

KW - SEROLOGICAL TESTS

KW - HIGH AGREEMENT

KW - LOW KAPPA

KW - DIAGNOSIS

KW - EXPRESSION

KW - PREVALENCE

KW - RESISTANCE

KW - MOROCCO

KW - ANTIGEN

KW - DRUG

U2 - 10.1016/j.heliyon.2019.e02086

DO - 10.1016/j.heliyon.2019.e02086

M3 - A1: Web of Science-article

C2 - 31372547

SN - 2405-8440

VL - 5

JO - Heliyon

JF - Heliyon

IS - 7

M1 - e02086

ER -

Epidemiological investigations on Trypanosoma evansi infection in dromedary camels in the South of Algeria

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Keywords

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