Eradication of hantavirus infection among laboratory rats by application of caesarian section and a foster mother technique

Hantavirus antibodies were demonstrated by the indirect immunofluorescent antibody assay, in the serum of inbred strains of laboratory rats, during the period 1973–1982, at the Unit of Experimental Immunology in the Catholic University of Louvain, Brussels, Belgium.
LOU rats, as well as immunocytomas, which were requested by laboratories in the U.K. and The Netherlands, were supplied at a time when the infection was unknown and unsuspected in Europe.
Hantavirus-infected laboratory rats were rendered free of virus through rederivation by caesarian section and suckling by virus-free foster mothers.
Immunocytomas were tested for the presence of hantaviruses by implantation into seronegative laboratory rats. The strain of hantavirus causing the laboratory infection was clearly different from the one circulating in free-living bankvoles in Belgium.
The exchange of laboratory rats and rat tumours in relation to the potential risk of laboratory-acquired hantavirus infection, is discussed.