Evaluation of an antibody detecting point of care test for diagnosis of Taenia solium cysticercosis in a Zambian rural community: a prospective diagnostic accuracy study

Chishimba Mubanga, Inge Van Damme, Chiara Trevisan, Veronika Schmidt, Isaac K. Phiri, Gideon Zulu, John Noh, Sukwan Handali, Richard Mambo, Mwelwa Chembensofu, Maxwell Masuku, Dries Reynders, Famke Jansen, Emmanuel Bottieau, Pascal Magnussen, Andrea S. Winkler, Pierre Dorny, Kabemba E. Mwape, Sarah Gabriël

Research output: Contribution to journalA1: Web of Science-articlepeer-review

Abstract

The lack of cheap, easy-to-use, rapid diagnostic tests has led to the development of several rapid diagnostic tests for cysticercosis. The new prototype two-strip, Taenia solium point of care test (TS POC) detects antibodies against taeniosis (TS POC T) and cysticercosis (TS POC CC). This study evaluated the diagnostic performance of the TS POC CC in the Sinda district in eastern Zambia. A sample of 1254 participants was recruited and tested with the TS POC. Out of the 1249 participants with a valid TS POC result, 177 (14%) tested positive while 1072 (86%) tested negative. All individuals with a positive TS POC and a subset of negative TS POC participants were selected for serum sampling, and were subjected to the recombinant glycoprotein T24H enzyme-linked immunoelectrotransfer blot (rT24H EITB) and the serum B60/158 (serum Ag) enzyme-linked immunosorbent assay (Ag ELISA). Performance characteristics were estimated using a Bayesian approach with probabilistic constraints. Based on 255 complete cases, the estimated sensitivity and specificity of the TS POC CC test were 35% (95% CI: 14-63%) and 87% (95% CI: 83-90%), respectively. The diagnostic performance needs to be improved, possibly by titrating antigen and other reagents' concentration in the strip to produce a performance similar to existing cysticercosis tests such as the rT24H EITB.

Original languageEnglish
Article number2121
JournalDiagnostics
Volume11
Issue number11
Number of pages12
DOIs
Publication statusPublished - 2021

Keywords

  • Taenia solium
  • human cysticercosis
  • point of care test
  • diagnosis
  • screening
  • antigen ELISA
  • rT24H EITB
  • sensitivity
  • specificity
  • IMMUNOELECTROTRANSFER BLOT ASSAY
  • PREVALENCE
  • ANTIGEN
  • FRAMEWORK
  • CLONING

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