Evaluation of the AnTat A/B and LiTat A/B primers for the detection of Trypanosoma brucei gambiense

Elimination of gambiense human African trypanosomiasis (gHAT) as a public health problem has been reached or is in sight in a number of endemic foci and the next step is now to reach the elimination of transmission. The ability to detect Trypanosoma brucei gambiense (T. b. gambiense) in both the last human cases and in a suspected animal reservoir becomes increasingly important to reach this goal. We have evaluated here the diagnostic performance of the AnTat A/B and LiTat A/B primers in comparison with the TBR, TgsGP and nested TgsGP PCRs that are currently used for the molecular diagnosis of gHAT. The evaluation was based on serial DNA dilutions from two T. b. gambiense strains for sensitivity, purified reference strains for specificity and field strains isolated from pigs in Côte d’Ivoire for field application. Results showed that the two PCRs (AnTat A/B and LiTat A/B) are not specific for T. b. gambiense, limiting their relevance for studies on suspected animal reservoirs. However, they could represent complementary tools to improve the molecular diagnosis of gHAT in the elimination process even if the detection limit was lowest than for the TgsGP PCR. The results also once more suggest that nested TgsGP PCR should be interpreted with caution as they may lead to an over-estimation of the T. b. gambiense prevalence particularly in animal studies.