TY - JOUR
T1 - Evaluation of the CellaVision DM96 advanced RBC application for screening and follow-up of malaria infection
AU - Florin, Lisa
AU - Maelegheer, Karel
AU - Muyldermans, Astrid
AU - Van Esbroeck, Marjan
AU - Nulens, Eric
AU - Emmerechts, Jan
N1 - NPP
PY - 2018
Y1 - 2018
N2 - CellaVision DM96 is a digital cell morphology system for automated classification of white and red blood cells. CellaVision Advanced RBC application (ARBCA) pre-classifies RBC in 21 categories, including parasitized RBC, and allows re-classification by the operator. In this study, the performance of the software for detection of malaria and calculation of parasitemia was evaluated and compared to microscopy (n = 40). For CellaVision, both pre- and post-reclassification results were evaluated. Sensitivity was moderate, even post-reclassification (72%), due to low numbers of analyzed RBC and limited resolution of photographs. CellaVision results correlated with microscopy according to Passing-Bablok analysis, with slightly lower values for CellaVision. Within-run, between-run and inter-observer variability were acceptable. The low sensitivity of CellaVision ARBCA precludes its use as a screening technique for malaria. However, due to its good correlation with microscopy and short turnaround-times, it may be useful in follow-up of parasitemia. Larger studies are required to confirm these findings. (C) 2017 Elsevier Inc. All rights reserved.
AB - CellaVision DM96 is a digital cell morphology system for automated classification of white and red blood cells. CellaVision Advanced RBC application (ARBCA) pre-classifies RBC in 21 categories, including parasitized RBC, and allows re-classification by the operator. In this study, the performance of the software for detection of malaria and calculation of parasitemia was evaluated and compared to microscopy (n = 40). For CellaVision, both pre- and post-reclassification results were evaluated. Sensitivity was moderate, even post-reclassification (72%), due to low numbers of analyzed RBC and limited resolution of photographs. CellaVision results correlated with microscopy according to Passing-Bablok analysis, with slightly lower values for CellaVision. Within-run, between-run and inter-observer variability were acceptable. The low sensitivity of CellaVision ARBCA precludes its use as a screening technique for malaria. However, due to its good correlation with microscopy and short turnaround-times, it may be useful in follow-up of parasitemia. Larger studies are required to confirm these findings. (C) 2017 Elsevier Inc. All rights reserved.
KW - CellaVision
KW - Malaria
KW - Diagnosis
KW - Parasitemia
U2 - 10.1016/j.diagmicrobio.2017.12.002
DO - 10.1016/j.diagmicrobio.2017.12.002
M3 - A1: Web of Science-article
SN - 0732-8893
VL - 90
SP - 253
EP - 256
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
IS - 4
ER -