Evaluation of the intercept oral specimen collection device with HIV assays versus paired serum/plasma specimens

Greta Beelaert; L Van Heddegem; M Van Frankenhuijsen; G Vandewalle; V Compernolle; E Florence; K Fransen

doi:10.1016/j.jviromet.2016.04.014

Evaluation of the intercept oral specimen collection device with HIV assays versus paired serum/plasma specimens

Greta Beelaert, L Van Heddegem, M Van Frankenhuijsen, G Vandewalle, V Compernolle, E Florence, K Fransen

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

Oral fluid has many advantages over blood-based techniques: it is less invasive, eliminates the occupational risk associated with needle stick accidents and collection can be self-administrated. Each individual test is packaged with a corresponding collection device. This study tested the suitability of the Intercept Oral Specimen Collection Device for different HIV diagnostic tests: three different rapid HIV tests and two adapted ELISAs, which were evaluated and compared with a gold standard on blood. In addition a total IgG quantification was performed to demonstrate the quality of the specimen. HIV antibodies were detected with a sensitivity of 100%, 99.3%, 98.6%, 100% and 95.7% for, DPP, OraQuick, Aware, Genscreen and Vironostika respectively using the Intercept Collection Device. Respective specificities were 100%, 100%, 99.3%, 97.3% and 100%.

Original language	English
Journal	Journal of Virological Methods
Volume	234
Pages (from-to)	164-168
Number of pages	5
ISSN	0166-0934
DOIs	https://doi.org/10.1016/j.jviromet.2016.04.014
Publication status	Published - 2016

Keywords

Journal Article

Access to Document

10.1016/j.jviromet.2016.04.014Licence: Unspecified

Cite this

@article{4998bfa0bf194c4a9ff0c99b38795264,

title = "Evaluation of the intercept oral specimen collection device with HIV assays versus paired serum/plasma specimens",

abstract = "Oral fluid has many advantages over blood-based techniques: it is less invasive, eliminates the occupational risk associated with needle stick accidents and collection can be self-administrated. Each individual test is packaged with a corresponding collection device. This study tested the suitability of the Intercept Oral Specimen Collection Device for different HIV diagnostic tests: three different rapid HIV tests and two adapted ELISAs, which were evaluated and compared with a gold standard on blood. In addition a total IgG quantification was performed to demonstrate the quality of the specimen. HIV antibodies were detected with a sensitivity of 100%, 99.3%, 98.6%, 100% and 95.7% for, DPP, OraQuick, Aware, Genscreen and Vironostika respectively using the Intercept Collection Device. Respective specificities were 100%, 100%, 99.3%, 97.3% and 100%.",

keywords = "Journal Article",

author = "Greta Beelaert and {Van Heddegem}, L and {Van Frankenhuijsen}, M and G Vandewalle and V Compernolle and E Florence and K Fransen",

note = "NPP",

year = "2016",

doi = "10.1016/j.jviromet.2016.04.014",

language = "English",

volume = "234",

pages = "164--168",

journal = "Journal of Virological Methods",

issn = "0166-0934",

publisher = "Elsevier",

}

TY - JOUR

T1 - Evaluation of the intercept oral specimen collection device with HIV assays versus paired serum/plasma specimens

AU - Beelaert, Greta

AU - Van Heddegem, L

AU - Van Frankenhuijsen, M

AU - Vandewalle, G

AU - Compernolle, V

AU - Florence, E

AU - Fransen, K

N1 - NPP

PY - 2016

Y1 - 2016

N2 - Oral fluid has many advantages over blood-based techniques: it is less invasive, eliminates the occupational risk associated with needle stick accidents and collection can be self-administrated. Each individual test is packaged with a corresponding collection device. This study tested the suitability of the Intercept Oral Specimen Collection Device for different HIV diagnostic tests: three different rapid HIV tests and two adapted ELISAs, which were evaluated and compared with a gold standard on blood. In addition a total IgG quantification was performed to demonstrate the quality of the specimen. HIV antibodies were detected with a sensitivity of 100%, 99.3%, 98.6%, 100% and 95.7% for, DPP, OraQuick, Aware, Genscreen and Vironostika respectively using the Intercept Collection Device. Respective specificities were 100%, 100%, 99.3%, 97.3% and 100%.

AB - Oral fluid has many advantages over blood-based techniques: it is less invasive, eliminates the occupational risk associated with needle stick accidents and collection can be self-administrated. Each individual test is packaged with a corresponding collection device. This study tested the suitability of the Intercept Oral Specimen Collection Device for different HIV diagnostic tests: three different rapid HIV tests and two adapted ELISAs, which were evaluated and compared with a gold standard on blood. In addition a total IgG quantification was performed to demonstrate the quality of the specimen. HIV antibodies were detected with a sensitivity of 100%, 99.3%, 98.6%, 100% and 95.7% for, DPP, OraQuick, Aware, Genscreen and Vironostika respectively using the Intercept Collection Device. Respective specificities were 100%, 100%, 99.3%, 97.3% and 100%.

KW - Journal Article

U2 - 10.1016/j.jviromet.2016.04.014

DO - 10.1016/j.jviromet.2016.04.014

M3 - A1: Web of Science-article

C2 - 27142112

SN - 0166-0934

VL - 234

SP - 164

EP - 168

JO - Journal of Virological Methods

JF - Journal of Virological Methods

ER -

Evaluation of the intercept oral specimen collection device with HIV assays versus paired serum/plasma specimens

Abstract

Keywords

Access to Document

Fingerprint

Cite this