Evaluation of thin-layer agar 7H11 for the isolation of Mycobacterium tuberculosis complex

L Satti, A Ikram, S Abbasi, N Malik, IA Mirza, A Martin

    Research output: Contribution to journalA1: Web of Science-article


    We evaluated the thin-layer agar (TLA) method for the recovery of Mycobacterium tuberculosis complex and compared the results with the BACTEC Mycobacteria Growth Indicator Tube (MGIT) 960 system. A total of 53 mycobacterial isolates were isolated on both media. The recovery rates of mycobacteria on TLA and BACTEC MGIT 960 system were respectively 90.6% and 96.2%. Mean time to detection of mycobacteria on TLA was 12.5 compared to 11.2 days on BACTEC MGIT 960. TLA is a simple technique and can be used as an alternative to the Lowenstein-Jensen medium and BACTEC MGIT 960 for the isolation of mycobacteria in resource-poor settings
    Original languageEnglish
    JournalInternational Journal of Tuberculosis and Lung Disease
    Issue number10
    Pages (from-to)1354-1356
    Number of pages3
    Publication statusPublished - 2010


    • B780-tropical-medicine
    • Bacterial diseases
    • Tuberculosis
    • Mycobacterium tuberculosis complex
    • Detection
    • Evaluation
    • Isolation
    • Thin layer agar
    • BACTEC
    • Laboratory techniques and procedures


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