Evaluation of variant specific trypanolytic tests for serodiagnosis of human infections with Trypanosoma brucei gambiense

N Van Meirvenne, E Magnus, P Büscher

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Abstract

Twelve T.b. gambiense clone populations of distinct Variable Antigen Type (VAT) were combined in immune lysis tests with 340 sera of trypanosome infected patients from 8 different African countries and 267 non trypanosomiasis control sera. The diagnostic specificity of the test was 100%. At a serum dilution of 1:4 the overall test sensitivity with single VATs varied from 39.1 to 98.2% and from 12.1 to 86.8% at 1:32. At a serum dilution of 1:32 some combination tests with 2 VATs still scored above 96%. The VAT recognition patterns were clearly correlated with the geographical origin of the sera, reflecting a diversity in variable antigen repertoires.
Original languageEnglish
JournalActa Tropica
Volume60
Pages (from-to)189-199
ISSN0001-706X
Publication statusPublished - 1995

Keywords

  • B780-tropical-medicine
  • Protozoology
  • Trypanosomiasis
  • Trypanosoma brucei gambiense
  • Serodiagnosis
  • Trypanolysis
  • Laboratory medicine
  • Techniques
  • Variable antigens
  • Antibodies
  • Antigens
  • VSG
  • VAT

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