TY - JOUR
T1 - First insights into circulating Mycobacterium tuberculosis complex lineages and drug resistance in Guinea
AU - Ejo, Mebrat
AU - Gehre, Florian
AU - Barry, Mamadou Dian
AU - Sow, Oumou
AU - Bah, Nene Mamata
AU - Camara, Mory
AU - Bah, Boubacar
AU - Uwizeye, Cecile
AU - Nduwamahoro, Elie
AU - Fissette, Kristina
AU - De Rijk , P
AU - Merle, Corinne
AU - Olliaro, Piero
AU - Burgos, Marcos
AU - Lienhardt, Christian
AU - Rigouts, Leen
AU - de Jong, Bouke
N1 - Copyright © 2015. Published by Elsevier B.V.
PY - 2015
Y1 - 2015
N2 - In this study we assessed first-line anti-tuberculosis drug resistance and the genotypic distribution of Mycobacterium tuberculosis complex (MTBC) isolates that had been collected from consecutive new tuberculosis patients enrolled in two clinical trials conducted in Guinea between 2005 and 2010. Among the total 359 MTBC strains that were analyzed in this study, 22.8% were resistant to at least one of the first line anti-tuberculosis drugs, including 2.5% multidrug resistance and 17.5% isoniazid resistance, with or without other drugs. In addition, further characterization of isolates from a subset of the two trials (n=184) revealed a total of 80 different spoligotype patterns, 29 "orphan" and 51 shared patterns. We identified the six major MTBC lineages of human relevance, with predominance of the Euro-American lineage. In total, 132 (71.7%) of the strains were genotypically clustered, and further analysis (using the DESTUS model) suggesting significantly faster spread of LAM10_CAM family (p=0.00016). In conclusion, our findings provide a first insight into drug resistance and the population structure of the MTBC in Guinea, with relevance for public health scientists in tuberculosis control programs.
AB - In this study we assessed first-line anti-tuberculosis drug resistance and the genotypic distribution of Mycobacterium tuberculosis complex (MTBC) isolates that had been collected from consecutive new tuberculosis patients enrolled in two clinical trials conducted in Guinea between 2005 and 2010. Among the total 359 MTBC strains that were analyzed in this study, 22.8% were resistant to at least one of the first line anti-tuberculosis drugs, including 2.5% multidrug resistance and 17.5% isoniazid resistance, with or without other drugs. In addition, further characterization of isolates from a subset of the two trials (n=184) revealed a total of 80 different spoligotype patterns, 29 "orphan" and 51 shared patterns. We identified the six major MTBC lineages of human relevance, with predominance of the Euro-American lineage. In total, 132 (71.7%) of the strains were genotypically clustered, and further analysis (using the DESTUS model) suggesting significantly faster spread of LAM10_CAM family (p=0.00016). In conclusion, our findings provide a first insight into drug resistance and the population structure of the MTBC in Guinea, with relevance for public health scientists in tuberculosis control programs.
U2 - 10.1016/j.meegid.2015.05.022
DO - 10.1016/j.meegid.2015.05.022
M3 - A1: Web of Science-article
C2 - 26004194
SN - 1567-1348
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
ER -