First insights into circulating Mycobacterium tuberculosis complex lineages and drug resistance in Guinea

Mebrat Ejo, Florian Gehre, Mamadou Dian Barry, Oumou Sow, Nene Mamata Bah, Mory Camara, Boubacar Bah, Cecile Uwizeye, Elie Nduwamahoro, Kristina Fissette, P De Rijk , Corinne Merle, Piero Olliaro, Marcos Burgos, Christian Lienhardt, Leen Rigouts, Bouke de Jong

Research output: Contribution to journalA1: Web of Science-article

Abstract

In this study we assessed first-line anti-tuberculosis drug resistance and the genotypic distribution of Mycobacterium tuberculosis complex (MTBC) isolates that had been collected from consecutive new tuberculosis patients enrolled in two clinical trials conducted in Guinea between 2005 and 2010. Among the total 359 MTBC strains that were analyzed in this study, 22.8% were resistant to at least one of the first line anti-tuberculosis drugs, including 2.5% multidrug resistance and 17.5% isoniazid resistance, with or without other drugs. In addition, further characterization of isolates from a subset of the two trials (n=184) revealed a total of 80 different spoligotype patterns, 29 "orphan" and 51 shared patterns. We identified the six major MTBC lineages of human relevance, with predominance of the Euro-American lineage. In total, 132 (71.7%) of the strains were genotypically clustered, and further analysis (using the DESTUS model) suggesting significantly faster spread of LAM10_CAM family (p=0.00016). In conclusion, our findings provide a first insight into drug resistance and the population structure of the MTBC in Guinea, with relevance for public health scientists in tuberculosis control programs.

Original languageEnglish
JournalInfection, Genetics and Evolution
ISSN1567-1348
DOIs
Publication statusE-pub ahead of print - 2015

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