Functional analysis of the twin-arginine translocation pathway in Sodalis glossinidius, a bacterial symbiont of the tsetse fly

L De Vooght; G Caljon; M Coosemans; J Van Den Abbeele

doi:10.1128/AEM.02379-10

Functional analysis of the twin-arginine translocation pathway in Sodalis glossinidius, a bacterial symbiont of the tsetse fly

L De Vooght, G Caljon, M Coosemans, J Van Den Abbeele

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Abstract

This study demonstrates a functional twin-arginine (TAT) translocation pathway present in the tsetse fly symbiont Sodalis glossinidius and its potential to export active heterologous proteins to the periplasm. Functionality was demonstrated using green fluorescent protein (GFP) fused to the Tat signal peptide of E. coli trimethylamine N-oxide reductase (TorA).

Original language	English
Journal	Applied and Environmental Microbiology
Volume	77
Issue number	3
Pages (from-to)	1132-1134
Number of pages	3
ISSN	0099-2240
DOIs	https://doi.org/10.1128/AEM.02379-10
Publication status	Published - 2011

Keywords

B780-tropical-medicine
Protozoal diseases
Sleeping sickness
Trypanosomiasis-African
Trypanosoma brucei
Vectors
Tsetse flies
Glossina morsitans
Symbiosis
Bacteria
Sodalis glossinidius
Export
Proteins
Laboratory techniques and procedures

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10.1128/AEM.02379-10

2011aemi1132

http://lib.itg.be/pdf/itg/2011/2011aemi1132.pdf

Cite this

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title = "Functional analysis of the twin-arginine translocation pathway in Sodalis glossinidius, a bacterial symbiont of the tsetse fly",

abstract = "This study demonstrates a functional twin-arginine (TAT) translocation pathway present in the tsetse fly symbiont Sodalis glossinidius and its potential to export active heterologous proteins to the periplasm. Functionality was demonstrated using green fluorescent protein (GFP) fused to the Tat signal peptide of E. coli trimethylamine N-oxide reductase (TorA).",

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doi = "10.1128/AEM.02379-10",

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AU - Caljon, G

AU - Coosemans, M

AU - Van Den Abbeele, J

N1 - FTX; ITG-P1B; ITG-A2B; ITG-P3A; ITG-ALA; MULTI; PARAS; U-ENTOM; ANIMAL; U-VPROT; JIF; DOI; PDF; Abstract; DSPACE

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N2 - This study demonstrates a functional twin-arginine (TAT) translocation pathway present in the tsetse fly symbiont Sodalis glossinidius and its potential to export active heterologous proteins to the periplasm. Functionality was demonstrated using green fluorescent protein (GFP) fused to the Tat signal peptide of E. coli trimethylamine N-oxide reductase (TorA).

AB - This study demonstrates a functional twin-arginine (TAT) translocation pathway present in the tsetse fly symbiont Sodalis glossinidius and its potential to export active heterologous proteins to the periplasm. Functionality was demonstrated using green fluorescent protein (GFP) fused to the Tat signal peptide of E. coli trimethylamine N-oxide reductase (TorA).

KW - B780-tropical-medicine

KW - Protozoal diseases

KW - Sleeping sickness

KW - Trypanosomiasis-African

KW - Trypanosoma brucei

KW - Vectors

KW - Tsetse flies

KW - Glossina morsitans

KW - Symbiosis

KW - Bacteria

KW - Sodalis glossinidius

KW - Export

KW - Proteins

KW - Laboratory techniques and procedures

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DO - 10.1128/AEM.02379-10

M3 - A1: Web of Science-article

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SN - 0099-2240

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SP - 1132

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JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 3

ER -

Functional analysis of the twin-arginine translocation pathway in Sodalis glossinidius, a bacterial symbiont of the tsetse fly

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Keywords

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