Generation of a recombinant Gag virus-like-particle panel for the evaluation of p24 antigen detection by diagnostic HIV tests

Beatrice N Vetter, Vanessa Orlowski, Katrien Fransen, Christoph Niederhauser, Vincent Aubert, Marcel Brandenberger, Diana Ciardo, Günter Dollenmaier, Thomas Klimkait, Stephan Regenass, Patrick Schmid, Volkmar Schottstedt, Franziska Suter-Riniker, Sabine Yerly, Cyril Shah, Jürg Böni, Jörg Schüpbach

    Research output: Contribution to journalA1: Web of Science-article

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    Abstract

    BACKGROUND: Detection of HIV-1 p24 antigen permits early identification of primary HIV infection and timely intervention to limit further spread of the infection. Principally, HIV screening should equally detect all viral variants, but reagents for a standardised test evaluation are limited. Therefore, we aimed to create an inexhaustible panel of diverse HIV-1 p24 antigens.

    METHODS: We generated a panel of 43 recombinantly expressed virus-like particles (VLPs), containing the structural Gag proteins of HIV-1 subtypes A-H and circulating recombinant forms (CRF) CRF01_AE, CRF02_AG, CRF12_BF, CRF20_BG and group O. Eleven 4th generation antigen/antibody tests and five antigen-only tests were evaluated for their ability to detect VLPs diluted in human plasma to p24 concentrations equivalent to 50, 10 and 2 IU/ml of the WHO p24 standard. Three tests were also evaluated for their ability to detect p24 after heat-denaturation for immune-complex disruption, a pre-requisite for ultrasensitive p24 detection.

    RESULTS: Our VLP panel exhibited an average intra-clade p24 diversity of 6.7%. Among the 4th generation tests, the Abbott Architect and Siemens Enzygnost Integral 4 had the highest sensitivity of 97.7% and 93%, respectively. Alere Determine Combo and BioRad Access were least sensitive with 10.1% and 40.3%, respectively. Antigen-only tests were slightly more sensitive than combination tests. Almost all tests detected the WHO HIV-1 p24 standard at a concentration of 2 IU/ml, but their ability to detect this input for different subtypes varied greatly. Heat-treatment lowered overall detectability of HIV-1 p24 in two of the three tests, but only few VLPs had a more than 3-fold loss in p24 detection.

    CONCLUSIONS: The HIV-1 Gag subtype panel has a broad diversity and proved useful for a standardised evaluation of the detection limit and breadth of subtype detection of p24 antigen-detecting tests. Several tests exhibited problems, particularly with non-B subtypes.

    Original languageEnglish
    JournalPLoS ONE
    Volume9
    Issue number10
    Pages (from-to)e111552
    ISSN1932-6203
    DOIs
    Publication statusPublished - 2014

    Keywords

    • Amino Acid Sequence
    • HIV Antigens
    • HIV Core Protein p24
    • HIV Infections
    • Humans
    • Molecular Sequence Data
    • Phylogeny
    • Protein Denaturation
    • Reagent Kits, Diagnostic
    • Recombinant Proteins
    • Virion
    • gag Gene Products, Human Immunodeficiency Virus

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