Genetic diversity of Mycobacterium tuberculosis from Pará, Brazil, reveals a higher frequency of ancestral strains than previously reported in South America

Emilyn Costa Conceicao, Nalin Rastogi, David Couvin, Maria Luiza Lopes, Ismari Perini Furlaneto, Harrison Magdinier Gomes, Sidra Ezidio Goncalves Vasconcellos, Philip Noel Suffys, Maria Paula Cruz Schneider, Maiaa Silva de Sousa, Christophe Sola, Ricardo Jose de Paula Souza e Guimaraes, Rafael Silva Duarte, Karla Valeria Batista Lima

Research output: Contribution to journalA1: Web of Science-article

Abstract

There is only scarce information available on genotypic diversity of the Mycobacterium tuberculosis complex (MTBC) clinical isolates circulating in the Northern part of Brazil, a relatively neglected region regarding research on tuberculosis. We therefore characterized 980 MTBC clinical isolates from the state of Para, by spoligotyping and data was compared with patterns from around the world, besides analyzing drug susceptibility, and collecting sociodemographic data. We also performed 24 loci MIRU-VNTR typing to evaluate phylogenetic inferences among the East-African-Indian (EAI) lineage strains. The Geographic Information System analyses were performed to generate a descriptive visualization of MTBC strain distribution in the region. A total of 249 different spoligopatterns primarily belonging to evolutionary recent Euro-American lineages, as well as CentralAsian, Manu and ancestral EAI lineages, were identified, in addition to strains with reportedly unknown lineage signatures. The most frequent lineages were Latin American Mediterranean, T and Haarlem. Interestingly, EAI lineage strains were found in a significantly higher proportion in comparison with previous studies from South America. Regarding EAI lineage, the absence of spacers 4-9 and 23-24 co-related to 24 loci MIRU-VNTRs may suggest a close evolutionary relationship between such strains in Para and those prevalent in Mozambique, which might have contributed to the genetic diversity of MTBC strains in this region.

Original languageEnglish
JournalInfection, Genetics and Evolution
Volume56
Pages (from-to)62-72
Number of pages11
ISSN1567-1348
DOIs
Publication statusPublished - 2017

Keywords

  • Mycobacterium tuberculosis
  • Population-structure
  • Spoligotyping
  • Brazil
  • Euro-American lineage
  • EAI lineage
  • MOLECULAR EPIDEMIOLOGY
  • POPULATION-STRUCTURE
  • MINAS-GERAIS
  • WEB TOOL
  • STATE
  • COMPLEX
  • RDRIO
  • FAMILY
  • SPOLIGOTYPES
  • RESISTANCE

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