Genetic markers for SSG resistance in Leishmania donovani and SSG treatment failure in visceral leishmaniasis patients of the Indian subcontinent

M. Vanaerschot; S. Decuypere; T. Downing; H. Imamura; O. Stark; S. De Doncker; S. Roy; B. Ostyn; L. Maes; B. Khanal; M. Boelaert; G. Schönian; M. Berriman; F. Chappuis; J.C. Dujardin; S. Sundar; S. Rijal

doi:10.1093/infdis/jis424

Genetic markers for SSG resistance in Leishmania donovani and SSG treatment failure in visceral leishmaniasis patients of the Indian subcontinent

M. Vanaerschot, S. Decuypere, T. Downing, H. Imamura, O. Stark, S. De Doncker, S. Roy, B. Ostyn, L. Maes, B. Khanal, M. Boelaert, G. Schönian, M. Berriman, F. Chappuis, J.C. Dujardin, S. Sundar, S. Rijal

Molecular Parasitology

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

The current standard to assess pentavalent antimonial (SSG) susceptibility of Leishmania is a laborious in vitro assay of which the result has little clinical value because SSG-resistant parasites are also found in SSG-cured patients. Candidate genetic markers for clinically relevant SSG-resistant parasites identified by full genome sequencing were here validated on a larger set of clinical strains. We show that 3 genomic locations suffice to specifically detect the SSG-resistant parasites found only in patients experiencing SSG treatment failure. This finding allows the development of rapid assays to monitor the emergence and spread of clinically relevant SSG-resistant Leishmania parasites.

Original language	English
Journal	Journal of Infectious Diseases
Volume	206
Issue number	5
Pages (from-to)	752-755
Number of pages	4
ISSN	0022-1899
DOIs	https://doi.org/10.1093/infdis/jis424
Publication status	Published - 2012

Keywords

Protozoal diseases
Visceral
Leishmaniasis
Kala azar
Leishmania donovani
Vectors
Sandflies
Phlebotomus argentipes
Drug resistance
SSG
Antimonials
Treatment failure
Genetic markers
Genome sequencing
Strains
Haplotypes
Identification
Assays
India
Asia-South

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10.1093/infdis/jis424

http://lib.itg.be/pdf/itg/2012/2012jidi0752.pdf

Cite this

Vanaerschot, M., Decuypere, S., Downing, T., Imamura, H., Stark, O., De Doncker, S., Roy, S., Ostyn, B., Maes, L., Khanal, B., Boelaert, M., Schönian, G., Berriman, M., Chappuis, F., Dujardin, J. C., Sundar, S., & Rijal, S. (2012). Genetic markers for SSG resistance in Leishmania donovani and SSG treatment failure in visceral leishmaniasis patients of the Indian subcontinent. Journal of Infectious Diseases, 206(5), 752-755. https://doi.org/10.1093/infdis/jis424

Vanaerschot, M. ; Decuypere, S. ; Downing, T. ; Imamura, H. ; Stark, O. ; De Doncker, S. ; Roy, S. ; Ostyn, B. ; Maes, L. ; Khanal, B. ; Boelaert, M. ; Schönian, G. ; Berriman, M. ; Chappuis, F. ; Dujardin, J.C. ; Sundar, S. ; Rijal, S. / Genetic markers for SSG resistance in Leishmania donovani and SSG treatment failure in visceral leishmaniasis patients of the Indian subcontinent. In: Journal of Infectious Diseases. 2012 ; Vol. 206, No. 5. pp. 752-755.

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title = "Genetic markers for SSG resistance in Leishmania donovani and SSG treatment failure in visceral leishmaniasis patients of the Indian subcontinent",

abstract = "The current standard to assess pentavalent antimonial (SSG) susceptibility of Leishmania is a laborious in vitro assay of which the result has little clinical value because SSG-resistant parasites are also found in SSG-cured patients. Candidate genetic markers for clinically relevant SSG-resistant parasites identified by full genome sequencing were here validated on a larger set of clinical strains. We show that 3 genomic locations suffice to specifically detect the SSG-resistant parasites found only in patients experiencing SSG treatment failure. This finding allows the development of rapid assays to monitor the emergence and spread of clinically relevant SSG-resistant Leishmania parasites.",

keywords = "Protozoal diseases, Visceral, Leishmaniasis, Kala azar, Leishmania donovani, Vectors, Sandflies, Phlebotomus argentipes, Drug resistance, SSG, Antimonials, Treatment failure, Genetic markers, Genome sequencing, Strains, Haplotypes, Identification, Assays, India, Asia-South",

author = "M. Vanaerschot and S. Decuypere and T. Downing and H. Imamura and O. Stark and {De Doncker}, S. and S. Roy and B. Ostyn and L. Maes and B. Khanal and M. Boelaert and G. Sch{\"o}nian and M. Berriman and F. Chappuis and J.C. Dujardin and S. Sundar and S. Rijal",

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doi = "10.1093/infdis/jis424",

language = "English",

volume = "206",

pages = "752--755",

journal = "Journal of Infectious Diseases",

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Vanaerschot, M, Decuypere, S, Downing, T, Imamura, H, Stark, O, De Doncker, S, Roy, S, Ostyn, B, Maes, L, Khanal, B, Boelaert, M, Schönian, G, Berriman, M, Chappuis, F, Dujardin, JC, Sundar, S & Rijal, S 2012, 'Genetic markers for SSG resistance in Leishmania donovani and SSG treatment failure in visceral leishmaniasis patients of the Indian subcontinent', Journal of Infectious Diseases, vol. 206, no. 5, pp. 752-755. https://doi.org/10.1093/infdis/jis424

Genetic markers for SSG resistance in Leishmania donovani and SSG treatment failure in visceral leishmaniasis patients of the Indian subcontinent. / Vanaerschot, M.; Decuypere, S.; Downing, T.; Imamura, H.; Stark, O.; De Doncker, S.; Roy, S.; Ostyn, B.; Maes, L.; Khanal, B.; Boelaert, M.; Schönian, G.; Berriman, M.; Chappuis, F.; Dujardin, J.C.; Sundar, S.; Rijal, S.

In: Journal of Infectious Diseases, Vol. 206, No. 5, 2012, p. 752-755.

Research output: Contribution to journal › A1: Web of Science-article › peer-review

TY - JOUR

T1 - Genetic markers for SSG resistance in Leishmania donovani and SSG treatment failure in visceral leishmaniasis patients of the Indian subcontinent

AU - Vanaerschot, M.

AU - Decuypere, S.

AU - Downing, T.

AU - Imamura, H.

AU - Stark, O.

AU - De Doncker, S.

AU - Roy, S.

AU - Ostyn, B.

AU - Maes, L.

AU - Khanal, B.

AU - Boelaert, M.

AU - Schönian, G.

AU - Berriman, M.

AU - Chappuis, F.

AU - Dujardin, J.C.

AU - Sundar, S.

AU - Rijal, S.

N1 - PPU; ITG-B1A; ITG-B2B; ITG-B4B; ITG-B6B; ITG-H8B; ITG-H11A; ITG-B15A; MULTI; DBM; U-MOLPAR; DPH; U-ECTD; JIF; DOI; PDF; Abstract; DSPACE

PY - 2012

Y1 - 2012

N2 - The current standard to assess pentavalent antimonial (SSG) susceptibility of Leishmania is a laborious in vitro assay of which the result has little clinical value because SSG-resistant parasites are also found in SSG-cured patients. Candidate genetic markers for clinically relevant SSG-resistant parasites identified by full genome sequencing were here validated on a larger set of clinical strains. We show that 3 genomic locations suffice to specifically detect the SSG-resistant parasites found only in patients experiencing SSG treatment failure. This finding allows the development of rapid assays to monitor the emergence and spread of clinically relevant SSG-resistant Leishmania parasites.

AB - The current standard to assess pentavalent antimonial (SSG) susceptibility of Leishmania is a laborious in vitro assay of which the result has little clinical value because SSG-resistant parasites are also found in SSG-cured patients. Candidate genetic markers for clinically relevant SSG-resistant parasites identified by full genome sequencing were here validated on a larger set of clinical strains. We show that 3 genomic locations suffice to specifically detect the SSG-resistant parasites found only in patients experiencing SSG treatment failure. This finding allows the development of rapid assays to monitor the emergence and spread of clinically relevant SSG-resistant Leishmania parasites.

KW - Protozoal diseases

KW - Visceral

KW - Leishmaniasis

KW - Kala azar

KW - Leishmania donovani

KW - Vectors

KW - Sandflies

KW - Phlebotomus argentipes

KW - Drug resistance

KW - SSG

KW - Antimonials

KW - Treatment failure

KW - Genetic markers

KW - Genome sequencing

KW - Strains

KW - Haplotypes

KW - Identification

KW - Assays

KW - India

KW - Asia-South

U2 - 10.1093/infdis/jis424

DO - 10.1093/infdis/jis424

M3 - A1: Web of Science-article

C2 - 22753945

VL - 206

SP - 752

EP - 755

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 5

ER -

Genetic markers for SSG resistance in Leishmania donovani and SSG treatment failure in visceral leishmaniasis patients of the Indian subcontinent

Abstract

Keywords

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