Abstract
BACKGROUND: The aim of this study was to investigate the genetic diversity among Mycobacterium tuberculosis complex circulating in patients with no known risk factors for multi-drug resistant (MDR) tuberculosis (TB) living in a high MDR burden area and analyze the relationship between genotypes, primary drug resistance and age. METHODS: Samples were collected during January-July 2009. Isolates were tested for drug susceptibility to first-line drugs and were genotyped by spoligotyping and the 15-loci Mycobacterial Interspersed Repetitive Unit. RESULTS: Among the 199 isolates analyzed, 169 (84.9%) were identified in the SpolDB4.0 and 30 (15.1%) could not be matched to any lineage. The most prevalent lineage was Haarlem (29.6%), followed by T (15.6%), Beijing (14.1%), Latin American Mediterranean (12.6%) and U (8.5%). A few isolates belonged to the X and S clades (4.5%). Spoligotype analysis identified clustering among 148 of 169 isolates, whereas with MIRU15 all isolates were unique. Out of 197 strains; 31.5% were resistant to at least one drug, 7.5% were MDR and 22.3% showed any resistance to isoniazid. CONCLUSION: In contrast with other Latin-American countries where LAM lineage is the most predominant, we found the spoligotype 50 from the Haarlem lineage as the most common. None of the prevailing lineages showed a significant association with age or resistance to isoniazid and/or rifampicin.
Original language | English |
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Journal | BMC Infectious Diseases |
Volume | 13 |
Pages (from-to) | 397 |
Number of pages | 7 |
ISSN | 1471-2334 |
DOIs | |
Publication status | Published - 2013 |
Keywords
- Bacterial diseases
- Tuberculosis
- Mycobacterium tuberculosis
- Molecular epidemiology
- Genetic variability
- Genetic diversity
- Risk factors
- Genotypes
- Primary
- Drug resistance
- Isoniazid
- Rifampicin
- Age
- Identification
- Strains
- Clustering
- Peru
- America-Latin