Genomic heterogeneity of AIDS retroviral isolates from North America and Zaire

S Benn, R Rutledge, T Folks, J Gold, L Baker, J McCormick, P Feorino, P Piot, T Quinn, M Martin

Research output: Contribution to journalA2: International peer reviewed article (not A1-type)

Abstract

In an analysis of the genomic variation of AIDS retroviral isolates from patients living in New York, Alabama, and Zaire, restriction maps were constructed by using seven enzymes, each known to cleave the proviral DNA more than once, in conjunction with Southern blot analysis. The maps of LAV, HTLV-III, and ARV-2 as deduced from their published nucleotide sequences were included in this analysis. The results demonstrated that (i) several 'signature' restriction sites were common to all isolates; (ii) with the exception of LAV and HTLV-III, the North American and European isolates were all different from one another and showed no geographical specificity; (iii) the African isolates as a group were more diverse than those from North America and Europe; and (iv) the genomic variability was concentrated within the env gene
Original languageEnglish
JournalScience
Volume230
Pages (from-to)949-951
ISSN0036-8075
Publication statusPublished - 1985

Keywords

  • B780-tropical-medicine
  • AIDS
  • Viral diseases
  • Virology
  • HTLV
  • Genomes
  • Base sequence
  • Chromosome mapping
  • Cloning
  • DNA
  • Diagnostic use
  • America-North
  • Congo-Kinshasa
  • Africa-Central

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