TY - JOUR
T1 - Growth and cytotoxic activity by Mycobacterium ulcerans in protein-free media
AU - Mve-Obiang, A
AU - Remacle, J
AU - Palomino, JC
AU - Houbion, A
AU - Portaels, F
N1 - FTX: Abonnement
PY - 1999
Y1 - 1999
N2 - The pathogenic slow-growing Mycobacterium ulcerans has, until now, been cultured in liquid media containing albumin. Here, we report the first description of use of Sauton medium and modified Reid medium, two protein-free media, in which M. ulcerans was able to grow and produce its toxin, a major virulence factor of this environmental organism which causes a skin disease commonly called Buruli ulcer. These results suggest that Sauton and modified Reid may be useful for certain fields of M. ulcerans research requiring protein-free growth conditions.
AB - The pathogenic slow-growing Mycobacterium ulcerans has, until now, been cultured in liquid media containing albumin. Here, we report the first description of use of Sauton medium and modified Reid medium, two protein-free media, in which M. ulcerans was able to grow and produce its toxin, a major virulence factor of this environmental organism which causes a skin disease commonly called Buruli ulcer. These results suggest that Sauton and modified Reid may be useful for certain fields of M. ulcerans research requiring protein-free growth conditions.
KW - B780-tropical-medicine
KW - Bacterial diseases
KW - Mycobacterium ulcerans
KW - Experimental
UR - https://www.webofscience.com/wos/woscc/full-record/WOS:000083844400020
U2 - 10.1016/S0378-1097(99)00528-5
DO - 10.1016/S0378-1097(99)00528-5
M3 - A1: Web of Science-article
SN - 0378-1097
VL - 181
SP - 153
EP - 157
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
ER -