Growth of gram-negative bacteria in antiseptics, disinfectants and hand hygiene products in two tertiary care hospitals in West Africa-a cross-sectional survey

Palpouguini Lompo, Anne-Sophie Heroes, Esenam Agbobli, Adama Kazienga, Marjan Peeters, Halidou Tinto, Katrien Lagrou, Lassana Sangaré, Dissou Affolabi, Jan Jacobs

Research output: Contribution to journalA1: Web of Science-articlepeer-review

39 Downloads (Pure)

Abstract

Antiseptics, disinfectants, and hand hygiene products can act as reservoirs of Gram-negative bacteria causing healthcare-associated infections. This problem is rarely documented in low- and middle-income countries, particularly in sub-Saharan Africa. In a cross-sectional survey, we assessed the bacterial contamination of antiseptics, disinfectants, and hand hygiene products in two university hospitals in Burkina Faso and Benin. During ward visits and staff interviews, in-use products were cultured for the presence of Gram-negative bacteria. The growth of Gram-negative bacteria was absent or rare in alcohol-based products, povidone iodine, and Dakin solution. Contamination was highest (73.9% (51/69)) for liquid soap products (versus antiseptic/disinfectants (4.5%, 7/157) (p < 0.0001)), mostly used in high-risk areas and associated with high total bacterial counts (>10,000 colony-forming units/mL). Contaminating flora (105 isolates) included Enterobacterales and the Vibrio non-cholerae/Aeromonas group (17.1%) and non-fermentative Gram-negative rods (82.8%). Multidrug resistance was present among 9/16 Enterobacterales (Klebsiella and Enterobacter spp.) and 3/12 Acinetobacter spp., including carbapenem resistance (Acinetobacter baumannii: NDM, Pseudomonas stutzeri: VIM). The risk factors for contamination included the type of product (cleaning grade and in-house prepared liquid soap), use of recycled disposable containers and soft drink bottles, absence of labeling, topping-up of containers, dilution with tap water (pharmacy and ward), and poor-quality management (procurement, stock management, expiry dates, and period after opening).

Original languageEnglish
Article number917
JournalPathogens
Volume12
Issue number7
ISSN2076-0817
DOIs
Publication statusPublished - 2023

Fingerprint

Dive into the research topics of 'Growth of gram-negative bacteria in antiseptics, disinfectants and hand hygiene products in two tertiary care hospitals in West Africa-a cross-sectional survey'. Together they form a unique fingerprint.

Cite this