Hematopoietic stem/progenitor cell sources to generate reticulocytes for Plasmodium vivax culture

Florian Noulin, Javed Karim Manesia, Anna Rosanas-Urgell, Annette Erhart, Céline Borlon, Jakke Van Den Abbeele, Umberto d'Alessandro, Catherine M Verfaillie

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Abstract

The predilection of Plasmodium vivax (P. vivax) for reticulocytes is a major obstacle for its establishment in a long-term culture system, as this requires a continuous supply of large quantities of reticulocytes, representing only 1-2% of circulating red blood cells. We here compared the production of reticulocytes using an established in vitro culture system from three different sources of hematopoietic stem/progenitor cells (HSPC), i.e. umbilical cord blood (UCB), bone marrow (BM) and adult peripheral blood (PB). Compared to CD34+-enriched populations of PB and BM, CD34+-enriched populations of UCB produced the highest amount of reticulocytes that could be invaded by P. vivax. In addition, when CD34+-enriched cells were first expanded, a further extensive increase in reticulocytes was seen for UCB, to a lesser degree BM but not PB. As invasion by P. vivax was significantly better in reticulocytes generated in vitro, we also suggest that P. vivax may have a preference for invading immature reticulocytes, which should be confirmed in future studies.

Original languageEnglish
JournalPLoS ONE
Volume9
Issue number11
Pages (from-to)e112496
ISSN1932-6203
DOIs
Publication statusPublished - 2014

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