High-throughput analysis of the Trypanosoma cruzi minicirculome (mcDNA) unveils structural variation and functional diversity

A Gómez-Palacio, L Cruz-Saavedra, F van den Broeck, M Geerts, S Pita, GA Vallejo, JC Carranza, JD Ramírez

    Research output: Contribution to journalA1: Web of Science-articlepeer-review

    Abstract

    Trypanosoma cruzi causes Chagas disease and has a unique extranuclear genome enclosed in a structure called the kinetoplast, which contains circular genomes known as maxi- and minicircles. While the structure and function of maxicircles are well-understood, many aspects of minicircles remain to be discovered. Here, we performed a high-throughput analysis of the minicirculome (mcDNA) in 50 clones isolated from Colombia’s diverse T. cruzi I populations. Results indicate that mcDNA comprises four diverse subpopulations with different structures, lengths, and numbers of interspersed semi-conserved (previously termed ultra-conserved regions mHCV) and hypervariable (mHVPs) regions. Analysis of mcDNA ancestry and inter-clone differentiation indicates the interbreeding of minicircle sequence classes is placed along diverse strains and hosts. These results support evidence of the multiclonal dynamics and random bi-parental segregation. Finally, we disclosed the guide RNA repertoire encoded by mcDNA at a clonal scale, and several attributes of its abundance and function are discussed.
    Original languageEnglish
    Article number5578
    JournalScientific Reports
    Volume14
    Issue number1
    Number of pages14
    ISSN2045-2322
    DOIs
    Publication statusPublished - 2024

    Keywords

    • Chagas disease
    • Colombia
    • Guide RNA
    • Maxicircles
    • Minicircles
    • Trypanosoma cruzi
    • KDNA

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