HIV-1 infection impairs CD16 and CD35 mediated opsonophagocytosis of Mycobacterium tuberculosis by human neutrophils

Nonzwakazi Bangani, Justine Nakiwala, Adrian R. Martineau, Robert J. Wilkinson, Katalin A. Wilkinson, David M. Lowe

Research output: Contribution to journalA1: Web of Science-article

Abstract

Using a flow cytometric assay, we investigated neutrophil-Mycobacterium tuberculosis opsonophagocytosis and the impact of HIV-1-infected serum on this process. The mean (+/- SD) percentage of neutrophils internalizing bacilli after 30 minutes incubation was significantly reduced by pretreatment with anti-CD16 (18.2% +/- 8.1%, P <0.001) or anti-CD35 antibody (23.2% +/- 10.6%, P <0.05) versus anti-CD4 controls (29.9% +/- 8.1%). Blocking CD88 or CD11a did not affect internalization. Using heat-inactivated serum, maximal internalization was lower using HIV-1-infected serum versus HIV-1-uninfected. Using non-heat-inactivated serum, internalization decreased more rapidly with sequential dilutions of HIV-1-infected versus HIV-1uninfected serum. CD16 and CD35 are important for neutrophil internalization of M. tuberculosis, whereas HIV-1 infection adversely affects opsonophagocytosis.

Original languageEnglish
JournalJournal of Acquired Immune Deficiency Syndromes
Volume73
Issue number3
Pages (from-to)263-267
Number of pages5
ISSN1525-4135
Publication statusPublished - 2016

Keywords

  • neutrophil
  • phagocytosis
  • opsonization
  • tuberculosis
  • mycobacteria
  • HIV
  • ANTIBODIES
  • PHAGOCYTOSIS
  • VACCINATION
  • INNATE
  • CELLS
  • ASSAY

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