Abstract
Using a flow cytometric assay, we investigated neutrophil-Mycobacterium tuberculosis opsonophagocytosis and the impact of HIV-1-infected serum on this process. The mean (+/- SD) percentage of neutrophils internalizing bacilli after 30 minutes incubation was significantly reduced by pretreatment with anti-CD16 (18.2% +/- 8.1%, P <0.001) or anti-CD35 antibody (23.2% +/- 10.6%, P <0.05) versus anti-CD4 controls (29.9% +/- 8.1%). Blocking CD88 or CD11a did not affect internalization. Using heat-inactivated serum, maximal internalization was lower using HIV-1-infected serum versus HIV-1-uninfected. Using non-heat-inactivated serum, internalization decreased more rapidly with sequential dilutions of HIV-1-infected versus HIV-1uninfected serum. CD16 and CD35 are important for neutrophil internalization of M. tuberculosis, whereas HIV-1 infection adversely affects opsonophagocytosis.
| Original language | English |
|---|---|
| Journal | Journal of Acquired Immune Deficiency Syndromes |
| Volume | 73 |
| Issue number | 3 |
| Pages (from-to) | 263-267 |
| Number of pages | 5 |
| ISSN | 1525-4135 |
| Publication status | Published - 2016 |
Keywords
- neutrophil
- phagocytosis
- opsonization
- tuberculosis
- mycobacteria
- HIV
- ANTIBODIES
- PHAGOCYTOSIS
- VACCINATION
- INNATE
- CELLS
- ASSAY