hsp65 PCR-restriction analysis (PRA) with capillary electrophoresis for species identification and differentiation of Mycobacterium kansasii and Mycobacterium chelonae-Mycobacterium abscessus group

A Sajduda, A. Martin, F Portaels, J.C. Palomino

Research output: Contribution to journalA1: Web of Science-article

Abstract

OBJECTIVES: The aim of the present study was to identify and differentiate Mycobacterium kansasii and Mycobacterium chelonae-Mycobacterium abscessus group strains isolated from clinical and environmental sources in different countries. METHODS: PCR-restriction analysis of the hsp65 gene (PRA) with automated capillary electrophoresis was applied to the isolates previously identified by conventional biochemical testing and the molecular INNO-LiPA MYCOBACTERIA assay. RESULTS: PRA performed very well in comparison with the two other methods (96.4% concordance). Among 27M. kansasii isolates, this method detected five genetic types, of which type 1 represented the most common clinical isolate, as it is worldwide. PRA differentiated 29M. chelonae-M. abscessus group isolates into Mycobacterium immunogenum type 2 (n=13), M. chelonae (n=12), and M. abscessus types 1 (n=1) and 2 (n=1). M. immunogenum was the most frequent (69%) isolate from humans, but only one of 11 cases was clinically significant. M. chelonae was the most commonly (83%) recovered from water. PRA also identified two isolates with hsp65 alleles representing previously unreported patterns. CONCLUSIONS: PRA based on automated capillary electrophoresis is a rapid, simple, and reliable method for the identification and differentiation of both clinically relevant and environmental isolates of M. kansasii and M. chelonae-M. abscessus group.
Original languageEnglish
JournalInternational Journal of Infectious Diseases
Volume16
Issue number3
Pages (from-to)e193-e197
ISSN1201-9712
DOIs
Publication statusPublished - 2012

Keywords

  • B780-tropical-medicine
  • Bacterial diseases
  • Tuberculosis
  • Leprosy
  • Mycobacterium kansasii
  • Mycobacterium chelonae
  • Mycobacterium abscessus
  • Molecular diagnostic techniques
  • Species
  • Identification
  • Differentiation
  • Polymerase chain reaction
  • PCR-restriction analysis
  • Electrophoresis
  • Laboratory techniques and procedures

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