Identification of a functional Antigen5-related allergen in the saliva of a blood feeding insect, the tsetse fly

G Caljon; K Broos; I De Goeyse; K De Ridder; JM Sternberg; M Coosemans; P De Baetselier; Y Guisez; J Van Den Abbeele

doi:10.1016/j.ibmb.2009.01.010

Identification of a functional Antigen5-related allergen in the saliva of a blood feeding insect, the tsetse fly

G Caljon
, K Broos
, I De Goeyse
, K De Ridder
, JM Sternberg
, M Coosemans
, P De Baetselier
, Y Guisez
, J Van Den Abbeele

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

Our previous screening of a Glossma morsitans; morsitans lambda gt11 salivary gland expression library with serum of a tsetse fly exposed rabbit identified a cDNA encoding Tsetse Antigen5 (TAg5, 28.9 kDa), a homologue of Antigen5 sting venom allergens. Recombinant TAg5 was produced in Sf9 cells in order to assess its immunogenic properties in humans. Plasma from a patient that previously exhibited anaphylactic reactions against tsetse fly bites contained circulating anti-TAg5 and anti-saliva IgEs. In a significant proportion of plasma samples of African individuals, TAg5 and saliva binding IgEs (respectively 56 and 65%) can be detected. Saliva, harvested from flies that were subjected to TAg5-specific RNA interference (RNAi), displayed significantly reduced IgE binding potential. Allergenic properties of TAg5 and tsetse fly saliva were further illustrated in immunized mice, using an immediate cutaneous hypersensitivity and passive cutaneous anaphylaxis assay. Collectively, TAg5 was illustrated to be a tsetse fly salivary allergen, demonstrating that Antigen5-related proteins are represented as functional allergens not only in stinging but also in blood feeding insects.

Original language	English
Journal	Insect Biochemistry and Molecular Biology
Volume	39
Issue number	5-6
Pages (from-to)	332-341
Number of pages	10
ISSN	0965-1748
DOIs	https://doi.org/10.1016/j.ibmb.2009.01.010
Publication status	Published - 2009

Keywords

B780-tropical-medicine
Entomology
Tsetse flies
Glossina morsitans morsitans
Saliva
Allergen
TAg5
Antigen 5
Antibodies
Immunoglobulin E
Hypersensitivity

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10.1016/j.ibmb.2009.01.010

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title = "Identification of a functional Antigen5-related allergen in the saliva of a blood feeding insect, the tsetse fly",

abstract = "Our previous screening of a Glossma morsitans; morsitans lambda gt11 salivary gland expression library with serum of a tsetse fly exposed rabbit identified a cDNA encoding Tsetse Antigen5 (TAg5, 28.9 kDa), a homologue of Antigen5 sting venom allergens. Recombinant TAg5 was produced in Sf9 cells in order to assess its immunogenic properties in humans. Plasma from a patient that previously exhibited anaphylactic reactions against tsetse fly bites contained circulating anti-TAg5 and anti-saliva IgEs. In a significant proportion of plasma samples of African individuals, TAg5 and saliva binding IgEs (respectively 56 and 65\%) can be detected. Saliva, harvested from flies that were subjected to TAg5-specific RNA interference (RNAi), displayed significantly reduced IgE binding potential. Allergenic properties of TAg5 and tsetse fly saliva were further illustrated in immunized mice, using an immediate cutaneous hypersensitivity and passive cutaneous anaphylaxis assay. Collectively, TAg5 was illustrated to be a tsetse fly salivary allergen, demonstrating that Antigen5-related proteins are represented as functional allergens not only in stinging but also in blood feeding insects.",

keywords = "B780-tropical-medicine, Entomology, Tsetse flies, Glossina morsitans morsitans, Saliva, Allergen, TAg5, Antigen 5, Antibodies, Immunoglobulin E, Hypersensitivity",

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T1 - Identification of a functional Antigen5-related allergen in the saliva of a blood feeding insect, the tsetse fly

AU - Caljon, G

AU - Broos, K

AU - De Goeyse, I

AU - De Ridder, K

AU - Sternberg, JM

AU - Coosemans, M

AU - De Baetselier, P

AU - Guisez, Y

AU - Van Den Abbeele, J

N1 - FTX abonnement

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N2 - Our previous screening of a Glossma morsitans; morsitans lambda gt11 salivary gland expression library with serum of a tsetse fly exposed rabbit identified a cDNA encoding Tsetse Antigen5 (TAg5, 28.9 kDa), a homologue of Antigen5 sting venom allergens. Recombinant TAg5 was produced in Sf9 cells in order to assess its immunogenic properties in humans. Plasma from a patient that previously exhibited anaphylactic reactions against tsetse fly bites contained circulating anti-TAg5 and anti-saliva IgEs. In a significant proportion of plasma samples of African individuals, TAg5 and saliva binding IgEs (respectively 56 and 65%) can be detected. Saliva, harvested from flies that were subjected to TAg5-specific RNA interference (RNAi), displayed significantly reduced IgE binding potential. Allergenic properties of TAg5 and tsetse fly saliva were further illustrated in immunized mice, using an immediate cutaneous hypersensitivity and passive cutaneous anaphylaxis assay. Collectively, TAg5 was illustrated to be a tsetse fly salivary allergen, demonstrating that Antigen5-related proteins are represented as functional allergens not only in stinging but also in blood feeding insects.

AB - Our previous screening of a Glossma morsitans; morsitans lambda gt11 salivary gland expression library with serum of a tsetse fly exposed rabbit identified a cDNA encoding Tsetse Antigen5 (TAg5, 28.9 kDa), a homologue of Antigen5 sting venom allergens. Recombinant TAg5 was produced in Sf9 cells in order to assess its immunogenic properties in humans. Plasma from a patient that previously exhibited anaphylactic reactions against tsetse fly bites contained circulating anti-TAg5 and anti-saliva IgEs. In a significant proportion of plasma samples of African individuals, TAg5 and saliva binding IgEs (respectively 56 and 65%) can be detected. Saliva, harvested from flies that were subjected to TAg5-specific RNA interference (RNAi), displayed significantly reduced IgE binding potential. Allergenic properties of TAg5 and tsetse fly saliva were further illustrated in immunized mice, using an immediate cutaneous hypersensitivity and passive cutaneous anaphylaxis assay. Collectively, TAg5 was illustrated to be a tsetse fly salivary allergen, demonstrating that Antigen5-related proteins are represented as functional allergens not only in stinging but also in blood feeding insects.

KW - B780-tropical-medicine

KW - Entomology

KW - Tsetse flies

KW - Glossina morsitans morsitans

KW - Saliva

KW - Allergen

KW - TAg5

KW - Antigen 5

KW - Antibodies

KW - Immunoglobulin E

KW - Hypersensitivity

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:000267505200003

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DO - 10.1016/j.ibmb.2009.01.010

M3 - A1: Web of Science-article

SN - 0965-1748

VL - 39

SP - 332

EP - 341

JO - Insect Biochemistry and Molecular Biology

JF - Insect Biochemistry and Molecular Biology

IS - 5-6

ER -

Identification of a functional Antigen5-related allergen in the saliva of a blood feeding insect, the tsetse fly

Abstract

Keywords

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