Identification of molecular markers associated with alteration of receptor-binding specificity in a novel genotype of highly pathogenic avian influenza A(H5N1) viruses detected in Cambodia in 2013

Sareth Rith, C Todd Davis, Veasna Duong, Borann Sar, Srey Viseth Horm, Savuth Chin, Sovann Ly, Denis Laurent, Beat Richner, Ikwo Oboho, Yunho Jang, William Davis, Sharmi Thor, Amanda Balish, A Danielle Iuliano, San Sorn, Davun Holl, Touch Sok, Heng Seng, Arnaud TarantolaReiko Tsuyuoka, Amy Parry, Nora Chea, Lotfi Allal, Paul Kitsutani, Dora Warren, Michael Prouty, Paul Horwood, Marc-Alain Widdowson, Stephen Lindstrom, Julie Villanueva, Ruben Donis, Nancy Cox, Philippe Buchy

Research output: Contribution to journalA1: Web of Science-article

Abstract

Human infections with influenza A(H5N1) virus in Cambodia increased sharply during 2013. Molecular characterization of viruses detected in clinical specimens from human cases revealed the presence of mutations associated with the alteration of receptor-binding specificity (K189R, Q222L) and respiratory droplet transmission in ferrets (N220K with Q222L). Discovery of quasispecies at position 222 (Q/L), in addition to the absence of the mutations in poultry/environmental samples, suggested that the mutations occurred during human infection and did not transmit further.

Original languageEnglish
JournalJournal of Virology
Volume88
Issue number23
Pages (from-to)13897-13909
Number of pages13
ISSN0022-538X
DOIs
Publication statusPublished - 2014

Keywords

  • Adolescent
  • Adult
  • Amino Acid Substitution
  • Cambodia
  • Child
  • Child, Preschool
  • Cluster Analysis
  • Female
  • Genetic Markers
  • Genotype
  • Hemagglutinin Glycoproteins, Influenza Virus/genetics
  • Humans
  • Infant
  • Influenza A Virus, H5N1 Subtype/genetics
  • Influenza, Human/virology
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Mutation, Missense
  • Phylogeny
  • Sequence Analysis, DNA
  • Virus Attachment

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