Identification of molecular markers associated with alteration of receptor-binding specificity in a novel genotype of highly pathogenic avian influenza A(H5N1) viruses detected in Cambodia in 2013

Sareth Rith; C Todd Davis; Veasna Duong; Borann Sar; Srey Viseth Horm; Savuth Chin; Sovann Ly; Denis Laurent; Beat Richner; Ikwo Oboho; Yunho Jang; William Davis; Sharmi Thor; Amanda Balish; A Danielle Iuliano; San Sorn; Davun Holl; Touch Sok; Heng Seng; Arnaud Tarantola; Reiko Tsuyuoka; Amy Parry; Nora Chea; Lotfi Allal; Paul Kitsutani; Dora Warren; Michael Prouty; Paul Horwood; Marc-Alain Widdowson; Stephen Lindstrom; Julie Villanueva; Ruben Donis; Nancy Cox; Philippe Buchy

doi:10.1128/JVI.01887-14

Identification of molecular markers associated with alteration of receptor-binding specificity in a novel genotype of highly pathogenic avian influenza A(H5N1) viruses detected in Cambodia in 2013

Sareth Rith, C Todd Davis, Veasna Duong, Borann Sar, Srey Viseth Horm, Savuth Chin, Sovann Ly, Denis Laurent, Beat Richner, Ikwo Oboho, Yunho Jang, William Davis, Sharmi Thor, Amanda Balish, A Danielle Iuliano, San Sorn, Davun Holl, Touch Sok, Heng Seng, Arnaud TarantolaReiko Tsuyuoka, Amy Parry, Nora Chea, Lotfi Allal, Paul Kitsutani, Dora Warren, Michael Prouty, Paul Horwood, Marc-Alain Widdowson, Stephen Lindstrom, Julie Villanueva, Ruben Donis, Nancy Cox, Philippe Buchy

Institute of Tropical Medicine

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

Human infections with influenza A(H5N1) virus in Cambodia increased sharply during 2013. Molecular characterization of viruses detected in clinical specimens from human cases revealed the presence of mutations associated with the alteration of receptor-binding specificity (K189R, Q222L) and respiratory droplet transmission in ferrets (N220K with Q222L). Discovery of quasispecies at position 222 (Q/L), in addition to the absence of the mutations in poultry/environmental samples, suggested that the mutations occurred during human infection and did not transmit further.

Original language	English
Journal	Journal of Virology
Volume	88
Issue number	23
Pages (from-to)	13897-13909
Number of pages	13
ISSN	0022-538X
DOIs	https://doi.org/10.1128/JVI.01887-14
Publication status	Published - 2014

Keywords

Adolescent
Adult
Amino Acid Substitution
Cambodia
Child
Child, Preschool
Cluster Analysis
Female
Genetic Markers
Genotype
Hemagglutinin Glycoproteins, Influenza Virus/genetics
Humans
Infant
Influenza A Virus, H5N1 Subtype/genetics
Influenza, Human/virology
Male
Middle Aged
Molecular Sequence Data
Mutation, Missense
Phylogeny
Sequence Analysis, DNA
Virus Attachment

Access to Document

10.1128/JVI.01887-14

Cite this

Rith, S., Davis, C. T., Duong, V., Sar, B., Horm, S. V., Chin, S., Ly, S., Laurent, D., Richner, B., Oboho, I., Jang, Y., Davis, W., Thor, S., Balish, A., Iuliano, A. D., Sorn, S., Holl, D., Sok, T., Seng, H., ... Buchy, P. (2014). Identification of molecular markers associated with alteration of receptor-binding specificity in a novel genotype of highly pathogenic avian influenza A(H5N1) viruses detected in Cambodia in 2013. Journal of Virology, 88(23), 13897-13909. https://doi.org/10.1128/JVI.01887-14

@article{dc349198e81b43dd8912a8face845b5a,

title = "Identification of molecular markers associated with alteration of receptor-binding specificity in a novel genotype of highly pathogenic avian influenza A(H5N1) viruses detected in Cambodia in 2013",

abstract = "Human infections with influenza A(H5N1) virus in Cambodia increased sharply during 2013. Molecular characterization of viruses detected in clinical specimens from human cases revealed the presence of mutations associated with the alteration of receptor-binding specificity (K189R, Q222L) and respiratory droplet transmission in ferrets (N220K with Q222L). Discovery of quasispecies at position 222 (Q/L), in addition to the absence of the mutations in poultry/environmental samples, suggested that the mutations occurred during human infection and did not transmit further. ",

keywords = "Adolescent, Adult, Amino Acid Substitution, Cambodia, Child, Child, Preschool, Cluster Analysis, Female, Genetic Markers, Genotype, Hemagglutinin Glycoproteins, Influenza Virus/genetics, Humans, Infant, Influenza A Virus, H5N1 Subtype/genetics, Influenza, Human/virology, Male, Middle Aged, Molecular Sequence Data, Mutation, Missense, Phylogeny, Sequence Analysis, DNA, Virus Attachment",

author = "Sareth Rith and Davis, {C Todd} and Veasna Duong and Borann Sar and Horm, {Srey Viseth} and Savuth Chin and Sovann Ly and Denis Laurent and Beat Richner and Ikwo Oboho and Yunho Jang and William Davis and Sharmi Thor and Amanda Balish and Iuliano, {A Danielle} and San Sorn and Davun Holl and Touch Sok and Heng Seng and Arnaud Tarantola and Reiko Tsuyuoka and Amy Parry and Nora Chea and Lotfi Allal and Paul Kitsutani and Dora Warren and Michael Prouty and Paul Horwood and Marc-Alain Widdowson and Stephen Lindstrom and Julie Villanueva and Ruben Donis and Nancy Cox and Philippe Buchy",

year = "2014",

doi = "10.1128/JVI.01887-14",

language = "English",

volume = "88",

pages = "13897--13909",

journal = "Journal of Virology",

issn = "0022-538X",

publisher = "American Society for Microbiology",

number = "23",

}

Rith, S, Davis, CT, Duong, V, Sar, B, Horm, SV, Chin, S, Ly, S, Laurent, D, Richner, B, Oboho, I, Jang, Y, Davis, W, Thor, S, Balish, A, Iuliano, AD, Sorn, S, Holl, D, Sok, T, Seng, H, Tarantola, A, Tsuyuoka, R, Parry, A, Chea, N, Allal, L, Kitsutani, P, Warren, D, Prouty, M, Horwood, P, Widdowson, M-A, Lindstrom, S, Villanueva, J, Donis, R, Cox, N & Buchy, P 2014, 'Identification of molecular markers associated with alteration of receptor-binding specificity in a novel genotype of highly pathogenic avian influenza A(H5N1) viruses detected in Cambodia in 2013', Journal of Virology, vol. 88, no. 23, pp. 13897-13909. https://doi.org/10.1128/JVI.01887-14

Identification of molecular markers associated with alteration of receptor-binding specificity in a novel genotype of highly pathogenic avian influenza A(H5N1) viruses detected in Cambodia in 2013. / Rith, Sareth; Davis, C Todd; Duong, Veasna et al.
In: Journal of Virology, Vol. 88, No. 23, 2014, p. 13897-13909.

Research output: Contribution to journal › A1: Web of Science-article › peer-review

TY - JOUR

T1 - Identification of molecular markers associated with alteration of receptor-binding specificity in a novel genotype of highly pathogenic avian influenza A(H5N1) viruses detected in Cambodia in 2013

AU - Rith, Sareth

AU - Davis, C Todd

AU - Duong, Veasna

AU - Sar, Borann

AU - Horm, Srey Viseth

AU - Chin, Savuth

AU - Ly, Sovann

AU - Laurent, Denis

AU - Richner, Beat

AU - Oboho, Ikwo

AU - Jang, Yunho

AU - Davis, William

AU - Thor, Sharmi

AU - Balish, Amanda

AU - Iuliano, A Danielle

AU - Sorn, San

AU - Holl, Davun

AU - Sok, Touch

AU - Seng, Heng

AU - Tarantola, Arnaud

AU - Tsuyuoka, Reiko

AU - Parry, Amy

AU - Chea, Nora

AU - Allal, Lotfi

AU - Kitsutani, Paul

AU - Warren, Dora

AU - Prouty, Michael

AU - Horwood, Paul

AU - Widdowson, Marc-Alain

AU - Lindstrom, Stephen

AU - Villanueva, Julie

AU - Donis, Ruben

AU - Cox, Nancy

AU - Buchy, Philippe

PY - 2014

Y1 - 2014

N2 - Human infections with influenza A(H5N1) virus in Cambodia increased sharply during 2013. Molecular characterization of viruses detected in clinical specimens from human cases revealed the presence of mutations associated with the alteration of receptor-binding specificity (K189R, Q222L) and respiratory droplet transmission in ferrets (N220K with Q222L). Discovery of quasispecies at position 222 (Q/L), in addition to the absence of the mutations in poultry/environmental samples, suggested that the mutations occurred during human infection and did not transmit further.

AB - Human infections with influenza A(H5N1) virus in Cambodia increased sharply during 2013. Molecular characterization of viruses detected in clinical specimens from human cases revealed the presence of mutations associated with the alteration of receptor-binding specificity (K189R, Q222L) and respiratory droplet transmission in ferrets (N220K with Q222L). Discovery of quasispecies at position 222 (Q/L), in addition to the absence of the mutations in poultry/environmental samples, suggested that the mutations occurred during human infection and did not transmit further.

KW - Adolescent

KW - Adult

KW - Amino Acid Substitution

KW - Cambodia

KW - Child

KW - Child, Preschool

KW - Cluster Analysis

KW - Female

KW - Genetic Markers

KW - Genotype

KW - Hemagglutinin Glycoproteins, Influenza Virus/genetics

KW - Humans

KW - Infant

KW - Influenza A Virus, H5N1 Subtype/genetics

KW - Influenza, Human/virology

KW - Male

KW - Middle Aged

KW - Molecular Sequence Data

KW - Mutation, Missense

KW - Phylogeny

KW - Sequence Analysis, DNA

KW - Virus Attachment

U2 - 10.1128/JVI.01887-14

DO - 10.1128/JVI.01887-14

M3 - A1: Web of Science-article

C2 - 25210193

SN - 0022-538X

VL - 88

SP - 13897

EP - 13909

JO - Journal of Virology

JF - Journal of Virology

IS - 23

ER -

Identification of molecular markers associated with alteration of receptor-binding specificity in a novel genotype of highly pathogenic avian influenza A(H5N1) viruses detected in Cambodia in 2013

Abstract

Keywords

Access to Document

Fingerprint

Cite this