Identification of stage biomarkers for human African trypanosomiasis

DN Amin, DM Ngoyi, GM Nhkwachi, M Palomba, M Rottenberg, P Büscher, K Kristensson, W Masocha

    Research output: Contribution to journalA1: Web of Science-article

    Abstract

    Human African trypanosomiasis (HAT), caused by infection with sub-species of Trypanosoma brucei (T. b.), manifests as a hemolymphatic stage followed by an encephalitic stage. The distinction of the two stages needs improvement as drugs used for the late stage are highly toxic. Transcripts encoding 16 secreted proteins differentially expressed in the brains of mice at late stage T. b. brucei infection when the early stage drug suramin is no longer effective and different to immunoglobulins, chemokines, and cytokines, were selected by microarray analysis. Lipocalin 2 and secretory leukocyte peptidase inhibitor (SLPI) mRNA showed the highest differential expression in mice. These transcripts were also upregulated in brains from infected rats. Lipocalin 2 was increased in cerebrospinal fluid (CSF) from rats during late stage T. b. brucei infection. Protein levels of lipocalin 2, SLPI, and the chemokine CXCL10 were found increased in CSF from Trypanosoma brucei gambiense and Trypanosoma brucei rhodesiense late stage HAT compared to early stage
    Original languageEnglish
    JournalAmerican Journal of Tropical Medicine and Hygiene
    Volume82
    Issue number6
    Pages (from-to)983-990
    Number of pages8
    ISSN0002-9637
    DOIs
    Publication statusPublished - 2010

    Keywords

    • B780-tropical-medicine
    • Protozoal diseases
    • Trypanosomiasis-African
    • Sleeping sickness
    • Trypanosoma brucei brucei
    • Trypanosoma brucei gambiense
    • Trypanosoma brucei rhodesiense
    • Hemolymph
    • Encephalitic stage
    • Biomarkers
    • Identification
    • Treatment
    • Drug reactions
    • Toxicity
    • Protein expression
    • Leukocytes
    • Chemokines
    • Congo-Kinshasa
    • Africa-Central

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