Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis in Peru: assessment of a molecular step-wise approach

N Veland, AK Boggild, C Valencia, BM Valencia, A Llanos-Cuentas, G Van der Auwera, JC Dujardin, J Arevalo

Research output: Contribution to journalA1: Web of Science-article

Abstract

We present an algorithm based on three PCR assays for Leishmania (Viannia) species identification and assessed its performance using 70 specimens from Peruvian patients. The succession of the assayed targets can be ordered according to species prevalence. Sequential progression through the algorithm reduced the number of samples here studied by approximately 30% after each step.
Original languageEnglish
JournalJournal of Clinical Microbiology
Volume50
Issue number2
Pages (from-to)495-498
ISSN0095-1137
DOIs
Publication statusPublished - 2012

Keywords

  • B780-tropical-medicine
  • Protozoal diseases
  • Cutaneous
  • Leishmaniasis
  • Leishmania braziliensis
  • Leishmania guyanensis
  • Vectors
  • Sandflies
  • Lutzomyia
  • Identification
  • Isolation
  • Algorithms
  • Polymerase chain reaction
  • PCR
  • Amplification
  • Performance
  • Peru
  • America-Latin

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