Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis in Peru: assessment of a molecular step-wise approach

N Veland; AK Boggild; C Valencia; BM Valencia; A Llanos-Cuentas; G Van der Auwera; JC Dujardin; J Arevalo

doi:10.1128/JCM.05061-11

Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis in Peru: assessment of a molecular step-wise approach

N Veland
, AK Boggild
, C Valencia
, BM Valencia
, A Llanos-Cuentas
, G Van der Auwera
, JC Dujardin
, J Arevalo

Molecular Parasitology

Research output: Contribution to journal › A1: Peer-reviewed journal articles › peer-review

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Abstract

We present an algorithm based on three PCR assays for Leishmania (Viannia) species identification and assessed its performance using 70 specimens from Peruvian patients. The succession of the assayed targets can be ordered according to species prevalence. Sequential progression through the algorithm reduced the number of samples here studied by approximately 30% after each step.

Original language	English
Journal	Journal of Clinical Microbiology
Volume	50
Issue number	2
Pages (from-to)	495-498
ISSN	0095-1137
DOIs	https://doi.org/10.1128/JCM.05061-11
Publication status	Published - 2012

Keywords

B780-tropical-medicine
Protozoal diseases
Cutaneous
Leishmaniasis
Leishmania braziliensis
Leishmania guyanensis
Vectors
Sandflies
Lutzomyia
Identification
Isolation
Algorithms
Polymerase chain reaction
PCR
Amplification
Performance
Peru
America-Latin

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10.1128/JCM.05061-11

2012jcmi0495

http://lib.itg.be/pdf/itg/2012/2012jcmi0495.pdf

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@article{29095d6416214e5db85ccf314def0102,

title = "Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis in Peru: assessment of a molecular step-wise approach",

abstract = "We present an algorithm based on three PCR assays for Leishmania (Viannia) species identification and assessed its performance using 70 specimens from Peruvian patients. The succession of the assayed targets can be ordered according to species prevalence. Sequential progression through the algorithm reduced the number of samples here studied by approximately 30\% after each step.",

keywords = "B780-tropical-medicine, Protozoal diseases, Cutaneous, Leishmaniasis, Leishmania braziliensis, Leishmania guyanensis, Vectors, Sandflies, Lutzomyia, Identification, Isolation, Algorithms, Polymerase chain reaction, PCR, Amplification, Performance, Peru, America-Latin",

author = "N Veland and AK Boggild and C Valencia and BM Valencia and A Llanos-Cuentas and \{Van der Auwera\}, G and JC Dujardin and J Arevalo",

note = "FTX; ITG-B6A; ITG-B7A; DBM; U-MOLPAR; JIF; DOI; PDF; Abstract; DSPACE",

year = "2012",

doi = "10.1128/JCM.05061-11",

language = "English",

volume = "50",

pages = "495--498",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "2",

}

Veland, N, Boggild, AK, Valencia, C, Valencia, BM, Llanos-Cuentas, A, Van der Auwera, G , Dujardin, JC & Arevalo, J 2012, 'Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis in Peru: assessment of a molecular step-wise approach', Journal of Clinical Microbiology, vol. 50, no. 2, pp. 495-498. https://doi.org/10.1128/JCM.05061-11

TY - JOUR

T1 - Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis in Peru: assessment of a molecular step-wise approach

AU - Veland, N

AU - Boggild, AK

AU - Valencia, C

AU - Valencia, BM

AU - Llanos-Cuentas, A

AU - Van der Auwera, G

AU - Dujardin, JC

AU - Arevalo, J

N1 - FTX; ITG-B6A; ITG-B7A; DBM; U-MOLPAR; JIF; DOI; PDF; Abstract; DSPACE

PY - 2012

Y1 - 2012

N2 - We present an algorithm based on three PCR assays for Leishmania (Viannia) species identification and assessed its performance using 70 specimens from Peruvian patients. The succession of the assayed targets can be ordered according to species prevalence. Sequential progression through the algorithm reduced the number of samples here studied by approximately 30% after each step.

AB - We present an algorithm based on three PCR assays for Leishmania (Viannia) species identification and assessed its performance using 70 specimens from Peruvian patients. The succession of the assayed targets can be ordered according to species prevalence. Sequential progression through the algorithm reduced the number of samples here studied by approximately 30% after each step.

KW - B780-tropical-medicine

KW - Protozoal diseases

KW - Cutaneous

KW - Leishmaniasis

KW - Leishmania braziliensis

KW - Leishmania guyanensis

KW - Vectors

KW - Sandflies

KW - Lutzomyia

KW - Identification

KW - Isolation

KW - Algorithms

KW - Polymerase chain reaction

KW - PCR

KW - Amplification

KW - Performance

KW - Peru

KW - America-Latin

U2 - 10.1128/JCM.05061-11

DO - 10.1128/JCM.05061-11

M3 - A1: Peer-reviewed journal articles

C2 - 22116151

SN - 0095-1137

VL - 50

SP - 495

EP - 498

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 2

ER -

Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis in Peru: assessment of a molecular step-wise approach

Abstract

Keywords

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