Mycobacterium tuberculosis retains viability in RNAlater buffer but not in GTC-TCEP and DNA/RNA Shield

Research output: Contribution to journalA1: Web of Science-articlepeer-review

20 Downloads (Pure)

Abstract

Efficient inactivation of clinical samples containing mycobacteria is crucial for biosafety during shipment and handling. When stored in RNAlater, Mycobacterium tuberculosis H37Ra remains viable, and our results suggest that at -20 °C and 4 °C changes in the mycobacterial transcriptome are possible. Only GTC-TCEP and DNA/RNA Shield inactivate sufficiently for shipment.

Original languageEnglish
JournalDiagnostic Microbiology and Infectious Disease
Volume106
Issue number1
Pages (from-to)115905
Number of pages3
ISSN0732-8893
DOIs
Publication statusPublished - 2023

Keywords

  • DNA
  • Humans
  • Mycobacterium tuberculosis/genetics
  • RNA

Fingerprint

Dive into the research topics of 'Mycobacterium tuberculosis retains viability in RNAlater buffer but not in GTC-TCEP and DNA/RNA Shield'. Together they form a unique fingerprint.

Cite this