Integrated detection of multi- and extensively drug-resistant tuberculosis using the nitrate reductase assay

E Ramos; K Fissette; P. De Rijk; JC Palomino; A. Martin

doi:10.5588/ijtld.11.0129

Integrated detection of multi- and extensively drug-resistant tuberculosis using the nitrate reductase assay

E Ramos, K Fissette, P. De Rijk, JC Palomino, A. Martin

Mycobacteriology

Research output: Contribution to journal › A1: Web of Science-article › peer-review

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Abstract

It currently takes 2-3 months to obtain a diagnosis for multidrug-resistant (MDR-) and extensively drug-resistant tuberculosis (XDR-TB). We evaluated the rapid non-commercial nitrate reductase assay (NRA), which is capable of the simultaneous detection of MDR- and XDR-TB, and compared the results with the proportion method (PM). The sensitivity was respectively 97%, 99%, 100% and 94.6% for rifampicin (RMP), isoniazid (INH), ofloxacin (OFX) and kanamycin (KM). The specificity was respectively 100%, 95%, 95.7% and 99% for RMP, INH, OFX and KM. The turnaround time for NRA was 10-14 days, compared to 4-6 weeks for the PM. Our study showed that NRA provided sensitive and specific detection of resistance to first- and second-line drugs.

Original language	English
Journal	International Journal of Tuberculosis and Lung Disease
Volume	16
Issue number	1
Pages (from-to)	110-113
ISSN	1027-3719
DOIs	https://doi.org/10.5588/ijtld.11.0129
Publication status	Published - 2012

Keywords

B780-tropical-medicine
Bacterial diseases
Tuberculosis
Mycobacterium tuberculosis
Multidrug resistance
Integrated
Detection
Nitrate reductase assay
Sensitivity
Specificity
Rifampicin
Isoniazid
Ofloxacin
Kanamycin
Second-line drugs
First-line drugs
Laboratory techniques and procedures

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10.5588/ijtld.11.0129

2012ijtl0110

http://lib.itg.be/pdf/itg/2012/2012ijtl0110.pdf

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title = "Integrated detection of multi- and extensively drug-resistant tuberculosis using the nitrate reductase assay",

abstract = "It currently takes 2-3 months to obtain a diagnosis for multidrug-resistant (MDR-) and extensively drug-resistant tuberculosis (XDR-TB). We evaluated the rapid non-commercial nitrate reductase assay (NRA), which is capable of the simultaneous detection of MDR- and XDR-TB, and compared the results with the proportion method (PM). The sensitivity was respectively 97%, 99%, 100% and 94.6% for rifampicin (RMP), isoniazid (INH), ofloxacin (OFX) and kanamycin (KM). The specificity was respectively 100%, 95%, 95.7% and 99% for RMP, INH, OFX and KM. The turnaround time for NRA was 10-14 days, compared to 4-6 weeks for the PM. Our study showed that NRA provided sensitive and specific detection of resistance to first- and second-line drugs.",

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author = "E Ramos and K Fissette and {De Rijk}, P. and JC Palomino and A. Martin",

note = "FTX; ITG-B1B; ITG-B2B; ITG-B3B; ITG-B4A; ITG-BLA; DBM; U-MYCOB; JIF; DOI; PDF; Abstract; DSPACE; CHAU",

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doi = "10.5588/ijtld.11.0129",

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AU - Ramos, E

AU - Fissette, K

AU - De Rijk, P.

AU - Palomino, JC

AU - Martin, A.

N1 - FTX; ITG-B1B; ITG-B2B; ITG-B3B; ITG-B4A; ITG-BLA; DBM; U-MYCOB; JIF; DOI; PDF; Abstract; DSPACE; CHAU

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Y1 - 2012

N2 - It currently takes 2-3 months to obtain a diagnosis for multidrug-resistant (MDR-) and extensively drug-resistant tuberculosis (XDR-TB). We evaluated the rapid non-commercial nitrate reductase assay (NRA), which is capable of the simultaneous detection of MDR- and XDR-TB, and compared the results with the proportion method (PM). The sensitivity was respectively 97%, 99%, 100% and 94.6% for rifampicin (RMP), isoniazid (INH), ofloxacin (OFX) and kanamycin (KM). The specificity was respectively 100%, 95%, 95.7% and 99% for RMP, INH, OFX and KM. The turnaround time for NRA was 10-14 days, compared to 4-6 weeks for the PM. Our study showed that NRA provided sensitive and specific detection of resistance to first- and second-line drugs.

AB - It currently takes 2-3 months to obtain a diagnosis for multidrug-resistant (MDR-) and extensively drug-resistant tuberculosis (XDR-TB). We evaluated the rapid non-commercial nitrate reductase assay (NRA), which is capable of the simultaneous detection of MDR- and XDR-TB, and compared the results with the proportion method (PM). The sensitivity was respectively 97%, 99%, 100% and 94.6% for rifampicin (RMP), isoniazid (INH), ofloxacin (OFX) and kanamycin (KM). The specificity was respectively 100%, 95%, 95.7% and 99% for RMP, INH, OFX and KM. The turnaround time for NRA was 10-14 days, compared to 4-6 weeks for the PM. Our study showed that NRA provided sensitive and specific detection of resistance to first- and second-line drugs.

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KW - Bacterial diseases

KW - Tuberculosis

KW - Mycobacterium tuberculosis

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KW - Integrated

KW - Detection

KW - Nitrate reductase assay

KW - Sensitivity

KW - Specificity

KW - Rifampicin

KW - Isoniazid

KW - Ofloxacin

KW - Kanamycin

KW - Second-line drugs

KW - First-line drugs

KW - Laboratory techniques and procedures

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DO - 10.5588/ijtld.11.0129

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SN - 1027-3719

VL - 16

SP - 110

EP - 113

JO - International Journal of Tuberculosis and Lung Disease

JF - International Journal of Tuberculosis and Lung Disease

IS - 1

ER -

Integrated detection of multi- and extensively drug-resistant tuberculosis using the nitrate reductase assay

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Keywords

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