Integrated detection of multi- and extensively drug-resistant tuberculosis using the nitrate reductase assay

E Ramos, K Fissette, P. De Rijk, JC Palomino, A. Martin

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Abstract

It currently takes 2-3 months to obtain a diagnosis for multidrug-resistant (MDR-) and extensively drug-resistant tuberculosis (XDR-TB). We evaluated the rapid non-commercial nitrate reductase assay (NRA), which is capable of the simultaneous detection of MDR- and XDR-TB, and compared the results with the proportion method (PM). The sensitivity was respectively 97%, 99%, 100% and 94.6% for rifampicin (RMP), isoniazid (INH), ofloxacin (OFX) and kanamycin (KM). The specificity was respectively 100%, 95%, 95.7% and 99% for RMP, INH, OFX and KM. The turnaround time for NRA was 10-14 days, compared to 4-6 weeks for the PM. Our study showed that NRA provided sensitive and specific detection of resistance to first- and second-line drugs.
Original languageEnglish
JournalInternational Journal of Tuberculosis and Lung Disease
Volume16
Issue number1
Pages (from-to)110-113
ISSN1027-3719
DOIs
Publication statusPublished - 2012

Keywords

  • B780-tropical-medicine
  • Bacterial diseases
  • Tuberculosis
  • Mycobacterium tuberculosis
  • Multidrug resistance
  • Integrated
  • Detection
  • Nitrate reductase assay
  • Sensitivity
  • Specificity
  • Rifampicin
  • Isoniazid
  • Ofloxacin
  • Kanamycin
  • Second-line drugs
  • First-line drugs
  • Laboratory techniques and procedures

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