Interpatient genetic variability of HIV-1 group O

W Janssens; L Heyndrickx; G Van der Auwera; J Nkengasong; E Beirnaert; K Vereecken; S Coppens; B Willems; K Fransen; M Peeters; P Ndumbe; E Delaporte; G van der Groen

doi:10.1097/00002030-199901140-00006

Interpatient genetic variability of HIV-1 group O

W Janssens, L Heyndrickx, G Van der Auwera, J Nkengasong, E Beirnaert, K Vereecken, S Coppens, B Willems, K Fransen, M Peeters, P Ndumbe, E Delaporte, G van der Groen

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

Objective:

To analyse the genetic and phylogenetic characteristics of HIV-1 group O viruses.

Materials and methods:

The env gene, encoding the gp160 glycoprotein, and a partial p24-encoding gag gene fragment of a Cameroonian (CA9) and a Gabonese (VI686) HIV-1 group O virus, isolated from cultured peripheral blood mononuclear cells of symptomatic patients, were sequenced, aligned with other representatives of group O for which the same region has been documented, and genetically and phylogenetically analysed.

Results:

Phylogenetic analysis of the env gene (gp160) revealed that CA9, VI686, ANT70, and four Ha strains formed a separate cluster, which was supported by 100% of all bootstrap trees. In addition, these seven isolates were part of the same clade in the p24 phylogeny. VAU and MVP5180 may represent two other subtypes.

Conclusion:

We have characterized two group O viruses, originating from Cameroon and Gabon, which show a close evolutionary relationship to ANT70 and four Ha strains based on the entire env gene, suggestive of a first group O subgroup, tentatively named the HIV-1 group O env ANT70 clade or subtype.

Original language	English
Journal	AIDS
Volume	13
Issue number	1
Pages (from-to)	41-48
ISSN	0269-9370
DOIs	https://doi.org/10.1097/00002030-199901140-00006
Publication status	Published - 1999

Keywords

B780-tropical-medicine
Virology
HIV-1 group O
Subtypes
Phylogenetics
Cameroon
Gabon
Africa-Central

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10.1097/00002030-199901140-00006

http://lib.itg.be/pdf/itg/1999/1999aids0041.pdf

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title = "Interpatient genetic variability of HIV-1 group O",

abstract = "Objective: To analyse the genetic and phylogenetic characteristics of HIV-1 group O viruses.Materials and methods: The env gene, encoding the gp160 glycoprotein, and a partial p24-encoding gag gene fragment of a Cameroonian (CA9) and a Gabonese (VI686) HIV-1 group O virus, isolated from cultured peripheral blood mononuclear cells of symptomatic patients, were sequenced, aligned with other representatives of group O for which the same region has been documented, and genetically and phylogenetically analysed.Results: Phylogenetic analysis of the env gene (gp160) revealed that CA9, VI686, ANT70, and four Ha strains formed a separate cluster, which was supported by 100% of all bootstrap trees. In addition, these seven isolates were part of the same clade in the p24 phylogeny. VAU and MVP5180 may represent two other subtypes.Conclusion: We have characterized two group O viruses, originating from Cameroon and Gabon, which show a close evolutionary relationship to ANT70 and four Ha strains based on the entire env gene, suggestive of a first group O subgroup, tentatively named the HIV-1 group O env ANT70 clade or subtype.",

keywords = "B780-tropical-medicine, Virology, HIV-1 group O, Subtypes, Phylogenetics, Cameroon, Gabon, Africa-Central",

author = "W Janssens and L Heyndrickx and {Van der Auwera}, G and J Nkengasong and E Beirnaert and K Vereecken and S Coppens and B Willems and K Fransen and M Peeters and P Ndumbe and E Delaporte and {van der Groen}, G",

year = "1999",

doi = "10.1097/00002030-199901140-00006",

language = "English",

volume = "13",

pages = "41--48",

journal = "AIDS",

issn = "0269-9370",

publisher = "Lippincott Williams & Wilkins",

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TY - JOUR

T1 - Interpatient genetic variability of HIV-1 group O

AU - Janssens, W

AU - Heyndrickx, L

AU - Van der Auwera, G

AU - Nkengasong, J

AU - Beirnaert, E

AU - Vereecken, K

AU - Coppens, S

AU - Willems, B

AU - Fransen, K

AU - Peeters, M

AU - Ndumbe, P

AU - Delaporte, E

AU - van der Groen, G

PY - 1999

Y1 - 1999

N2 - Objective: To analyse the genetic and phylogenetic characteristics of HIV-1 group O viruses.Materials and methods: The env gene, encoding the gp160 glycoprotein, and a partial p24-encoding gag gene fragment of a Cameroonian (CA9) and a Gabonese (VI686) HIV-1 group O virus, isolated from cultured peripheral blood mononuclear cells of symptomatic patients, were sequenced, aligned with other representatives of group O for which the same region has been documented, and genetically and phylogenetically analysed.Results: Phylogenetic analysis of the env gene (gp160) revealed that CA9, VI686, ANT70, and four Ha strains formed a separate cluster, which was supported by 100% of all bootstrap trees. In addition, these seven isolates were part of the same clade in the p24 phylogeny. VAU and MVP5180 may represent two other subtypes.Conclusion: We have characterized two group O viruses, originating from Cameroon and Gabon, which show a close evolutionary relationship to ANT70 and four Ha strains based on the entire env gene, suggestive of a first group O subgroup, tentatively named the HIV-1 group O env ANT70 clade or subtype.

AB - Objective: To analyse the genetic and phylogenetic characteristics of HIV-1 group O viruses.Materials and methods: The env gene, encoding the gp160 glycoprotein, and a partial p24-encoding gag gene fragment of a Cameroonian (CA9) and a Gabonese (VI686) HIV-1 group O virus, isolated from cultured peripheral blood mononuclear cells of symptomatic patients, were sequenced, aligned with other representatives of group O for which the same region has been documented, and genetically and phylogenetically analysed.Results: Phylogenetic analysis of the env gene (gp160) revealed that CA9, VI686, ANT70, and four Ha strains formed a separate cluster, which was supported by 100% of all bootstrap trees. In addition, these seven isolates were part of the same clade in the p24 phylogeny. VAU and MVP5180 may represent two other subtypes.Conclusion: We have characterized two group O viruses, originating from Cameroon and Gabon, which show a close evolutionary relationship to ANT70 and four Ha strains based on the entire env gene, suggestive of a first group O subgroup, tentatively named the HIV-1 group O env ANT70 clade or subtype.

KW - B780-tropical-medicine

KW - Virology

KW - HIV-1 group O

KW - Subtypes

KW - Phylogenetics

KW - Cameroon

KW - Gabon

KW - Africa-Central

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:000079246400006

U2 - 10.1097/00002030-199901140-00006

DO - 10.1097/00002030-199901140-00006

M3 - A1: Web of Science-article

SN - 0269-9370

VL - 13

SP - 41

EP - 48

JO - AIDS

JF - AIDS

IS - 1

ER -

Interpatient genetic variability of HIV-1 group O

Abstract

Keywords

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