Intrapulmonary percussive ventilation improves lung function in cystic fibrosis patients chronically colonized with Pseudomonas aeruginosa: a pilot cross-over study

Jozef Dingemans, Hanneke Eyns, Julie Willekens, Pieter Monsieurs, Rob Van Houdt, Pierre Cornelis, Anne Malfroot, Aurélie Crabbé

Research output: Contribution to journalA1: Web of Science-article

Abstract

High levels of shear stress can prevent and disrupt Pseudomonas aeruginosa biofilm formation in vitro. Intrapulmonary percussive ventilation (IPV) could be used to introduce shear stress into the lungs of cystic fibrosis (CF) patients to disrupt biofilms in vivo. We performed a first-of-its-kind pilot clinical study to evaluate short-term IPV therapy at medium (200 bursts per minute, bpm) and high frequency (400 bpm) as compared to autogenic drainage (AD) on lung function and the behavior of P. aeruginosa in the CF lung in four patients who are chronically colonized by P. aeruginosa. A significant difference between the three treatment groups was observed for both the forced expiratory volume in 1 s (FEV1) and the forced vital capacity (FVC) (p < 0.05). More specifically, IPV at high frequency significantly increased FEV1 and FVC compared to AD (p < 0.05) and IPV at medium frequency (p < 0.001). IPV at high frequency enhanced the expression levels of P. aeruginosa planktonic marker genes, which was less pronounced with IPV at medium frequency or AD. In conclusion, IPV at high frequency could potentially alter the behavior of P. aeruginosa in the CF lung and improve lung function.

TRIAL REGISTRATION: The trail was retrospectively registered at the ISRCTN registry on 6 June 2013, under trial registration number ISRCTN75391385.

Original languageEnglish
JournalEuropean Journal of Clinical Microbiology and Infectious Diseases
Volume37
Issue number6
Pages (from-to)1143-1151
Number of pages9
ISSN0934-9723
DOIs
Publication statusPublished - 2018
Externally publishedYes

Keywords

  • Adult
  • Biofilms/growth & development
  • Cross-Over Studies
  • Cystic Fibrosis/genetics
  • Female
  • Humans
  • Lung/microbiology
  • Male
  • Mutation
  • Percussion/instrumentation
  • Pilot Projects
  • Pseudomonas Infections/microbiology
  • Pseudomonas aeruginosa/isolation & purification
  • Respiratory Function Tests/methods
  • Retrospective Studies
  • Sputum/microbiology
  • Ventilation/methods
  • Young Adult

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