Trypanosoma brucei gambiense: HMI-9 medium containing methylcellulose and human serum supports the continuous axenic in vitro propagation of the bloodstream form

N van Reet, PP Pyana, S Deborggraeve, P Büscher, F Claes

    Research output: Contribution to journalA1: Web of Science-article

    Abstract

    Trypanosoma brucei (T.b.) gambiense causes the chronic form of human African trypanosomiasis or sleeping sickness. One of the major problems with studying T.b. gambiense is the difficulty to isolate it from its original host and the difficult adaptation to in vivo and in vitro mass propagation. The objective of this study was to evaluate if an established method for axenic culture of pleomorphic bloodstream form T.b. brucei strains, based on methylcellulose containing HMI-9 medium, also facilitated the continuous in vitro propagation of other bloodstream form Trypanozoon strains, in particular of T.b. gambiense. Bloodstream form trypanosomes from one T.b. brucei, two T.b. rhodesiense, one T. evansi and seven T.b. gambiense strains were isolated from mouse blood and each was concurrently cultivated in liquid and methylcellulose-containing HMI-9 based medium, either with or without additional human serum supplementation, for over 10 consecutive sub passages. Although HMI-9 based medium supplemented with 1.1% (w/v) methylcellulose supported the continuous cultivation of all non-gambiense strains better than liquid media could, the in vitro cultivation of all gambiense strains was only achieved in HMI-9 based medium containing 1.1% (w/v) methylcellulose, 15% (v/v) fetal calf serum and 5% (v/v) heat-inactivated human serum.
    Original languageEnglish
    JournalExperimental Parasitology
    Volume128
    Issue number3
    Pages (from-to)285-290
    Number of pages6
    ISSN0014-4894
    DOIs
    Publication statusPublished - 2011

    Keywords

    • B780-tropical-medicine
    • Protozoal diseases
    • Trypanosomiasis-African
    • Sleeping sickness
    • Trypanosoma brucei gambiense
    • Trypanosoma brucei rhodesiense
    • Evaluation
    • Methods
    • In vitro
    • In vivo
    • Isolation
    • Adaptation
    • Blood
    • Strains
    • Cultivation
    • Methylcellulose
    • HMI-9
    • Serum
    • Liquid
    • Media
    • Laboratory techniques and procedures

    Fingerprint Dive into the research topics of '<i>Trypanosoma brucei gambiense</i>: HMI-9 medium containing methylcellulose and human serum supports the continuous axenic in vitro propagation of the bloodstream form'. Together they form a unique fingerprint.

    Cite this