Knowledge of morphology is still required when identifying new amoeba isolates by molecular techniques

J.F. De Jonckheere, S. Gryseels, M. Eddyani

Research output: Contribution to journalA1: Web of Science-article

Abstract

We have isolated several free-living amoeba strains from the environment in Ghana, which have internal transcribed spacers, including the 5.8S rDNA, sequences similar to sequences attributed to Vahlkampfiidae (Heterolobosea) in databases. However, morphological examination shows that the isolates belong to the Hartmannellidae (Amoebozoa). We provide evidence that the sequences in the databases are wrongly classified as belonging to a genus or species of the Vahlkampfiidae, but rather belong to strains of the genus Hartmannella.
Original languageEnglish
JournalEuropean Journal of Protistology
Volume48
Issue number3
Pages (from-to)178-184
Number of pages7
ISSN0932-4739
DOIs
Publication statusPublished - 2012

Keywords

  • Amoeba
  • Hartmannella
  • Vahlkampfia
  • Morphology
  • Identification
  • Molecular diagnostic techniques
  • rDNA
  • Classification
  • Laboratory techniques and procedures
  • Ghana
  • Africa-West

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