Knowledge of morphology is still required when identifying new amoeba isolates by molecular techniques

J.F. De Jonckheere; S. Gryseels; M. Eddyani

doi:10.1016/j.ejop.2012.01.009

Knowledge of morphology is still required when identifying new amoeba isolates by molecular techniques

J.F. De Jonckheere, S. Gryseels, M. Eddyani

Mycobacteriology

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

We have isolated several free-living amoeba strains from the environment in Ghana, which have internal transcribed spacers, including the 5.8S rDNA, sequences similar to sequences attributed to Vahlkampfiidae (Heterolobosea) in databases. However, morphological examination shows that the isolates belong to the Hartmannellidae (Amoebozoa). We provide evidence that the sequences in the databases are wrongly classified as belonging to a genus or species of the Vahlkampfiidae, but rather belong to strains of the genus Hartmannella.

Original language	English
Journal	European Journal of Protistology
Volume	48
Issue number	3
Pages (from-to)	178-184
Number of pages	7
ISSN	0932-4739
DOIs	https://doi.org/10.1016/j.ejop.2012.01.009
Publication status	Published - 2012

Keywords

Amoeba
Hartmannella
Vahlkampfia
Morphology
Identification
Molecular diagnostic techniques
rDNA
Classification
Laboratory techniques and procedures
Ghana
Africa-West

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10.1016/j.ejop.2012.01.009

http://www.ncbi.nlm.nih.gov/pubmed/22425548

Cite this

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title = "Knowledge of morphology is still required when identifying new amoeba isolates by molecular techniques",

abstract = "We have isolated several free-living amoeba strains from the environment in Ghana, which have internal transcribed spacers, including the 5.8S rDNA, sequences similar to sequences attributed to Vahlkampfiidae (Heterolobosea) in databases. However, morphological examination shows that the isolates belong to the Hartmannellidae (Amoebozoa). We provide evidence that the sequences in the databases are wrongly classified as belonging to a genus or species of the Vahlkampfiidae, but rather belong to strains of the genus Hartmannella.",

keywords = "Amoeba, Hartmannella, Vahlkampfia, Morphology, Identification, Molecular diagnostic techniques, rDNA, Classification, Laboratory techniques and procedures, Ghana, Africa-West",

author = "{De Jonckheere}, J.F. and S. Gryseels and M. Eddyani",

note = "NPP; ITG-BLB; DBM; U-MYCOB; JIF; DOI; Not available; Abstract; DSPACE",

year = "2012",

doi = "10.1016/j.ejop.2012.01.009",

language = "English",

volume = "48",

pages = "178--184",

journal = "European Journal of Protistology",

issn = "0932-4739",

publisher = "Urban und Fischer Verlag GmbH und Co. KG",

number = "3",

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TY - JOUR

T1 - Knowledge of morphology is still required when identifying new amoeba isolates by molecular techniques

AU - De Jonckheere, J.F.

AU - Gryseels, S.

AU - Eddyani, M.

N1 - NPP; ITG-BLB; DBM; U-MYCOB; JIF; DOI; Not available; Abstract; DSPACE

PY - 2012

Y1 - 2012

N2 - We have isolated several free-living amoeba strains from the environment in Ghana, which have internal transcribed spacers, including the 5.8S rDNA, sequences similar to sequences attributed to Vahlkampfiidae (Heterolobosea) in databases. However, morphological examination shows that the isolates belong to the Hartmannellidae (Amoebozoa). We provide evidence that the sequences in the databases are wrongly classified as belonging to a genus or species of the Vahlkampfiidae, but rather belong to strains of the genus Hartmannella.

AB - We have isolated several free-living amoeba strains from the environment in Ghana, which have internal transcribed spacers, including the 5.8S rDNA, sequences similar to sequences attributed to Vahlkampfiidae (Heterolobosea) in databases. However, morphological examination shows that the isolates belong to the Hartmannellidae (Amoebozoa). We provide evidence that the sequences in the databases are wrongly classified as belonging to a genus or species of the Vahlkampfiidae, but rather belong to strains of the genus Hartmannella.

KW - Amoeba

KW - Hartmannella

KW - Vahlkampfia

KW - Morphology

KW - Identification

KW - Molecular diagnostic techniques

KW - rDNA

KW - Classification

KW - Laboratory techniques and procedures

KW - Ghana

KW - Africa-West

U2 - 10.1016/j.ejop.2012.01.009

DO - 10.1016/j.ejop.2012.01.009

M3 - A1: Web of Science-article

C2 - 22425548

SN - 0932-4739

VL - 48

SP - 178

EP - 184

JO - European Journal of Protistology

JF - European Journal of Protistology

IS - 3

ER -

Knowledge of morphology is still required when identifying new amoeba isolates by molecular techniques

Abstract

Keywords

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