Low dose irradiation of thyroid cells reveals a unique transcriptomic and epigenetic signature in RET/PTC-positive cells

Khalil Abou-El-Ardat; Pieter Monsieurs; Natasa Anastasov; Mike Atkinson; Hanane Derradji; Tim De Meyer; Sofie Bekaert; Wim Van Criekinge; Sarah Baatout

doi:10.1016/j.mrfmmm.2011.10.006

Low dose irradiation of thyroid cells reveals a unique transcriptomic and epigenetic signature in RET/PTC-positive cells

Khalil Abou-El-Ardat, Pieter Monsieurs, Natasa Anastasov, Mike Atkinson, Hanane Derradji, Tim De Meyer, Sofie Bekaert, Wim Van Criekinge, Sarah Baatout

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

The high doses of radiation received in the wake of the Chernobyl incident and the atomic bombing of Hiroshima and Nagasaki have been linked to the increased appearance of thyroid cancer in the children living in the vicinity of the site. However, the data gathered on the effect of low doses of radiation on the thyroid remain limited. We have examined the genome wide transcriptional response of a culture of TPC-1 human cell line of papillary thyroid carcinoma origin with a RET/PTC1 translocation to various doses (0.0625, 0.5, and 4 Gy) of X-rays and compared it to response of thyroids with a RET/PTC3 translocation and against wild-type mouse thyroids irradiated with the same doses using Affymetrix microarrays. We have found considerable overlap at a high dose of 4 Gy in both RET/PTC-positive systems but no common genes at 62.5 mGy. In addition, the response of RET/PTC-positive system at all doses was distinct from the response of wild-type thyroids with both systems signaling down different pathways. Analysis of the response of microRNAs in TPC-1 cells revealed a radiation-responsive signature of microRNAs in addition to dose-responsive microRNAs. Our results point to the fact that a low dose of X-rays seems to have a significant proliferative effect on normal thyroids. This observation should be studied further as opposed to its effect on RET/PTC-positive thyroids which was subtle, anti-proliferative and system-dependent. (C) 2011 Elsevier By. All rights reserved.

Original language	English
Journal	Mutation Research
Volume	731
Issue number	1-2
Pages (from-to)	27-40
Number of pages	14
ISSN	0027-5107
DOIs	https://doi.org/10.1016/j.mrfmmm.2011.10.006
Publication status	Published - 1-Mar-2012
Externally published	Yes

Keywords

RET/PTC translocation
Thyroid
Micro RNA
X-irradiation, Low doses
MACROPHAGE INHIBITORY CYTOKINE-1
PERIPHERAL-BLOOD LYMPHOCYTES
RECEPTOR TYROSINE KINASE
IONIZING-RADIATION
GENE-EXPRESSION
TUMOR-SUPPRESSOR
PAPILLARY CARCINOMAS
HUMAN FIBROBLASTS
TRANSGENIC MICE
POST-CHERNOBYL

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10.1016/j.mrfmmm.2011.10.006

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@article{01543c85486e41a89b26f09ce93815c1,

title = "Low dose irradiation of thyroid cells reveals a unique transcriptomic and epigenetic signature in RET/PTC-positive cells",

abstract = "The high doses of radiation received in the wake of the Chernobyl incident and the atomic bombing of Hiroshima and Nagasaki have been linked to the increased appearance of thyroid cancer in the children living in the vicinity of the site. However, the data gathered on the effect of low doses of radiation on the thyroid remain limited. We have examined the genome wide transcriptional response of a culture of TPC-1 human cell line of papillary thyroid carcinoma origin with a RET/PTC1 translocation to various doses (0.0625, 0.5, and 4 Gy) of X-rays and compared it to response of thyroids with a RET/PTC3 translocation and against wild-type mouse thyroids irradiated with the same doses using Affymetrix microarrays. We have found considerable overlap at a high dose of 4 Gy in both RET/PTC-positive systems but no common genes at 62.5 mGy. In addition, the response of RET/PTC-positive system at all doses was distinct from the response of wild-type thyroids with both systems signaling down different pathways. Analysis of the response of microRNAs in TPC-1 cells revealed a radiation-responsive signature of microRNAs in addition to dose-responsive microRNAs. Our results point to the fact that a low dose of X-rays seems to have a significant proliferative effect on normal thyroids. This observation should be studied further as opposed to its effect on RET/PTC-positive thyroids which was subtle, anti-proliferative and system-dependent. (C) 2011 Elsevier By. All rights reserved.",

keywords = "RET/PTC translocation, Thyroid, Micro RNA, X-irradiation, Low doses, MACROPHAGE INHIBITORY CYTOKINE-1, PERIPHERAL-BLOOD LYMPHOCYTES, RECEPTOR TYROSINE KINASE, IONIZING-RADIATION, GENE-EXPRESSION, TUMOR-SUPPRESSOR, PAPILLARY CARCINOMAS, HUMAN FIBROBLASTS, TRANSGENIC MICE, POST-CHERNOBYL",

author = "Khalil Abou-El-Ardat and Pieter Monsieurs and Natasa Anastasov and Mike Atkinson and Hanane Derradji and {De Meyer}, Tim and Sofie Bekaert and {Van Criekinge}, Wim and Sarah Baatout",

year = "2012",

month = mar,

day = "1",

doi = "10.1016/j.mrfmmm.2011.10.006",

language = "English",

volume = "731",

pages = "27--40",

journal = "Mutation Research",

issn = "0027-5107",

publisher = "Elsevier",

number = "1-2",

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TY - JOUR

T1 - Low dose irradiation of thyroid cells reveals a unique transcriptomic and epigenetic signature in RET/PTC-positive cells

AU - Abou-El-Ardat, Khalil

AU - Monsieurs, Pieter

AU - Anastasov, Natasa

AU - Atkinson, Mike

AU - Derradji, Hanane

AU - De Meyer, Tim

AU - Bekaert, Sofie

AU - Van Criekinge, Wim

AU - Baatout, Sarah

PY - 2012/3/1

Y1 - 2012/3/1

N2 - The high doses of radiation received in the wake of the Chernobyl incident and the atomic bombing of Hiroshima and Nagasaki have been linked to the increased appearance of thyroid cancer in the children living in the vicinity of the site. However, the data gathered on the effect of low doses of radiation on the thyroid remain limited. We have examined the genome wide transcriptional response of a culture of TPC-1 human cell line of papillary thyroid carcinoma origin with a RET/PTC1 translocation to various doses (0.0625, 0.5, and 4 Gy) of X-rays and compared it to response of thyroids with a RET/PTC3 translocation and against wild-type mouse thyroids irradiated with the same doses using Affymetrix microarrays. We have found considerable overlap at a high dose of 4 Gy in both RET/PTC-positive systems but no common genes at 62.5 mGy. In addition, the response of RET/PTC-positive system at all doses was distinct from the response of wild-type thyroids with both systems signaling down different pathways. Analysis of the response of microRNAs in TPC-1 cells revealed a radiation-responsive signature of microRNAs in addition to dose-responsive microRNAs. Our results point to the fact that a low dose of X-rays seems to have a significant proliferative effect on normal thyroids. This observation should be studied further as opposed to its effect on RET/PTC-positive thyroids which was subtle, anti-proliferative and system-dependent. (C) 2011 Elsevier By. All rights reserved.

AB - The high doses of radiation received in the wake of the Chernobyl incident and the atomic bombing of Hiroshima and Nagasaki have been linked to the increased appearance of thyroid cancer in the children living in the vicinity of the site. However, the data gathered on the effect of low doses of radiation on the thyroid remain limited. We have examined the genome wide transcriptional response of a culture of TPC-1 human cell line of papillary thyroid carcinoma origin with a RET/PTC1 translocation to various doses (0.0625, 0.5, and 4 Gy) of X-rays and compared it to response of thyroids with a RET/PTC3 translocation and against wild-type mouse thyroids irradiated with the same doses using Affymetrix microarrays. We have found considerable overlap at a high dose of 4 Gy in both RET/PTC-positive systems but no common genes at 62.5 mGy. In addition, the response of RET/PTC-positive system at all doses was distinct from the response of wild-type thyroids with both systems signaling down different pathways. Analysis of the response of microRNAs in TPC-1 cells revealed a radiation-responsive signature of microRNAs in addition to dose-responsive microRNAs. Our results point to the fact that a low dose of X-rays seems to have a significant proliferative effect on normal thyroids. This observation should be studied further as opposed to its effect on RET/PTC-positive thyroids which was subtle, anti-proliferative and system-dependent. (C) 2011 Elsevier By. All rights reserved.

KW - RET/PTC translocation

KW - Thyroid

KW - Micro RNA

KW - X-irradiation, Low doses

KW - MACROPHAGE INHIBITORY CYTOKINE-1

KW - PERIPHERAL-BLOOD LYMPHOCYTES

KW - RECEPTOR TYROSINE KINASE

KW - IONIZING-RADIATION

KW - GENE-EXPRESSION

KW - TUMOR-SUPPRESSOR

KW - PAPILLARY CARCINOMAS

KW - HUMAN FIBROBLASTS

KW - TRANSGENIC MICE

KW - POST-CHERNOBYL

U2 - 10.1016/j.mrfmmm.2011.10.006

DO - 10.1016/j.mrfmmm.2011.10.006

M3 - A1: Web of Science-article

SN - 0027-5107

VL - 731

SP - 27

EP - 40

JO - Mutation Research

JF - Mutation Research

IS - 1-2

ER -

Low dose irradiation of thyroid cells reveals a unique transcriptomic and epigenetic signature in RET/PTC-positive cells

Abstract

Keywords

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