M48U1 CD4 mimetic has a sustained inhibitory effect on cell-associated HIV-1 by attenuating virion infectivity through gp120 shedding

P. Selhorst; K. Grupping; T. Tong; E.T. Crooks; L. Martin; G. Vanham; J.M. Binley; K.K. Ariën

doi:10.1186/1742-4690-10-12

M48U1 CD4 mimetic has a sustained inhibitory effect on cell-associated HIV-1 by attenuating virion infectivity through gp120 shedding

P. Selhorst, K. Grupping, T. Tong, E.T. Crooks, L. Martin, G. Vanham, J.M. Binley, K.K. Ariën

Virology

Research output: Contribution to journal › A1: Web of Science-article › peer-review

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Abstract

BACKGROUND: HIV-1 infected cells can establish new infections by crossing the vaginal epithelia and subsequently producing virus in a milieu that avoids the high microbicide concentrations of the vaginal lumen. FINDINGS: To address this problem, here, we report that pretreatment of HIV-infected peripheral blood mononuclear cells (PBMCs) with a 27 amino acid CD4-mimetic, M48U1, causes dramatic and prolonged reduction of infectious virus output, due to its induction of gp120 shedding. CONCLUSIONS: M48U1 may, therefore, be valuable for prophylaxis of mucosal HIV-1 transmission.

Original language	English
Journal	Retrovirology
Volume	10
Issue number	12
Pages (from-to)	1-9
Number of pages	9
ISSN	1742-4690
DOIs	https://doi.org/10.1186/1742-4690-10-12
Publication status	Published - 2013

Keywords

Viral diseases
HIV-1
AIDS
Infectivity
Epithelial cells
Vagina
Peripheral blood mononuclear cell (PBMC)
Amino acids
CD4
GP120
Prophylaxis
Inhibition
Development

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10.1186/1742-4690-10-12

2013retr0012Final published version, 1.18 MB

CHAARM: Combined Highly Active Anti-Retroviral Microbicides
Vanham, G., Buvé, A. & Janssens, N.
European Commission
1/01/10 → 30/06/15
Project: Research Project

Cite this

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title = "M48U1 CD4 mimetic has a sustained inhibitory effect on cell-associated HIV-1 by attenuating virion infectivity through gp120 shedding",

abstract = "BACKGROUND: HIV-1 infected cells can establish new infections by crossing the vaginal epithelia and subsequently producing virus in a milieu that avoids the high microbicide concentrations of the vaginal lumen. FINDINGS: To address this problem, here, we report that pretreatment of HIV-infected peripheral blood mononuclear cells (PBMCs) with a 27 amino acid CD4-mimetic, M48U1, causes dramatic and prolonged reduction of infectious virus output, due to its induction of gp120 shedding. CONCLUSIONS: M48U1 may, therefore, be valuable for prophylaxis of mucosal HIV-1 transmission.",

keywords = "Viral diseases, HIV-1, AIDS, Infectivity, Epithelial cells, Vagina, Peripheral blood mononuclear cell (PBMC), Amino acids, CD4, GP120, Prophylaxis, Inhibition, Development",

author = "P. Selhorst and K. Grupping and T. Tong and E.T. Crooks and L. Martin and G. Vanham and J.M. Binley and K.K. Ari{\"e}n",

note = "ITG-B1B; ITG-B2B; ITG-B6A; ITG-BLA; DBM; U-VIROL; JIF; DOI; PDF; URL; E-only; Abstract; DSPACE",

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doi = "10.1186/1742-4690-10-12",

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TY - JOUR

T1 - M48U1 CD4 mimetic has a sustained inhibitory effect on cell-associated HIV-1 by attenuating virion infectivity through gp120 shedding

AU - Selhorst, P.

AU - Grupping, K.

AU - Tong, T.

AU - Crooks, E.T.

AU - Martin, L.

AU - Vanham, G.

AU - Binley, J.M.

AU - Ariën, K.K.

N1 - ITG-B1B; ITG-B2B; ITG-B6A; ITG-BLA; DBM; U-VIROL; JIF; DOI; PDF; URL; E-only; Abstract; DSPACE

PY - 2013

Y1 - 2013

N2 - BACKGROUND: HIV-1 infected cells can establish new infections by crossing the vaginal epithelia and subsequently producing virus in a milieu that avoids the high microbicide concentrations of the vaginal lumen. FINDINGS: To address this problem, here, we report that pretreatment of HIV-infected peripheral blood mononuclear cells (PBMCs) with a 27 amino acid CD4-mimetic, M48U1, causes dramatic and prolonged reduction of infectious virus output, due to its induction of gp120 shedding. CONCLUSIONS: M48U1 may, therefore, be valuable for prophylaxis of mucosal HIV-1 transmission.

AB - BACKGROUND: HIV-1 infected cells can establish new infections by crossing the vaginal epithelia and subsequently producing virus in a milieu that avoids the high microbicide concentrations of the vaginal lumen. FINDINGS: To address this problem, here, we report that pretreatment of HIV-infected peripheral blood mononuclear cells (PBMCs) with a 27 amino acid CD4-mimetic, M48U1, causes dramatic and prolonged reduction of infectious virus output, due to its induction of gp120 shedding. CONCLUSIONS: M48U1 may, therefore, be valuable for prophylaxis of mucosal HIV-1 transmission.

KW - Viral diseases

KW - HIV-1

KW - AIDS

KW - Infectivity

KW - Epithelial cells

KW - Vagina

KW - Peripheral blood mononuclear cell (PBMC)

KW - Amino acids

KW - CD4

KW - GP120

KW - Prophylaxis

KW - Inhibition

KW - Development

U2 - 10.1186/1742-4690-10-12

DO - 10.1186/1742-4690-10-12

M3 - A1: Web of Science-article

C2 - 23375046

SN - 1742-4690

VL - 10

SP - 1

EP - 9

JO - Retrovirology

JF - Retrovirology

IS - 12

ER -

M48U1 CD4 mimetic has a sustained inhibitory effect on cell-associated HIV-1 by attenuating virion infectivity through gp120 shedding

Abstract

Keywords

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Projects

CHAARM: Combined Highly Active Anti-Retroviral Microbicides

Cite this