Measurement of recent exposure to Phlebotomus argentipes, the vector of Indian visceral leishmaniasis, by using human antibody responses to sand fly saliva

MF Clements, K Gidwani, R Kumar, J Hostomska, DS Dinesh, V Kumar, P Das, I Muller, G Hamilton, V Volfova, M Boelaert, M Das, S Rijal, A Picado, P Volf, S Sundar, CR Davies, ME Rogers

    Research output: Contribution to journalA1: Web of Science-article

    Abstract

    Antibody (IgG) responses to the saliva of Phlebotomus argentipes were investigated using serum samples from regions of India endemic and non-endemic for visceral leishmaniasis (VL). By pre-adsorbing the sera against the saliva of the competing human-biting but non-VL vector P. papatasi, we significantly improved the specificity of a P. argentipes saliva enzyme-linked immunosorbent assay. Using this method, we observed a statistically significant correlation between antibodies to P. argenitpes saliva and the average indoor density of female sand flies. Additionally, the method was able to detect recent changes in vector exposure when sera from VL patients were assayed before, during, and after hospitalization and protected from sand fly bites under untreated bed nets. Collectively, these results highlight the utility of antibodies to P. argentipes saliva as an important tool to evaluate VL vector control programs
    Original languageEnglish
    JournalAmerican Journal of Tropical Medicine and Hygiene
    Volume82
    Issue number5
    Pages (from-to)801-807
    Number of pages7
    ISSN0002-9637
    DOIs
    Publication statusPublished - 2010

    Keywords

    • B780-tropical-medicine
    • Protozoal diseases
    • Leishmaniasis
    • Visceral
    • Kala azar
    • Leishmania donovani
    • Vectors
    • Sandflies
    • Phlebotomus argentipes
    • Saliva
    • Antibodies
    • Immunoglobulin G
    • Enzyme-linked immunosorbent assay (ELISA)
    • ELISA
    • Serum
    • Vector density
    • Exposure
    • India
    • Asia-South

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