Molecular characterization of antigen 24, a specific immunodominant antigen family from Leishmania infantum

B Couvreur; D Jacquet; A Bollen; D Le Ray

doi:10.1017/S0031182097001844

Molecular characterization of antigen 24, a specific immunodominant antigen family from Leishmania infantum

B Couvreur, D Jacquet, A Bollen, D Le Ray

Institute of Tropical Medicine

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

Leishmania infantum immunoelectrophoretic antigen 24 (AG 24), a visceral leishmaniasis associated immunodominant antigen, has been characterized with a monospecific antiserum by combining SDS–PAGE, immunoblotting, metabolic labelling, radio-immunoprecipitation and in vitro poly A⁺ mRNA translation. AG 24 appeared to correspond to a multi-antigen family of 6–9 members ranging from 20 to 31 kDa and proteinic by nature with no post-translational modifications. A similar banding pattern was recognized by infection sera. AG 24 was not found exposed on the cell surface.

Original language	English
Journal	Parasitology
Volume	115
Issue number	6
Pages (from-to)	611-619
Number of pages	9
ISSN	0031-1820
DOIs	https://doi.org/10.1017/S0031182097001844
Publication status	Published - 1997

Keywords

B780-tropical-medicine
Protozoology
Molecular biology
Leishmania infantum
Serology
Electrophoresis
Immunoelectrophoresis
Laboratory medicine
Diagnosis

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10.1017/S0031182097001844

http://lib.itg.be/pdf/itg/1997/1997para0611.pdf

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title = "Molecular characterization of antigen 24, a specific immunodominant antigen family from Leishmania infantum",

abstract = "Leishmania infantum immunoelectrophoretic antigen 24 (AG 24), a visceral leishmaniasis associated immunodominant antigen, has been characterized with a monospecific antiserum by combining SDS–PAGE, immunoblotting, metabolic labelling, radio-immunoprecipitation and in vitro poly A+ mRNA translation. AG 24 appeared to correspond to a multi-antigen family of 6–9 members ranging from 20 to 31 kDa and proteinic by nature with no post-translational modifications. A similar banding pattern was recognized by infection sera. AG 24 was not found exposed on the cell surface.",

keywords = "B780-tropical-medicine, Protozoology, Molecular biology, Leishmania infantum, Serology, Electrophoresis, Immunoelectrophoresis, Laboratory medicine, Diagnosis",

author = "B Couvreur and D Jacquet and A Bollen and {Le Ray}, D",

note = "FTX: Abonnement",

year = "1997",

doi = "10.1017/S0031182097001844",

language = "English",

volume = "115",

pages = "611--619",

journal = "Parasitology",

issn = "0031-1820",

publisher = "Cambridge University Press",

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TY - JOUR

T1 - Molecular characterization of antigen 24, a specific immunodominant antigen family from Leishmania infantum

AU - Couvreur, B

AU - Jacquet, D

AU - Bollen, A

AU - Le Ray, D

N1 - FTX: Abonnement

PY - 1997

Y1 - 1997

N2 - Leishmania infantum immunoelectrophoretic antigen 24 (AG 24), a visceral leishmaniasis associated immunodominant antigen, has been characterized with a monospecific antiserum by combining SDS–PAGE, immunoblotting, metabolic labelling, radio-immunoprecipitation and in vitro poly A+ mRNA translation. AG 24 appeared to correspond to a multi-antigen family of 6–9 members ranging from 20 to 31 kDa and proteinic by nature with no post-translational modifications. A similar banding pattern was recognized by infection sera. AG 24 was not found exposed on the cell surface.

AB - Leishmania infantum immunoelectrophoretic antigen 24 (AG 24), a visceral leishmaniasis associated immunodominant antigen, has been characterized with a monospecific antiserum by combining SDS–PAGE, immunoblotting, metabolic labelling, radio-immunoprecipitation and in vitro poly A+ mRNA translation. AG 24 appeared to correspond to a multi-antigen family of 6–9 members ranging from 20 to 31 kDa and proteinic by nature with no post-translational modifications. A similar banding pattern was recognized by infection sera. AG 24 was not found exposed on the cell surface.

KW - B780-tropical-medicine

KW - Protozoology

KW - Molecular biology

KW - Leishmania infantum

KW - Serology

KW - Electrophoresis

KW - Immunoelectrophoresis

KW - Laboratory medicine

KW - Diagnosis

UR - https://www.webofscience.com/wos/woscc/full-record/WOS:000071657500006

U2 - 10.1017/S0031182097001844

DO - 10.1017/S0031182097001844

M3 - A1: Web of Science-article

SN - 0031-1820

VL - 115

SP - 611

EP - 619

JO - Parasitology

JF - Parasitology

IS - 6

ER -

Molecular characterization of antigen 24, a specific immunodominant antigen family from Leishmania infantum

Abstract

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