Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrhea: a cross-sectional study in four provinces of Mozambique diarrheagenic Escherichia coli in Mozambique

Lena Manhique-Coutinho, Paola Chiani, Valeria Michelacci, Elisa Taviani, Adilson Fernando Loforte Bauhofer, Assucenio Chissaque, Idalecia Cossa-Moiane, Julia Sambo, Jorfelia Chilaule, Esperanca Lourenco Guimaraes, Judite Salencia, Marta Cassocera, Diocreciano Matias Bero, Jose Paulo Langa, Nilsa de Deus

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    Abstract

    Objectives: Analyze the frequency of diarrheagenic Escherichia coli (DEC) pathotypes and their antimicrobial resistance profiles among children aged

    Methods: A cross-sectional hospital-based surveillance program of diarrhea was implemented in Maputo, Sofala, Zambezia, and Nampula. A single stool sample was collected from each child from May 2014 to May 2017. Culture methods and biochemical characterization were performed to detect E. coli strains. DEC pathotypes were determined by conventional polymerase chain reaction targeting specific virulence genes. Antimicrobial susceptibility was assessed by the Kirby-Bauer method.

    Results: From 723 specimens analyzed by culture, 262 were positive for E. coli. A total of 208 samples were tested by polymerase chain reaction for DEC identification, of which 101 (48.6%) were positive for a DEC pathotype. The predominant pathotypes were enteroaggregative (66.3%, 67/101), enteropathogenic (15.8%, 16/101), enterotoxigenic (13.9%, 14/101), and enteroinvasive E. coli (4.0%, 4/101). No Shiga toxin-producing E. coli was identified. Regardless of the province, the most frequent pathotype was enteroaggregative E. coli. Isolated DEC presented high frequency of resistance to ampicillin (97.8%), tetracycline (68.3%), chloramphenicol (28.4%), nalidixic acid (19.5%), and gentamicin (14.4%).

    Conclusion: Children with diarrhea in Mozambique had DEC and higher resistance to ampicillin and tetracycline. (C) 2022 Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.

    Original languageEnglish
    JournalInternational Journal of Infectious Diseases
    Volume121
    Pages (from-to)190-194
    Number of pages5
    ISSN1201-9712
    DOIs
    Publication statusPublished - 2022

    Keywords

    • Escherichia coli
    • Diarrheagenic E. coli pathotypes
    • Antibiotics resistance
    • Children
    • Mozambique
    • MAPUTO
    • TOXIN
    • AGE

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