TY - JOUR
T1 - Molecular characterization of diarrheagenic Escherichia coli isolates from children with diarrhea: a cross-sectional study in four provinces of Mozambique diarrheagenic Escherichia coli in Mozambique
AU - Manhique-Coutinho, Lena
AU - Chiani, Paola
AU - Michelacci, Valeria
AU - Taviani, Elisa
AU - Loforte Bauhofer, Adilson Fernando
AU - Chissaque, Assucenio
AU - Cossa-Moiane, Idalecia
AU - Sambo, Julia
AU - Chilaule, Jorfelia
AU - Guimaraes, Esperanca Lourenco
AU - Salencia, Judite
AU - Cassocera, Marta
AU - Bero, Diocreciano Matias
AU - Langa, Jose Paulo
AU - de Deus, Nilsa
N1 - FTX; DOAJ; (CC BY-NC-ND 4.0)
PY - 2022
Y1 - 2022
N2 - Objectives: Analyze the frequency of diarrheagenic Escherichia coli (DEC) pathotypes and their antimicrobial resistance profiles among children agedMethods: A cross-sectional hospital-based surveillance program of diarrhea was implemented in Maputo, Sofala, Zambezia, and Nampula. A single stool sample was collected from each child from May 2014 to May 2017. Culture methods and biochemical characterization were performed to detect E. coli strains. DEC pathotypes were determined by conventional polymerase chain reaction targeting specific virulence genes. Antimicrobial susceptibility was assessed by the Kirby-Bauer method.Results: From 723 specimens analyzed by culture, 262 were positive for E. coli. A total of 208 samples were tested by polymerase chain reaction for DEC identification, of which 101 (48.6%) were positive for a DEC pathotype. The predominant pathotypes were enteroaggregative (66.3%, 67/101), enteropathogenic (15.8%, 16/101), enterotoxigenic (13.9%, 14/101), and enteroinvasive E. coli (4.0%, 4/101). No Shiga toxin-producing E. coli was identified. Regardless of the province, the most frequent pathotype was enteroaggregative E. coli. Isolated DEC presented high frequency of resistance to ampicillin (97.8%), tetracycline (68.3%), chloramphenicol (28.4%), nalidixic acid (19.5%), and gentamicin (14.4%).Conclusion: Children with diarrhea in Mozambique had DEC and higher resistance to ampicillin and tetracycline. (C) 2022 Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.
AB - Objectives: Analyze the frequency of diarrheagenic Escherichia coli (DEC) pathotypes and their antimicrobial resistance profiles among children agedMethods: A cross-sectional hospital-based surveillance program of diarrhea was implemented in Maputo, Sofala, Zambezia, and Nampula. A single stool sample was collected from each child from May 2014 to May 2017. Culture methods and biochemical characterization were performed to detect E. coli strains. DEC pathotypes were determined by conventional polymerase chain reaction targeting specific virulence genes. Antimicrobial susceptibility was assessed by the Kirby-Bauer method.Results: From 723 specimens analyzed by culture, 262 were positive for E. coli. A total of 208 samples were tested by polymerase chain reaction for DEC identification, of which 101 (48.6%) were positive for a DEC pathotype. The predominant pathotypes were enteroaggregative (66.3%, 67/101), enteropathogenic (15.8%, 16/101), enterotoxigenic (13.9%, 14/101), and enteroinvasive E. coli (4.0%, 4/101). No Shiga toxin-producing E. coli was identified. Regardless of the province, the most frequent pathotype was enteroaggregative E. coli. Isolated DEC presented high frequency of resistance to ampicillin (97.8%), tetracycline (68.3%), chloramphenicol (28.4%), nalidixic acid (19.5%), and gentamicin (14.4%).Conclusion: Children with diarrhea in Mozambique had DEC and higher resistance to ampicillin and tetracycline. (C) 2022 Published by Elsevier Ltd on behalf of International Society for Infectious Diseases.
KW - Escherichia coli
KW - Diarrheagenic E. coli pathotypes
KW - Antibiotics resistance
KW - Children
KW - Mozambique
KW - MAPUTO
KW - TOXIN
KW - AGE
U2 - 10.1016/j.ijid.2022.04.054
DO - 10.1016/j.ijid.2022.04.054
M3 - A1: Web of Science-article
SN - 1201-9712
VL - 121
SP - 190
EP - 194
JO - International Journal of Infectious Diseases
JF - International Journal of Infectious Diseases
ER -