Molecular differentiation of Entamoeba spp. in a rural community of Loja province, South Ecuador

B Levecke; L Dreesen; M Barrionuevo-Samaniego; W Benitez Ortiz; N Praet; J Brandt; P Dorny

doi:10.1016/j.trstmh.2011.08.010

Molecular differentiation of Entamoeba spp. in a rural community of Loja province, South Ecuador

B Levecke, L Dreesen, M Barrionuevo-Samaniego, W Benitez Ortiz, N Praet, J Brandt, P Dorny

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

Although previous epidemiological surveys in Ecuador indicate the presence of Entamoeba histolytica, prevalence data of this parasite remain scarce. Most of the studies were based on microscopic examination, which does not allow a morphological differentiation from the non-pathogenic Ent. dispar and Ent. moshkovskii. In the present study, 674 stool samples from a South Ecuadorian rural community were screened for Entamoeba spp. Subsequently, molecular identification was performed on 101 samples containing Ent. histolytica/Ent. dispar/Ent. moshkovskii cysts. The study indicated the absence of Ent. histolytica in this South Ecuadorian community and confirmed the difficulty of differentiating Entamoeba spp. based on morphological features.

Original language	English
Journal	Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume	105
Issue number	12
Pages (from-to)	737-739
Number of pages	3
ISSN	0035-9203
DOIs	https://doi.org/10.1016/j.trstmh.2011.08.010
Publication status	Published - 2011

Keywords

B780-tropical-medicine
Protozoal diseases
Amebiasis
Entamoeba histolytica
Entamoeba dispar
Entamoeba moshkovskii
Molecular epidemiology
Prevalence
Morphology
Differentiation
Screening
PCR
Rural
Ecuador
America-Latin

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@article{f7d2214dfafc4dcd92e9023afb1ae846,

title = "Molecular differentiation of Entamoeba spp. in a rural community of Loja province, South Ecuador",

abstract = "Although previous epidemiological surveys in Ecuador indicate the presence of Entamoeba histolytica, prevalence data of this parasite remain scarce. Most of the studies were based on microscopic examination, which does not allow a morphological differentiation from the non-pathogenic Ent. dispar and Ent. moshkovskii. In the present study, 674 stool samples from a South Ecuadorian rural community were screened for Entamoeba spp. Subsequently, molecular identification was performed on 101 samples containing Ent. histolytica/Ent. dispar/Ent. moshkovskii cysts. The study indicated the absence of Ent. histolytica in this South Ecuadorian community and confirmed the difficulty of differentiating Entamoeba spp. based on morphological features.",

keywords = "B780-tropical-medicine, Protozoal diseases, Amebiasis, Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, Molecular epidemiology, Prevalence, Morphology, Differentiation, Screening, PCR, Rural, Ecuador, America-Latin",

author = "B Levecke and L Dreesen and M Barrionuevo-Samaniego and {Benitez Ortiz}, W and N Praet and J Brandt and P Dorny",

note = "NPP; ITG-A5A; ITG-X6A; ITG-ALA; ANIMAL; U-VHELM; JIF; DOI; PDF; Abstract; DSPACE",

year = "2011",

doi = "10.1016/j.trstmh.2011.08.010",

language = "English",

volume = "105",

pages = "737--739",

journal = "Transactions of the Royal Society of Tropical Medicine and Hygiene",

issn = "0035-9203",

publisher = "Oxford University Press",

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TY - JOUR

T1 - Molecular differentiation of Entamoeba spp. in a rural community of Loja province, South Ecuador

AU - Levecke, B

AU - Dreesen, L

AU - Barrionuevo-Samaniego, M

AU - Benitez Ortiz, W

AU - Praet, N

AU - Brandt, J

AU - Dorny, P

N1 - NPP; ITG-A5A; ITG-X6A; ITG-ALA; ANIMAL; U-VHELM; JIF; DOI; PDF; Abstract; DSPACE

PY - 2011

Y1 - 2011

N2 - Although previous epidemiological surveys in Ecuador indicate the presence of Entamoeba histolytica, prevalence data of this parasite remain scarce. Most of the studies were based on microscopic examination, which does not allow a morphological differentiation from the non-pathogenic Ent. dispar and Ent. moshkovskii. In the present study, 674 stool samples from a South Ecuadorian rural community were screened for Entamoeba spp. Subsequently, molecular identification was performed on 101 samples containing Ent. histolytica/Ent. dispar/Ent. moshkovskii cysts. The study indicated the absence of Ent. histolytica in this South Ecuadorian community and confirmed the difficulty of differentiating Entamoeba spp. based on morphological features.

AB - Although previous epidemiological surveys in Ecuador indicate the presence of Entamoeba histolytica, prevalence data of this parasite remain scarce. Most of the studies were based on microscopic examination, which does not allow a morphological differentiation from the non-pathogenic Ent. dispar and Ent. moshkovskii. In the present study, 674 stool samples from a South Ecuadorian rural community were screened for Entamoeba spp. Subsequently, molecular identification was performed on 101 samples containing Ent. histolytica/Ent. dispar/Ent. moshkovskii cysts. The study indicated the absence of Ent. histolytica in this South Ecuadorian community and confirmed the difficulty of differentiating Entamoeba spp. based on morphological features.

KW - B780-tropical-medicine

KW - Protozoal diseases

KW - Amebiasis

KW - Entamoeba histolytica

KW - Entamoeba dispar

KW - Entamoeba moshkovskii

KW - Molecular epidemiology

KW - Prevalence

KW - Morphology

KW - Differentiation

KW - Screening

KW - PCR

KW - Rural

KW - Ecuador

KW - America-Latin

U2 - 10.1016/j.trstmh.2011.08.010

DO - 10.1016/j.trstmh.2011.08.010

M3 - A1: Web of Science-article

C2 - 21981992

SN - 0035-9203

VL - 105

SP - 737

EP - 739

JO - Transactions of the Royal Society of Tropical Medicine and Hygiene

JF - Transactions of the Royal Society of Tropical Medicine and Hygiene

IS - 12

ER -

Molecular differentiation of Entamoeba spp. in a rural community of Loja province, South Ecuador

Abstract

Keywords

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