Molecular differentiation of Entamoeba spp. in a rural community of Loja province, South Ecuador

B Levecke, L Dreesen, M Barrionuevo-Samaniego, W Benitez Ortiz, N Praet, J Brandt, P Dorny

    Research output: Contribution to journalA1: Web of Science-article

    Abstract

    Although previous epidemiological surveys in Ecuador indicate the presence of Entamoeba histolytica, prevalence data of this parasite remain scarce. Most of the studies were based on microscopic examination, which does not allow a morphological differentiation from the non-pathogenic Ent. dispar and Ent. moshkovskii. In the present study, 674 stool samples from a South Ecuadorian rural community were screened for Entamoeba spp. Subsequently, molecular identification was performed on 101 samples containing Ent. histolytica/Ent. dispar/Ent. moshkovskii cysts. The study indicated the absence of Ent. histolytica in this South Ecuadorian community and confirmed the difficulty of differentiating Entamoeba spp. based on morphological features.
    Original languageEnglish
    JournalTransactions of the Royal Society of Tropical Medicine and Hygiene
    Volume105
    Issue number12
    Pages (from-to)737-739
    Number of pages3
    ISSN0035-9203
    DOIs
    Publication statusPublished - 2011

    Keywords

    • B780-tropical-medicine
    • Protozoal diseases
    • Amebiasis
    • Entamoeba histolytica
    • Entamoeba dispar
    • Entamoeba moshkovskii
    • Molecular epidemiology
    • Prevalence
    • Morphology
    • Differentiation
    • Screening
    • PCR
    • Rural
    • Ecuador
    • America-Latin

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