TY - JOUR
T1 - Molecular surveillance of malaria using the PF AmpliSeq custom assay for Plasmodium falciparum parasites from dried blood spot DNA isolates from Peru
AU - Kattenberg, Johanna Helena
AU - Van Dijk, Norbert J
AU - Fernández-Miñope, Carlos A
AU - Guetens, Pieter
AU - Mutsaers, Mathijs
AU - Gamboa, Dionicia
AU - Rosanas-Urgell, Anna
N1 - FTX; CINTEXT2; Copyright © 2023 The Authors; This is an open access article under the CC BY license (https://creativecommons.org/licenses/by/4.0/). (CC BY-NC 4.0)
PY - 2023
Y1 - 2023
N2 - Malaria molecular surveillance has great potential to support national malaria control programs (NMCPs), informing policy for its control and elimination. Here, we present a new three-day workflow for targeted resequencing of markers in 13 resistance-associated genes, histidine rich protein 2 and 3 (hrp2&3), a country (Peru)-specific 28 SNP-barcode for population genetic analysis, and apical membrane antigen 1 (ama1), using Illumina short-read sequencing technology. The assay applies a multiplex PCR approach to amplify all genomic regions of interest in a rapid and easily standardizable procedure and allows simultaneous amplification of a high number of targets at once, therefore having great potential for implementation into routine surveillance practice by NMCPs. The assay can be performed on routinely collected filter paper blood spots and can be easily adapted to different regions to investigate either regional trends or in-country epidemiological changes.
AB - Malaria molecular surveillance has great potential to support national malaria control programs (NMCPs), informing policy for its control and elimination. Here, we present a new three-day workflow for targeted resequencing of markers in 13 resistance-associated genes, histidine rich protein 2 and 3 (hrp2&3), a country (Peru)-specific 28 SNP-barcode for population genetic analysis, and apical membrane antigen 1 (ama1), using Illumina short-read sequencing technology. The assay applies a multiplex PCR approach to amplify all genomic regions of interest in a rapid and easily standardizable procedure and allows simultaneous amplification of a high number of targets at once, therefore having great potential for implementation into routine surveillance practice by NMCPs. The assay can be performed on routinely collected filter paper blood spots and can be easily adapted to different regions to investigate either regional trends or in-country epidemiological changes.
U2 - 10.21769/BioProtoc.4621
DO - 10.21769/BioProtoc.4621
M3 - A1: Web of Science-article
C2 - 36908639
SN - 2331-8325
VL - 13
SP - e4621
JO - Bio-Protocol
JF - Bio-Protocol
IS - 5
ER -