TY - JOUR
T1 - Molecular typing of Mycobacterium bovis from cattle reared in Midwest Brazil
AU - Tavares Carvalho, Ricardo Cesar
AU - Goncalves Vasconcellos, Sidra Ezidio
AU - Issa, Marina de Azevedo
AU - Soares Filho, Paulo Martins
AU - Pinto Coelho Mota, Pedro Moacyr
AU - de Araujo, Flabio Ribeiro
AU - da Silva Carvalho, Ana Carolina
AU - Gomes, Harrison Magdinier
AU - Suffys, Philip Noel
AU - de Souza Figueiredo, Eduardo Eustaquio
AU - Flosi Paschoalin, Vania Margaret
N1 - FTX; DOAJ
PY - 2016
Y1 - 2016
N2 - Mycobacterium bovis is the causative agent of bovine tuberculosis (BTB), the pathogen responsible for serious economic impact on the livestock sector. In order to obtain data on isolated M. bovis strains and assist in the control and eradication program for BTB, a cross sectional descriptive molecular epidemiology study in the Brazilian Midwest was conducted. Through spoligotyping and 24-loci MIRU-VNTR methods, 37 clinical isolates of M. bovis circulating in the region were analyzed, 10 isolated from the state of Mato Grosso, 12 from the state of Mato Grosso do Sul and 15 from the state of Goias. The spoligotyping analysis identified 10 distinct M. bovis profiles (SB0121 n = 14, SB0295 n = 6, SB0140 n = 6, SB0881 n = 3, SB1144 n = 2, SB1145 n = 2, SB0134 n = 1, SB1050 n = 1, SB1055 n = 1, SB1136 n = 1) grouped in six clusters and four orphan patterns. The MIRU-VNTR 24-loci grouped the same isolates in six clusters and 22 unique orphan patterns, showing higher discriminatory power than spoligotyping. When associating the results of both techniques, the isolates were grouped in five clusters and 24 unique M. bovis profiles. Among the 24-loci MIRUVNTR evaluated, two, ETR-A and QUB 11b loci, showed high discriminatory ability (h = >= 0.50), while MIRU 16, MIRU 27, ETR-B, ETR-C, Mtub21 and QUB 26 loci showed moderate ability (h = 0.33 or h = 0.49) and were the most effective in evaluating the genotypic similarities among the clinical M. bovis isolate samples. Herein, the 29 patterns found amongst the 37 isolates of M. bovis circulating in the Brazilian Midwest can be due to the animal movement between regions, municipalities and farms, thus causing the spread of various M. bovis strains in herds from Midwest Brazil.
AB - Mycobacterium bovis is the causative agent of bovine tuberculosis (BTB), the pathogen responsible for serious economic impact on the livestock sector. In order to obtain data on isolated M. bovis strains and assist in the control and eradication program for BTB, a cross sectional descriptive molecular epidemiology study in the Brazilian Midwest was conducted. Through spoligotyping and 24-loci MIRU-VNTR methods, 37 clinical isolates of M. bovis circulating in the region were analyzed, 10 isolated from the state of Mato Grosso, 12 from the state of Mato Grosso do Sul and 15 from the state of Goias. The spoligotyping analysis identified 10 distinct M. bovis profiles (SB0121 n = 14, SB0295 n = 6, SB0140 n = 6, SB0881 n = 3, SB1144 n = 2, SB1145 n = 2, SB0134 n = 1, SB1050 n = 1, SB1055 n = 1, SB1136 n = 1) grouped in six clusters and four orphan patterns. The MIRU-VNTR 24-loci grouped the same isolates in six clusters and 22 unique orphan patterns, showing higher discriminatory power than spoligotyping. When associating the results of both techniques, the isolates were grouped in five clusters and 24 unique M. bovis profiles. Among the 24-loci MIRUVNTR evaluated, two, ETR-A and QUB 11b loci, showed high discriminatory ability (h = >= 0.50), while MIRU 16, MIRU 27, ETR-B, ETR-C, Mtub21 and QUB 26 loci showed moderate ability (h = 0.33 or h = 0.49) and were the most effective in evaluating the genotypic similarities among the clinical M. bovis isolate samples. Herein, the 29 patterns found amongst the 37 isolates of M. bovis circulating in the Brazilian Midwest can be due to the animal movement between regions, municipalities and farms, thus causing the spread of various M. bovis strains in herds from Midwest Brazil.
KW - TUBERCULOSIS COMPLEX BACTERIA
KW - LENGTH-POLYMORPHISM ANALYSIS
KW - MATO-GROSSO
KW - DISCRIMINATORY ABILITY
KW - SPOLIGOTYPE DIVERSITY
KW - GENETIC DIVERSITY
KW - REPETITIVE UNITS
KW - HIGH-RESOLUTION
KW - TANDEM REPEATS
KW - STRAINS
U2 - 10.1371/journal.pone.0162459
DO - 10.1371/journal.pone.0162459
M3 - A1: Web of Science-article
SN - 1932-6203
VL - 11
JO - PLoS ONE
JF - PLoS ONE
IS - 9
M1 - 0162459
ER -