Multicenter external quality assessment program for PCR detection of Mycobacterium ulcerans in clinical and environmental specimens

Miriam Eddyani; Caroline Lavender; Willem Bram De Rijk; Pieter Bomans; Janet Fyfe; Bouke de Jong; Françoise Portaels

doi:10.1371/journal.pone.0089407

Multicenter external quality assessment program for PCR detection of Mycobacterium ulcerans in clinical and environmental specimens

Miriam Eddyani, Caroline Lavender, Willem Bram De Rijk, Pieter Bomans, Janet Fyfe, Bouke de Jong, Françoise Portaels

Mycobacteriology

Research output: Contribution to journal › A1: Web of Science-article › peer-review

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Abstract

BACKGROUND: Mycobacterium ulcerans is the causative agent of Buruli ulcer (BU), a necrotizing disease of the skin, soft tissue and bone. PCR is increasingly used in the diagnosis of BU and in research on the mode of transmission and environmental reservoir of M. ulcerans.

METHODOLOGY/PRINCIPAL FINDINGS: The aim of this study was to evaluate the performance of laboratories in detecting M. ulcerans using molecular tests in clinical and environmental samples by implementing sequential multicenter external quality assessment (EQA) programs. The second round of the clinical EQA program revealed somewhat improved performance.

CONCLUSIONS/SIGNIFICANCE: Ongoing EQA programs remain essential and continued participation in future EQA programs by laboratories involved in the molecular testing of clinical and environmental samples for M. ulcerans for diagnostic and research purposes is strongly encouraged. Broad participation in such EQA programs also benefits the harmonization of quality in the BU research community and enhances the credibility of advances made in solving the transmission enigma of M. ulcerans.

Original language	English
Journal	PLoS ONE
Volume	9
Issue number	2
Pages (from-to)	e89407
ISSN	1932-6203
DOIs	https://doi.org/10.1371/journal.pone.0089407
Publication status	Published - 2014

Keywords

Buruli Ulcer
Environment
Humans
Molecular Diagnostic Techniques
Mycobacterium ulcerans
Polymerase Chain Reaction
Quality Assurance, Health Care

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10.1371/journal.pone.0089407

2014ponee89407Final published version, 424 KB

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title = "Multicenter external quality assessment program for PCR detection of Mycobacterium ulcerans in clinical and environmental specimens",

abstract = "BACKGROUND: Mycobacterium ulcerans is the causative agent of Buruli ulcer (BU), a necrotizing disease of the skin, soft tissue and bone. PCR is increasingly used in the diagnosis of BU and in research on the mode of transmission and environmental reservoir of M. ulcerans.METHODOLOGY/PRINCIPAL FINDINGS: The aim of this study was to evaluate the performance of laboratories in detecting M. ulcerans using molecular tests in clinical and environmental samples by implementing sequential multicenter external quality assessment (EQA) programs. The second round of the clinical EQA program revealed somewhat improved performance.CONCLUSIONS/SIGNIFICANCE: Ongoing EQA programs remain essential and continued participation in future EQA programs by laboratories involved in the molecular testing of clinical and environmental samples for M. ulcerans for diagnostic and research purposes is strongly encouraged. Broad participation in such EQA programs also benefits the harmonization of quality in the BU research community and enhances the credibility of advances made in solving the transmission enigma of M. ulcerans.",

keywords = "Buruli Ulcer, Environment, Humans, Molecular Diagnostic Techniques, Mycobacterium ulcerans, Polymerase Chain Reaction, Quality Assurance, Health Care",

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T1 - Multicenter external quality assessment program for PCR detection of Mycobacterium ulcerans in clinical and environmental specimens

AU - Eddyani, Miriam

AU - Lavender, Caroline

AU - De Rijk, Willem Bram

AU - Bomans, Pieter

AU - Fyfe, Janet

AU - de Jong, Bouke

AU - Portaels, Françoise

N1 - FTX

PY - 2014

Y1 - 2014

N2 - BACKGROUND: Mycobacterium ulcerans is the causative agent of Buruli ulcer (BU), a necrotizing disease of the skin, soft tissue and bone. PCR is increasingly used in the diagnosis of BU and in research on the mode of transmission and environmental reservoir of M. ulcerans.METHODOLOGY/PRINCIPAL FINDINGS: The aim of this study was to evaluate the performance of laboratories in detecting M. ulcerans using molecular tests in clinical and environmental samples by implementing sequential multicenter external quality assessment (EQA) programs. The second round of the clinical EQA program revealed somewhat improved performance.CONCLUSIONS/SIGNIFICANCE: Ongoing EQA programs remain essential and continued participation in future EQA programs by laboratories involved in the molecular testing of clinical and environmental samples for M. ulcerans for diagnostic and research purposes is strongly encouraged. Broad participation in such EQA programs also benefits the harmonization of quality in the BU research community and enhances the credibility of advances made in solving the transmission enigma of M. ulcerans.

AB - BACKGROUND: Mycobacterium ulcerans is the causative agent of Buruli ulcer (BU), a necrotizing disease of the skin, soft tissue and bone. PCR is increasingly used in the diagnosis of BU and in research on the mode of transmission and environmental reservoir of M. ulcerans.METHODOLOGY/PRINCIPAL FINDINGS: The aim of this study was to evaluate the performance of laboratories in detecting M. ulcerans using molecular tests in clinical and environmental samples by implementing sequential multicenter external quality assessment (EQA) programs. The second round of the clinical EQA program revealed somewhat improved performance.CONCLUSIONS/SIGNIFICANCE: Ongoing EQA programs remain essential and continued participation in future EQA programs by laboratories involved in the molecular testing of clinical and environmental samples for M. ulcerans for diagnostic and research purposes is strongly encouraged. Broad participation in such EQA programs also benefits the harmonization of quality in the BU research community and enhances the credibility of advances made in solving the transmission enigma of M. ulcerans.

KW - Buruli Ulcer

KW - Environment

KW - Humans

KW - Molecular Diagnostic Techniques

KW - Mycobacterium ulcerans

KW - Polymerase Chain Reaction

KW - Quality Assurance, Health Care

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DO - 10.1371/journal.pone.0089407

M3 - A1: Web of Science-article

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SN - 1932-6203

VL - 9

SP - e89407

JO - PLoS ONE

JF - PLoS ONE

IS - 2

ER -

Multicenter external quality assessment program for PCR detection of Mycobacterium ulcerans in clinical and environmental specimens

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Keywords

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