Multilocus genotyping reveals a polyphyletic pattern among naturally antimony-resistant Leishmania braziliensis isolates from Peru

V Adaui; I Maes; T Huyse; F Van den Broeck; M Talledo; K Kuhls; S De Doncker; L Maes; A Llanos-Cuentas; G Schönian; J Arevalo; JC Dujardin

doi:10.1016/j.meegid.2011.08.008

Multilocus genotyping reveals a polyphyletic pattern among naturally antimony-resistant Leishmania braziliensis isolates from Peru

V Adaui, I Maes, T Huyse, F Van den Broeck, M Talledo, K Kuhls, S De Doncker, L Maes, A Llanos-Cuentas, G Schönian, J Arevalo, JC Dujardin

Research output: Contribution to journal › A1: Web of Science-article › peer-review

Abstract

In order to understand the epidemiological dynamics of antimonial (Sb(V)) resistance in zoonotic tegumentary leishmaniasis and its link with treatment outcome, we analyzed the population structure of 24 Peruvian Leishmania braziliensis clinical isolates with known in vitro antimony susceptibility and clinical phenotype by multilocus microsatellite typing (14 microsatellite loci). The genetic variability in the Peruvian isolates was high and the multilocus genotypes were strongly differentiated from each other. No correlation was found between the genotypes and in vitro drug susceptibility or clinical treatment outcome. The finding of a polyphyletic pattern among the Sb(V)-resistant L. braziliensis might be explained by (i) independent events of drug resistance emergence, (ii) sexual recombination and/or (iii) other phenomena mimicking recombination signals. Interestingly, the polyphyletic pattern observed here is very similar to the one we observed in the anthroponotic Leishmania donovani (Laurent et al., 2007), hereby questioning the role of transmission and/or chemotherapeutic drug pressure in the observed population structure.

Original language	English
Journal	Infection, Genetics and Evolution
Volume	11
Issue number	8
Pages (from-to)	1873-1880
Number of pages	8
ISSN	1567-1348
DOIs	https://doi.org/10.1016/j.meegid.2011.08.008
Publication status	Published - 2011

Keywords

B780-tropical-medicine
Protozoal diseases
Leishmaniasis
Tegumentary
Cutaneous
Mucosal
Zoonoses
Leishmania braziliensis
Vectors
Sandflies
Molecular epidemiology
Drug resistance
In vitro
Isolation
Multilocus
Genotyping
Antimonials
Population structure
Microsatellites
Markers
Genetic variability
Peru
America-Latin

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10.1016/j.meegid.2011.08.008

http://lib.itg.be/pdf/itg/2011/2011igev1873.pdf

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Adaui, V., Maes, I., Huyse, T., Van den Broeck, F., Talledo, M., Kuhls, K., De Doncker, S., Maes, L., Llanos-Cuentas, A., Schönian, G., Arevalo, J., & Dujardin, JC. (2011). Multilocus genotyping reveals a polyphyletic pattern among naturally antimony-resistant Leishmania braziliensis isolates from Peru. Infection, Genetics and Evolution, 11(8), 1873-1880. https://doi.org/10.1016/j.meegid.2011.08.008

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title = "Multilocus genotyping reveals a polyphyletic pattern among naturally antimony-resistant Leishmania braziliensis isolates from Peru",

abstract = "In order to understand the epidemiological dynamics of antimonial (Sb(V)) resistance in zoonotic tegumentary leishmaniasis and its link with treatment outcome, we analyzed the population structure of 24 Peruvian Leishmania braziliensis clinical isolates with known in vitro antimony susceptibility and clinical phenotype by multilocus microsatellite typing (14 microsatellite loci). The genetic variability in the Peruvian isolates was high and the multilocus genotypes were strongly differentiated from each other. No correlation was found between the genotypes and in vitro drug susceptibility or clinical treatment outcome. The finding of a polyphyletic pattern among the Sb(V)-resistant L. braziliensis might be explained by (i) independent events of drug resistance emergence, (ii) sexual recombination and/or (iii) other phenomena mimicking recombination signals. Interestingly, the polyphyletic pattern observed here is very similar to the one we observed in the anthroponotic Leishmania donovani (Laurent et al., 2007), hereby questioning the role of transmission and/or chemotherapeutic drug pressure in the observed population structure.",

keywords = "B780-tropical-medicine, Protozoal diseases, Leishmaniasis, Tegumentary, Cutaneous, Mucosal, Zoonoses, Leishmania braziliensis, Vectors, Sandflies, Molecular epidemiology, Drug resistance, In vitro, Isolation, Multilocus, Genotyping, Antimonials, Population structure, Microsatellites, Markers, Genetic variability, Peru, America-Latin",

author = "V Adaui and I Maes and T Huyse and {Van den Broeck}, F and M Talledo and K Kuhls and {De Doncker}, S and L Maes and A Llanos-Cuentas and G Sch{\"o}nian and J Arevalo and JC Dujardin",

note = "NPP; ITG-P1B; ITG-P2B; ITG-P3B; ITG-P4B; ITG-P7B; ITG-PLA; PARAS; U-PROTO; JIF; DOI; PDF; Abstract; DSPACE",

year = "2011",

doi = "10.1016/j.meegid.2011.08.008",

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Adaui, V, Maes, I, Huyse, T, Van den Broeck, F, Talledo, M, Kuhls, K, De Doncker, S, Maes, L, Llanos-Cuentas, A, Schönian, G, Arevalo, J & Dujardin, JC 2011, 'Multilocus genotyping reveals a polyphyletic pattern among naturally antimony-resistant Leishmania braziliensis isolates from Peru', Infection, Genetics and Evolution, vol. 11, no. 8, pp. 1873-1880. https://doi.org/10.1016/j.meegid.2011.08.008

TY - JOUR

T1 - Multilocus genotyping reveals a polyphyletic pattern among naturally antimony-resistant Leishmania braziliensis isolates from Peru

AU - Adaui, V

AU - Maes, I

AU - Huyse, T

AU - Van den Broeck, F

AU - Talledo, M

AU - Kuhls, K

AU - De Doncker, S

AU - Maes, L

AU - Llanos-Cuentas, A

AU - Schönian, G

AU - Arevalo, J

AU - Dujardin, JC

N1 - NPP; ITG-P1B; ITG-P2B; ITG-P3B; ITG-P4B; ITG-P7B; ITG-PLA; PARAS; U-PROTO; JIF; DOI; PDF; Abstract; DSPACE

PY - 2011

Y1 - 2011

N2 - In order to understand the epidemiological dynamics of antimonial (Sb(V)) resistance in zoonotic tegumentary leishmaniasis and its link with treatment outcome, we analyzed the population structure of 24 Peruvian Leishmania braziliensis clinical isolates with known in vitro antimony susceptibility and clinical phenotype by multilocus microsatellite typing (14 microsatellite loci). The genetic variability in the Peruvian isolates was high and the multilocus genotypes were strongly differentiated from each other. No correlation was found between the genotypes and in vitro drug susceptibility or clinical treatment outcome. The finding of a polyphyletic pattern among the Sb(V)-resistant L. braziliensis might be explained by (i) independent events of drug resistance emergence, (ii) sexual recombination and/or (iii) other phenomena mimicking recombination signals. Interestingly, the polyphyletic pattern observed here is very similar to the one we observed in the anthroponotic Leishmania donovani (Laurent et al., 2007), hereby questioning the role of transmission and/or chemotherapeutic drug pressure in the observed population structure.

AB - In order to understand the epidemiological dynamics of antimonial (Sb(V)) resistance in zoonotic tegumentary leishmaniasis and its link with treatment outcome, we analyzed the population structure of 24 Peruvian Leishmania braziliensis clinical isolates with known in vitro antimony susceptibility and clinical phenotype by multilocus microsatellite typing (14 microsatellite loci). The genetic variability in the Peruvian isolates was high and the multilocus genotypes were strongly differentiated from each other. No correlation was found between the genotypes and in vitro drug susceptibility or clinical treatment outcome. The finding of a polyphyletic pattern among the Sb(V)-resistant L. braziliensis might be explained by (i) independent events of drug resistance emergence, (ii) sexual recombination and/or (iii) other phenomena mimicking recombination signals. Interestingly, the polyphyletic pattern observed here is very similar to the one we observed in the anthroponotic Leishmania donovani (Laurent et al., 2007), hereby questioning the role of transmission and/or chemotherapeutic drug pressure in the observed population structure.

KW - B780-tropical-medicine

KW - Protozoal diseases

KW - Leishmaniasis

KW - Tegumentary

KW - Cutaneous

KW - Mucosal

KW - Zoonoses

KW - Leishmania braziliensis

KW - Vectors

KW - Sandflies

KW - Molecular epidemiology

KW - Drug resistance

KW - In vitro

KW - Isolation

KW - Multilocus

KW - Genotyping

KW - Antimonials

KW - Population structure

KW - Microsatellites

KW - Markers

KW - Genetic variability

KW - Peru

KW - America-Latin

U2 - 10.1016/j.meegid.2011.08.008

DO - 10.1016/j.meegid.2011.08.008

M3 - A1: Web of Science-article

C2 - 21871584

SN - 1567-1348

VL - 11

SP - 1873

EP - 1880

JO - Infection, Genetics and Evolution

JF - Infection, Genetics and Evolution

IS - 8

ER -

Multilocus genotyping reveals a polyphyletic pattern among naturally antimony-resistant Leishmania braziliensis isolates from Peru

Abstract

Keywords

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