Norovirus genetic diversity in children under five years old with acute diarrhea in Mozambique (2014-2015)

Jorfelia J. Chilaule, Benilde Munlela, Janet Mans, Victor V. Mabasa, Selma Marques, Adilson Fernando Loforte Bauhofer, Graziela Jane, Elda Anapakala, Fernanda Oliveira, Idalecia Cossa-Moiane, Esperanca Guimaraes, Julia Sambo, Diocreciano Matias Bero, Assucenio Chissaque, Nilsa de Deus, Maureen B. Taylor

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    Norovirus (NoV) is the second most important cause of viral diarrheal disease in children worldwide after rotavirus and is estimated to be responsible for 17% of acute diarrhea in low-income countries. This study aimed to identify and report NoV genotypes in Mozambican children under the age of five years with acute diarrhea. Between May 2014 and December 2015, stool specimens were collected within the Mozambique Diarrhea National Surveillance (ViNaDia) and tested for NoV genogroups I (GI) and II (GII) using conventional reverse transcriptase-polymerase chain reaction (RT-PCR). Partial capsid and RNA-dependent RNA polymerase (RdRp) nucleotide sequences were aligned using the Muscle tool, and phylogenetic analyses were performed using MEGA X. A total of 204 stool specimens were tested for NoV. The detection rate of NoV was 14.2% (29/204). The presence of NoV was confirmed, by real-time RT-PCR (RT-qPCR), in 24/29 (82.8%) specimens, and NoV GII predominated (70.8%; 17/24). NoV GII.4 Sydney 2012[P31] was the predominant genotype/P-type combination detected (30.4%; 7/23). This is the first study which highlights the high genetic diversity of NoV in Mozambican children and the need to establish a continuous NoV surveillance system.

    Original languageEnglish
    Article number2001
    Issue number9
    Number of pages12
    Publication statusPublished - 2022


    • Caliciviridae Infections/epidemiology
    • Child
    • Child, Preschool
    • Diarrhea/epidemiology
    • Feces
    • Gastroenteritis
    • Genetic Variation
    • Genotype
    • Humans
    • Infant
    • Mozambique/epidemiology
    • Norovirus/genetics
    • Phylogeny
    • RNA-Dependent RNA Polymerase
    • RNA-Directed DNA Polymerase/genetics


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