Norovirus genetic diversity in children under five years old with acute diarrhea in Mozambique (2014-2015)

Jorfelia J. Chilaule; Benilde Munlela; Janet Mans; Victor V. Mabasa; Selma Marques; Adilson Fernando Loforte Bauhofer; Graziela Jane; Elda Anapakala; Fernanda Oliveira; Idalecia Cossa-Moiane; Esperanca Guimaraes; Julia Sambo; Diocreciano Matias Bero; Assucenio Chissaque; Nilsa de Deus; Maureen B. Taylor

doi:10.3390/v14092001

Norovirus genetic diversity in children under five years old with acute diarrhea in Mozambique (2014-2015)

Jorfelia J. Chilaule, Benilde Munlela, Janet Mans, Victor V. Mabasa, Selma Marques, Adilson Fernando Loforte Bauhofer, Graziela Jane, Elda Anapakala, Fernanda Oliveira, Idalecia Cossa-Moiane, Esperanca Guimaraes, Julia Sambo, Diocreciano Matias Bero, Assucenio Chissaque, Nilsa de Deus, Maureen B. Taylor

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Abstract

Norovirus (NoV) is the second most important cause of viral diarrheal disease in children worldwide after rotavirus and is estimated to be responsible for 17% of acute diarrhea in low-income countries. This study aimed to identify and report NoV genotypes in Mozambican children under the age of five years with acute diarrhea. Between May 2014 and December 2015, stool specimens were collected within the Mozambique Diarrhea National Surveillance (ViNaDia) and tested for NoV genogroups I (GI) and II (GII) using conventional reverse transcriptase-polymerase chain reaction (RT-PCR). Partial capsid and RNA-dependent RNA polymerase (RdRp) nucleotide sequences were aligned using the Muscle tool, and phylogenetic analyses were performed using MEGA X. A total of 204 stool specimens were tested for NoV. The detection rate of NoV was 14.2% (29/204). The presence of NoV was confirmed, by real-time RT-PCR (RT-qPCR), in 24/29 (82.8%) specimens, and NoV GII predominated (70.8%; 17/24). NoV GII.4 Sydney 2012[P31] was the predominant genotype/P-type combination detected (30.4%; 7/23). This is the first study which highlights the high genetic diversity of NoV in Mozambican children and the need to establish a continuous NoV surveillance system.

Original language	English
Article number	2001
Journal	Viruses-Basel
Volume	14
Issue number	9
Number of pages	12
ISSN	1999-4915
DOIs	https://doi.org/10.3390/v14092001
Publication status	Published - 2022

Keywords

Caliciviridae Infections/epidemiology
Child
Child, Preschool
Diarrhea/epidemiology
Feces
Gastroenteritis
Genetic Variation
Genotype
Humans
Infant
Mozambique/epidemiology
Norovirus/genetics
Phylogeny
RNA-Dependent RNA Polymerase
RNA-Directed DNA Polymerase/genetics

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10.3390/v14092001Licence: CC BY

2022viru2001Final published version, 2.02 MBLicence: CC BY

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Chilaule, J. J., Munlela, B., Mans, J., Mabasa, V. V., Marques, S., Loforte Bauhofer, A. F., Jane, G., Anapakala, E., Oliveira, F., Cossa-Moiane, I., Guimaraes, E., Sambo, J., Bero, D. M., Chissaque, A., de Deus, N., & Taylor, M. B. (2022). Norovirus genetic diversity in children under five years old with acute diarrhea in Mozambique (2014-2015). Viruses-Basel, 14(9), Article 2001. https://doi.org/10.3390/v14092001

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title = "Norovirus genetic diversity in children under five years old with acute diarrhea in Mozambique (2014-2015)",

abstract = "Norovirus (NoV) is the second most important cause of viral diarrheal disease in children worldwide after rotavirus and is estimated to be responsible for 17% of acute diarrhea in low-income countries. This study aimed to identify and report NoV genotypes in Mozambican children under the age of five years with acute diarrhea. Between May 2014 and December 2015, stool specimens were collected within the Mozambique Diarrhea National Surveillance (ViNaDia) and tested for NoV genogroups I (GI) and II (GII) using conventional reverse transcriptase-polymerase chain reaction (RT-PCR). Partial capsid and RNA-dependent RNA polymerase (RdRp) nucleotide sequences were aligned using the Muscle tool, and phylogenetic analyses were performed using MEGA X. A total of 204 stool specimens were tested for NoV. The detection rate of NoV was 14.2% (29/204). The presence of NoV was confirmed, by real-time RT-PCR (RT-qPCR), in 24/29 (82.8%) specimens, and NoV GII predominated (70.8%; 17/24). NoV GII.4 Sydney 2012[P31] was the predominant genotype/P-type combination detected (30.4%; 7/23). This is the first study which highlights the high genetic diversity of NoV in Mozambican children and the need to establish a continuous NoV surveillance system.",

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author = "Chilaule, {Jorfelia J.} and Benilde Munlela and Janet Mans and Mabasa, {Victor V.} and Selma Marques and {Loforte Bauhofer}, {Adilson Fernando} and Graziela Jane and Elda Anapakala and Fernanda Oliveira and Idalecia Cossa-Moiane and Esperanca Guimaraes and Julia Sambo and Bero, {Diocreciano Matias} and Assucenio Chissaque and {de Deus}, Nilsa and Taylor, {Maureen B.}",

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Chilaule, JJ, Munlela, B, Mans, J, Mabasa, VV, Marques, S, Loforte Bauhofer, AF, Jane, G, Anapakala, E, Oliveira, F, Cossa-Moiane, I, Guimaraes, E, Sambo, J, Bero, DM, Chissaque, A, de Deus, N & Taylor, MB 2022, 'Norovirus genetic diversity in children under five years old with acute diarrhea in Mozambique (2014-2015)', Viruses-Basel, vol. 14, no. 9, 2001. https://doi.org/10.3390/v14092001

TY - JOUR

T1 - Norovirus genetic diversity in children under five years old with acute diarrhea in Mozambique (2014-2015)

AU - Chilaule, Jorfelia J.

AU - Munlela, Benilde

AU - Mans, Janet

AU - Mabasa, Victor V.

AU - Marques, Selma

AU - Loforte Bauhofer, Adilson Fernando

AU - Jane, Graziela

AU - Anapakala, Elda

AU - Oliveira, Fernanda

AU - Cossa-Moiane, Idalecia

AU - Guimaraes, Esperanca

AU - Sambo, Julia

AU - Bero, Diocreciano Matias

AU - Chissaque, Assucenio

AU - de Deus, Nilsa

AU - Taylor, Maureen B.

N1 - FTX; DOAJ; (CC BY 4.0)

PY - 2022

Y1 - 2022

N2 - Norovirus (NoV) is the second most important cause of viral diarrheal disease in children worldwide after rotavirus and is estimated to be responsible for 17% of acute diarrhea in low-income countries. This study aimed to identify and report NoV genotypes in Mozambican children under the age of five years with acute diarrhea. Between May 2014 and December 2015, stool specimens were collected within the Mozambique Diarrhea National Surveillance (ViNaDia) and tested for NoV genogroups I (GI) and II (GII) using conventional reverse transcriptase-polymerase chain reaction (RT-PCR). Partial capsid and RNA-dependent RNA polymerase (RdRp) nucleotide sequences were aligned using the Muscle tool, and phylogenetic analyses were performed using MEGA X. A total of 204 stool specimens were tested for NoV. The detection rate of NoV was 14.2% (29/204). The presence of NoV was confirmed, by real-time RT-PCR (RT-qPCR), in 24/29 (82.8%) specimens, and NoV GII predominated (70.8%; 17/24). NoV GII.4 Sydney 2012[P31] was the predominant genotype/P-type combination detected (30.4%; 7/23). This is the first study which highlights the high genetic diversity of NoV in Mozambican children and the need to establish a continuous NoV surveillance system.

AB - Norovirus (NoV) is the second most important cause of viral diarrheal disease in children worldwide after rotavirus and is estimated to be responsible for 17% of acute diarrhea in low-income countries. This study aimed to identify and report NoV genotypes in Mozambican children under the age of five years with acute diarrhea. Between May 2014 and December 2015, stool specimens were collected within the Mozambique Diarrhea National Surveillance (ViNaDia) and tested for NoV genogroups I (GI) and II (GII) using conventional reverse transcriptase-polymerase chain reaction (RT-PCR). Partial capsid and RNA-dependent RNA polymerase (RdRp) nucleotide sequences were aligned using the Muscle tool, and phylogenetic analyses were performed using MEGA X. A total of 204 stool specimens were tested for NoV. The detection rate of NoV was 14.2% (29/204). The presence of NoV was confirmed, by real-time RT-PCR (RT-qPCR), in 24/29 (82.8%) specimens, and NoV GII predominated (70.8%; 17/24). NoV GII.4 Sydney 2012[P31] was the predominant genotype/P-type combination detected (30.4%; 7/23). This is the first study which highlights the high genetic diversity of NoV in Mozambican children and the need to establish a continuous NoV surveillance system.

KW - Caliciviridae Infections/epidemiology

KW - Child

KW - Child, Preschool

KW - Diarrhea/epidemiology

KW - Feces

KW - Gastroenteritis

KW - Genetic Variation

KW - Genotype

KW - Humans

KW - Infant

KW - Mozambique/epidemiology

KW - Norovirus/genetics

KW - Phylogeny

KW - RNA-Dependent RNA Polymerase

KW - RNA-Directed DNA Polymerase/genetics

U2 - 10.3390/v14092001

DO - 10.3390/v14092001

M3 - A1: Web of Science-article

C2 - 36146807

SN - 1999-4915

VL - 14

JO - Viruses-Basel

JF - Viruses-Basel

IS - 9

M1 - 2001

ER -

Norovirus genetic diversity in children under five years old with acute diarrhea in Mozambique (2014-2015)

Abstract

Keywords

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