Peptidomics of neuropeptidergic tissues of the tsetse fly Glossina morsitans morsitans

Jelle Caers, Kurt Boonen, Jan Van Den Abbeele, Liesbeth Van Rompay, Liliane Schoofs, Matthias B Van Hiel

    Research output: Contribution to journalA1: Web of Science-article

    Abstract

    Neuropeptides and peptide hormones are essential signaling molecules that regulate nearly all physiological processes. The recent release of the tsetse fly genome allowed the construction of a detailed in silico neuropeptide database (International Glossina Genome Consortium, Science 344, 380-386 (2014)), as well as an in-depth mass spectrometric analysis of the most important neuropeptidergic tissues of this medically and economically important insect species. Mass spectrometric confirmation of predicted peptides is a vital step in the functional characterization of neuropeptides, as in vivo peptides can be modified, cleaved, or even mispredicted. Using a nanoscale reversed phase liquid chromatography coupled to a Q Exactive Orbitrap mass spectrometer, we detected 51 putative bioactive neuropeptides encoded by 19 precursors: adipokinetic hormone (AKH) I and II, allatostatin A and B, capability/pyrokinin (capa/PK), corazonin, calcitonin-like diuretic hormone (CT/DH), FMRFamide, hugin, leucokinin, myosuppressin, natalisin, neuropeptide-like precursor (NPLP) 1, orcokinin, pigment dispersing factor (PDF), RYamide, SIFamide, short neuropeptide F (sNPF) and tachykinin. In addition, propeptides, truncated and spacer peptides derived from seven additional precursors were found, and include the precursors of allatostatin C, crustacean cardioactive peptide, corticotropin releasing factor-like diuretic hormone (CRF/DH), ecdysis triggering hormone (ETH), ion transport peptide (ITP), neuropeptide F, and proctolin, respectively. The majority of the identified neuropeptides are present in the central nervous system, with only a limited number of peptides in the corpora cardiaca-corpora allata and midgut. Owing to the large number of identified peptides, this study can be used as a reference for comparative studies in other insects. Graphical Abstract ᅟ.

    Original languageEnglish
    JournalJournal of the American Society for Mass Spectrometry
    Volume26
    Issue number12
    Pages (from-to)2024-2038
    ISSN1044-0305
    DOIs
    Publication statusPublished - 2015

    Fingerprint

    Dive into the research topics of 'Peptidomics of neuropeptidergic tissues of the tsetse fly <i>Glossina morsitans morsitans</i>'. Together they form a unique fingerprint.

    Cite this