Phenotypic and genetic characterization of temperature-induced mutagenesis and mortality in Cupriavidus metallidurans

Rob Van Houdt, Joachim Vandecraen, Wietse Heylen, Natalie Leys, Pieter Monsieurs, Ann Provoost, Abram Aertsen

Research output: Contribution to journalA1: Web of Science-articlepeer-review

Abstract

Cupriavidus metallidurans strains display a decreased viability when incubated in rich medium at a temperature of 37°C compared to their normal growth temperature of 30°C, a phenomenon coined "temperature-induced mortality and mutagenesis" (TIMM). To scrutinize this aberrant phenotype further, the contributions of specific inducers and protective agents were determined. Different growth media, including lysogeny broth (LB) and Schatz, and components, including casamino acids, in particular amino acids (proline, cysteine, glycine, glutamine, leucine, histidine and phenylalanine) and ammonium, were found to induce TIMM at 37°C. Sorbitol was found to counteract TIMM. Furthermore, although TIMM is well conserved within the C. metallidurans species, multiple and strain-specific TIMM inducers exist. Twenty-nine percent of the TIMM survivors inherited resistance to TIMM. Whole-genome sequencing of two resistant derivatives revealed an important role of an uncharacterized oxidoreductase, indicating putative metabolic poisoning when grown in high-concentration nitrogen-containing media at 37°C.

Original languageEnglish
JournalFrontiers in Microbiology
Volume12
Pages (from-to)698330
ISSN1664-302X
DOIs
Publication statusPublished - 2021

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