Phenotypic and genotypic characterisation of Brucella strains isolated from cattle in the Gambia

AA Bankole, C Saegerman, D Berkvens, D Fretin, S Geerts, G Ieven, K Walravens

    Research output: Contribution to journalA1: Web of Science-article

    Abstract

    Thirty-five serum samples and six hygroma fluid samples were collected from sexually mature cattle in one herd with clinical signs of brucellosis (abortion and hygromas) in the Western Region of the Gambia in order to isolate and characterise Brucella species. Information on the sex, age, number of calvings, number of abortions, presence of hygromas, and presence of orchitis was also collected for each animal sampled. Twenty-six (74 per cent) of the serum samples were positive in the rose bengal test and 29 (83 per cent) were positive by indirect ELISA. Three isolates of Brucella, biotyped as Brucella abortus biovar 3, were cultured from six hygroma fluid samples. The multiple locus variable number tandem repeat analysis assay clustered the isolates as B abortus with the same profile for the three isolates, suggesting a common origin of contamination
    Original languageEnglish
    JournalVeterinary Record
    Volume166
    Issue number24
    Pages (from-to)753-756
    Number of pages4
    ISSN0042-4900
    DOIs
    Publication statusPublished - 2010

    Keywords

    • B780-tropical-medicine
    • Animal diseases
    • Brucellosis
    • Brucella abortus
    • Cattle
    • Strains
    • Isolation
    • Characterization
    • Contamination
    • Clinical manifestations
    • Hygroma
    • Abortion
    • Gambia
    • Africa-West

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