TY - JOUR
T1 - Phylogeographic analysis of dengue virus serotype 1 and cosmopolitan serotype 2 in Africa
AU - Selhorst, Philippe
AU - Lequime, Sebastian
AU - Dudas, Gytis
AU - Proesmans, Sam
AU - Lutumba, Pascal
AU - Katshongo, Freddy
AU - Ramadan, Kadrie
AU - Micalessi, Isabel
AU - Ahuka-Mundeke, Steve
AU - Vanlerberghe, Veerle
AU - Van Esbroeck, Marjan
AU - Ariën, Kevin K
N1 - FTX; DOAJ; (CC BY NC ND)
PY - 2023
Y1 - 2023
N2 - OBJECTIVES: The origin and spread of dengue virus (DENV) circulating in Africa remain poorly characterized, with African sequences representing <1% of global sequence data.METHODS: Whole genome sequencing was performed on serum samples (n = 29) from an undifferentiated fever study in 2016 in the Democratic Republic of Congo (DRC), and from febrile travelers returning from Africa. The evolutionary history of the newly acquired African DENV-1 (n = 1) and cosmopolitan genotype DENV-2 (n = 18) genomes was reconstructed using a phylogeographic, time-scaled Bayesian analysis on a curated DENV panel including all known African sequences.RESULTS: A minimum of 10 and eight introductions could be identified into Africa for DENV-1 and cosmopolitan DENV-2, respectively, almost all originating from Asia. Three introductions were previously unknown. The currently circulating virus comprises mainly the recently introduced clades and one long-established African clade. Robust geographical clustering suggests limited spread of DENV after each introduction. Our data identified the DRC as the source of the 2018 Angolan DENV-2 epidemic, and similarly, the 2013 Angolan DENV-1 outbreak as the origin of our DRC study.CONCLUSION: Active genomic surveillance of DENV in Africa at the portals of entry might help early outbreak response and limit sero- and genotype spread and human disease burden.
AB - OBJECTIVES: The origin and spread of dengue virus (DENV) circulating in Africa remain poorly characterized, with African sequences representing <1% of global sequence data.METHODS: Whole genome sequencing was performed on serum samples (n = 29) from an undifferentiated fever study in 2016 in the Democratic Republic of Congo (DRC), and from febrile travelers returning from Africa. The evolutionary history of the newly acquired African DENV-1 (n = 1) and cosmopolitan genotype DENV-2 (n = 18) genomes was reconstructed using a phylogeographic, time-scaled Bayesian analysis on a curated DENV panel including all known African sequences.RESULTS: A minimum of 10 and eight introductions could be identified into Africa for DENV-1 and cosmopolitan DENV-2, respectively, almost all originating from Asia. Three introductions were previously unknown. The currently circulating virus comprises mainly the recently introduced clades and one long-established African clade. Robust geographical clustering suggests limited spread of DENV after each introduction. Our data identified the DRC as the source of the 2018 Angolan DENV-2 epidemic, and similarly, the 2013 Angolan DENV-1 outbreak as the origin of our DRC study.CONCLUSION: Active genomic surveillance of DENV in Africa at the portals of entry might help early outbreak response and limit sero- and genotype spread and human disease burden.
KW - Humans
KW - Dengue Virus/genetics
KW - Dengue/epidemiology
KW - Serogroup
KW - Phylogeny
KW - Bayes Theorem
KW - Africa/epidemiology
KW - Genotype
KW - Disease Outbreaks
KW - Fever/epidemiology
U2 - 10.1016/j.ijid.2023.04.391
DO - 10.1016/j.ijid.2023.04.391
M3 - A1: Web of Science-article
C2 - 37088357
SN - 1201-9712
VL - 133
SP - 46
EP - 52
JO - International Journal of Infectious Diseases
JF - International Journal of Infectious Diseases
ER -