Polyelectrolyte capsules-containing HIV-1 p24 and poly I:C modulate dendritic cells to stimulate HIV-1-specific immune responses

W De Haes, S De Koker, C Pollard, D Atkinson, E Vlieghe, J Hoste, J Rejman, S De Smedt, J Grooten, G Vanham, E Van Gulck

    Research output: Contribution to journalA1: Web of Science-articlepeer-review


    Polyelectrolyte microcapsules (MCs) are potent protein delivery vehicles which can be tailored with ligands to stimulate maturation of dendritic cells (DCs). We investigated the immune stimulatory capacity of monocyte-derived DC (Mo-DC) loaded with these MCs, containing p24 antigen from human immunodeficiency virus type 1 (HIV-1) alone [p24-containing MC (MCp24)] or with the Toll-like receptor ligand 3 (TLR3) ligand poly I:C (MCp24pIC) as a maturation factor. MO-DC, loaded with MCp24pIC, upregulated CCR7, CD80, CD83, and CD86 and produced high amounts of interleukin-12 (IL-12) cytokine, to a similar extent as MCp24 in the presence of an optimized cytokine cocktail. MO-DC from HIV-infected patients under highly active antiretroviral therapy (HAART) exposed to MCp24 together with cytokine cocktail or to MCp24pIC expanded autologous p24-specific CD4(+) and CD8(+) T-cell responses as measured by interferon-gamma (IFN-gamma) and IL-2 cytokine production and secretion. In vivo relevance was shown by immunizing C57BL/6 mice with MCp24pIC, which induced both humoral and cellular p24-specific immune responses. Together these data provide a proof of principle that both antigen and DC maturation signal can be delivered as a complex with polyelectrolyte capsules to stimulate virus-specific T cells both in vitro and in vivo. Polyelectrolyte MCs could be useful for in vivo immunization in HIV-1 and other infections
    Original languageEnglish
    JournalMolecular Therapy
    Issue number7
    Pages (from-to)1408-1416
    Number of pages9
    Publication statusPublished - 2010


    • B780-tropical-medicine
    • Viral diseases
    • HIV-1
    • AIDS
    • Monocytes
    • Dendritic cells
    • Capacity
    • Immune reconstitution
    • P24 antigen
    • Cytokine
    • CD4-positive-T-lymphocytes
    • CD8-positive-T-lymphocytes
    • In vivo
    • In vitro


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