TY - JOUR
T1 - Porcine cysticercosis
T2 - Possible cross-reactivity of to GP50 antigen in the enzyme-linked immunoelectrotransfer blot assay
AU - Muro, Claudio
AU - Gomez-Puerta, Luis A
AU - Flecker, Robert H
AU - Gamboa, Ricardo
AU - Barreto, Percy Vilchez
AU - Dorny, Pierre
AU - Tsang, Victor C W
AU - Gilman, Robert H
AU - Gonzalez, Armando E
AU - Garcia, Hector H
AU - O'Neal, Seth E
AU - For The Cysticercosis Working Group In Peru, null
N1 - PPU
PY - 2017
Y1 - 2017
N2 - The lentil lectin glycoprotein enzyme-linked immunoelectrotransfer blot (LLGP EITB, reported sensitivity 99% and specificity 100%) is used as a serologic marker of exposure to Taenia solium in pigs. However, only a limited number of parasites have been evaluated for cross reactivity. Pigs may host other related cestode infections, including Taenia hydatigena, which have not been formally evaluated for cross-reactions. We investigated a corral in Tumbes, Peru, a region where a cysticercosis elimination demonstration project was completed in 2012. In this corral, 14/19 (73.7%) 6-8-week-old piglets were reactive to GP50 on LLGP EITB, and all had circulating Taenia sp. antigens. From eight necropsied piglets; four were infected with T. hydatigena metacestodes whereas none had evidence of T. solium infection. Two resident dogs were subsequently confirmed to have T. hydatigena taeniasis. These results suggest GP50 cross-reactivity in T. hydatigena-infected pigs, although controlled experimental infection is needed to confirm this hypothesis.
AB - The lentil lectin glycoprotein enzyme-linked immunoelectrotransfer blot (LLGP EITB, reported sensitivity 99% and specificity 100%) is used as a serologic marker of exposure to Taenia solium in pigs. However, only a limited number of parasites have been evaluated for cross reactivity. Pigs may host other related cestode infections, including Taenia hydatigena, which have not been formally evaluated for cross-reactions. We investigated a corral in Tumbes, Peru, a region where a cysticercosis elimination demonstration project was completed in 2012. In this corral, 14/19 (73.7%) 6-8-week-old piglets were reactive to GP50 on LLGP EITB, and all had circulating Taenia sp. antigens. From eight necropsied piglets; four were infected with T. hydatigena metacestodes whereas none had evidence of T. solium infection. Two resident dogs were subsequently confirmed to have T. hydatigena taeniasis. These results suggest GP50 cross-reactivity in T. hydatigena-infected pigs, although controlled experimental infection is needed to confirm this hypothesis.
KW - Animals
KW - Antibodies, Helminth/blood
KW - Antigens, Helminth/blood
KW - Biomarkers/blood
KW - Cross Reactions
KW - Cysticercosis/blood
KW - Dogs/parasitology
KW - Host-Parasite Interactions
KW - Nerve Tissue Proteins/blood
KW - Peru
KW - Sensitivity and Specificity
KW - Swine/parasitology
KW - Swine Diseases/blood
KW - Taenia/classification
U2 - 10.4269/ajtmh.17-0378
DO - 10.4269/ajtmh.17-0378
M3 - A1: Web of Science-article
C2 - 29016332
SN - 0002-9637
VL - 97
SP - 1830
EP - 1832
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 6
ER -