Porcine cysticercosis: Possible cross-reactivity of to GP50 antigen in the enzyme-linked immunoelectrotransfer blot assay

Claudio Muro, Luis A Gomez-Puerta, Robert H Flecker, Ricardo Gamboa, Percy Vilchez Barreto, Pierre Dorny, Victor C W Tsang, Robert H Gilman, Armando E Gonzalez, Hector H Garcia, Seth E O'Neal, For The Cysticercosis Working Group In Peru

Research output: Contribution to journalA1: Web of Science-article

Abstract

The lentil lectin glycoprotein enzyme-linked immunoelectrotransfer blot (LLGP EITB, reported sensitivity 99% and specificity 100%) is used as a serologic marker of exposure to Taenia solium in pigs. However, only a limited number of parasites have been evaluated for cross reactivity. Pigs may host other related cestode infections, including Taenia hydatigena, which have not been formally evaluated for cross-reactions. We investigated a corral in Tumbes, Peru, a region where a cysticercosis elimination demonstration project was completed in 2012. In this corral, 14/19 (73.7%) 6-8-week-old piglets were reactive to GP50 on LLGP EITB, and all had circulating Taenia sp. antigens. From eight necropsied piglets; four were infected with T. hydatigena metacestodes whereas none had evidence of T. solium infection. Two resident dogs were subsequently confirmed to have T. hydatigena taeniasis. These results suggest GP50 cross-reactivity in T. hydatigena-infected pigs, although controlled experimental infection is needed to confirm this hypothesis.

Original languageEnglish
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume97
Issue number6
Pages (from-to)1830-1832
Number of pages3
ISSN0002-9637
DOIs
Publication statusPublished - 2017

Keywords

  • Animals
  • Antibodies, Helminth/blood
  • Antigens, Helminth/blood
  • Biomarkers/blood
  • Cross Reactions
  • Cysticercosis/blood
  • Dogs/parasitology
  • Host-Parasite Interactions
  • Nerve Tissue Proteins/blood
  • Peru
  • Sensitivity and Specificity
  • Swine/parasitology
  • Swine Diseases/blood
  • Taenia/classification

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